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Set (NM_023871) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR223300
Set (Myc-DDK-tagged) - Mouse SET nuclear oncogene (Set), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Set
Synonyms 2610030F17Rik; 5730420M11Rik; AA407739; I-2PP2A; StF-IT-1; TAF-I
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223300 representing NM_023871
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCGAAGCGGCAGTCTGCGATCCTGCCTCAGCCCAAGAAACCCAGACCGGCTGCTGCCCCGAAGC
TGGAGGACAAGTCGGCCTCTCCCGGCCTGCCGAAGGGAGAAAAAGAACAGCAAGAAGCAATTGAACATAT
TGATGAAGTACAAAATGAAATAGACAGACTTAATGAACAAGCCAGTGAGGAAATTTTGAAAGTAGAACAA
AAATATAACAAACTCCGCCAACCATTTTTTCAGAAGAGGTCAGAATTGATCGCCAAAATCCCAAATTTTT
GGGTAACAACATTTGTCAACCATCCACAAGTGTCTGCACTGCTTGGGGAGGAGGACGAGGAGGCTCTGCA
TTATTTGACCAGAGTTGAAGTGACAGAATTTGAAGACATTAAATCAGGTTACAGAATAGATTTTTATTTT
GATGAAAATCCTTACTTTGAAAATAAAGTTCTCTCCAAAGAATTTCATCTGAACGAGAGTGGTGACCCGT
CTTCAAAGTCCACCGAAATCAAATGGAAATCTGGAAAGGATTTGACAAAACGCTCAAGTCAAACGCAAAA
TAAGGCCAGCAGGAAGAGGCAGCACGAAGAGCCAGAGAGCTTCTTTACCTGGTTTACTGACCATTCTGAC
GCAGGTGCTGATGAGTTAGGAGAGGTCATCAAAGATGACATCTGGCCAAATCCCTTGCAGTACTACCTGG
TTCCCGACATGGATGATGAAGAAGGAGAGGCAGAAGATGATGATGACGACGACGAAGAGGAGGAGGGGCT
GGAAGATATTGATGAAGAAGGAGATGAGGATGAAGGTGAAGAAGATGACGATGAGGATGAAGGGGAGGAA
GGAGAGGAGGACGAAGGCGAGGATGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR223300 representing NM_023871
Red=Cloning site Green=Tags(s)

MAPKRQSAILPQPKKPRPAAAPKLEDKSASPGLPKGEKEQQEAIEHIDEVQNEIDRLNEQASEEILKVEQ
KYNKLRQPFFQKRSELIAKIPNFWVTTFVNHPQVSALLGEEDEEALHYLTRVEVTEFEDIKSGYRIDFYF
DENPYFENKVLSKEFHLNESGDPSSKSTEIKWKSGKDLTKRSSQTQNKASRKRQHEEPESFFTWFTDHSD
AGADELGEVIKDDIWPNPLQYYLVPDMDDEEGEAEDDDDDDEEEEGLEDIDEEGDEDEGEEDDDEDEGEE
GEEDEGEDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023871
ORF Size 867 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023871.4, NP_076360.1
RefSeq Size 2698 bp
RefSeq ORF 870 bp
Locus ID 56086
UniProt ID Q9EQU5, Q3T9S3
Cytogenetics 2 B
MW 33.8 kDa
Summary Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_023871" in other vectors (4)
SKU Description Size Price
MC210017 Set (untagged) - Mouse SET nuclear oncogene (Set), transcript variant 1, (10ug) 10 ug
$300.00
MG223300 Set (tGFP-tagged) - Mouse SET translocation (Set), (10ug) 10 ug
$500.00
MR223300L3 Lenti ORF clone of Set (Myc-DDK-tagged) - Mouse SET nuclear oncogene (Set), transcript variant 1 10 ug
$600.00
MR223300L4 Lenti ORF clone of Set (mGFP-tagged) - Mouse SET nuclear oncogene (Set), transcript variant 1 10 ug
$600.00

Plasmid Map for MR223300

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR223300
Circular map for MR223300

file-alt Citations

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