Tfeb (NM_011549) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR223016
Tfeb (Myc-DDK-tagged) - Mouse transcription factor EB (Tcfeb), transcript variant 1
$745.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Tfeb |
| Synonyms | bHLHe35; Tcfeb |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_011549 |
| ORF Size | 1602 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_011549.2 |
| RefSeq Size | 2298 bp |
| RefSeq ORF | 1605 bp |
| Locus ID | 21425 |
| UniProt ID | Q9R210 |
| Cytogenetics | 17 23.99 cM |
| MW | 59.4 kDa |
| Summary | Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Regulates lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed:29146937).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_011549" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC217580 | Tfeb (untagged) - Mouse transcription factor EB (Tcfeb), transcript variant 1, (10ug) | 10 ug |
$747.00
|
|
| MG223016 | Tfeb (tGFP-tagged) - Mouse transcription factor EB (Tcfeb) transcript variant 1, (10ug) | 10 ug |
$945.00
|
|
| MR223016L1 | Lenti ORF clone of Tfeb (Myc-DDK-tagged) - Mouse transcription factor EB (Tcfeb), transcript variant 1 | 10 ug |
$1,045.00
|
|
| MR223016L2 | Lenti ORF clone of Tfeb (mGFP-tagged) - Mouse transcription factor EB (Tcfeb), transcript variant 1 | 10 ug |
$1,045.00
|
|
| MR223016L3 | Lenti ORF clone of Tfeb (Myc-DDK-tagged) - Mouse transcription factor EB (Tcfeb), transcript variant 1 | 10 ug |
$1,045.00
|
|
| MR223016L4 | Lenti ORF clone of Tfeb (mGFP-tagged) - Mouse transcription factor EB (Tcfeb), transcript variant 1 | 10 ug |
$1,045.00
|
|
Citations
AMPK promotes induction of the tumor suppressor FLCN through activation of TFEB independently of mTOR
Collodet, Foretz, Deak et al
FASEB J. (2019) 33 (11), 12374-12391 DOI: 10.1096/fj.201900841R
Collodet, Foretz, Deak et al
FASEB J. (2019) 33 (11), 12374-12391 DOI: 10.1096/fj.201900841R
AI Summary: OriGene’s AMPKγ1 antibody (TA300519) was used in this study to detect AMPKγ1 protein levels, contributing to the analysis of AMPK’s role in regulating FLCN expression through TFEB activation.
Snippet: …AMPKγ1 (TA300519) antibody was from OriGene Technologies (Rockville, MD, USA). A site-specific rabbit polyclonal antidody against phospho-TFEB (Ser142) was generated by YenZym Antibodies (South San Francisco, CA, USA) by immunization and affinity purification with a phosphorylated peptide of the sequence identical between human and mouse (i.e., PN-*S-PMAMLHIGSNPC-amide, where the asterisk denotes the phosphorylated residue)
Activation of TFEB ameliorates dedifferentiation of arterial smooth muscle cells and neointima formation in mice with high-fat diet
Wang, Li, Chen et al
Cell Death Dis (2019) 10 (9), 676 DOI: 10.1038/s41419-019-1931-4
Wang, Li, Chen et al
Cell Death Dis (2019) 10 (9), 676 DOI: 10.1038/s41419-019-1931-4
AI Summary: OriGene’s DDK-FLAG antibody (TA50011-100) was used in this study to detect FLAG-tagged proteins, facilitating the analysis of TFEB expression and function in smooth muscle cells.
Snippet: 6667), MMP-2 (Abcam ab92536), α-smooth muscle actin (α-SMA) (Abcam ab5694), cyclin D1 (BD 554181), CDK4 (BD 559677), TFEB (Bethyl Laboratories A303-673A), and MMP-9 (Santa Cruz sc-13520), DDK-FLAG (Origene TA50011-100). Secondary antibody for western blot: IRDye 800CW donkey anti-Mouse IgG (H + L) (LICOR 926-32212);IRDye 800CW donkey anti-rabbit IgG (H + L) (LICOR 926–32213);donk
Spatiotemporal Control of CNS Myelination by Oligodendrocyte Programmed Cell Death through the TFEB-PUMA Axis
Sun, Mulinyawe, Collins et al
Cell (2018) 175 (7), 1811-1826.e21 DOI: 10.1016/j.cell.2018.10.044
Sun, Mulinyawe, Collins et al
Cell (2018) 175 (7), 1811-1826.e21 DOI: 10.1016/j.cell.2018.10.044
AI Summary: In this study, the OriGene TFEB (NM_011549) mouse tagged ORF clone (Cat#MR223016) was used to investigate the role of TFEB in oligodendrocyte differentiation and programmed cell death, contributing to the elucidation of mechanisms regulating CNS myelination.
Snippet: pEGFP-N1-Flag vector Britton et al., 2014 Addgene Plasmid#60360 TFEB (NM_011549) mouse tagged ORF clone Origene Technologies Cat#MR223016
Buffering roles of (pro)renin receptor in starvation-induced autophagy of skeletal muscles
Mizuguchi, Yatabe, Morishima et al
Physiol Rep (2018) 6 (5) DOI: 10.14814/phy2.13587
Mizuguchi, Yatabe, Morishima et al
Physiol Rep (2018) 6 (5) DOI: 10.14814/phy2.13587
AI Summary: OriGene's mouse TFEB cDNA (catalog number MR223016) was used in this study to investigate the role of TFEB in autophagy by enabling its expression and subsequent functional analysis in cell transfection experiments.
Snippet: Plasmids and cell transfection of TFEB. Mouse TFEB cDNA (MR223016) was purchased from Origene. This cDNA was subcloned into pLVSIN-AcGFP1-C1 (Takara, Shiga, Japan) via XhoI restriction enzyme site using In-Fusion Cloning kit (Takara)
Showing 1-4 of 4 papers.
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