OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Figla (NM_012013) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR222861
Figla (Myc-DDK-tagged) - Mouse folliculogenesis specific basic helix-loop-helix (Figla)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA342301 Rabbit Polyclonal Anti-Figla Antibody
100 ul
$585.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Figla or NM_012013"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Figla
Synonyms bHLHc8; FIGalpha
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222861 representing NM_012013
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATACAGCACCTGCATCCCCAGAGCCCTTCCTGGTCACTCCGCAGGCTGAGGTGCTGGAGGAGCTGA
TTCAGGCCCAGATGGGGCCCCTGCCCCGGCTCGCCGCCATCTGTAGGCTCAAGCGCCTGCCCTCAGGCGG
CTACTCCACCACGGATGACCTGCATCTGGTGTTGGAGCGCAGGCGTGTGGCCAACGCCAAAGAGCGTGAA
CGGATAAAAAATCTCAACCGTGGCTTTGCCAAGCTGAAGGCTCTGGTGCCGTTTCTACCACAGAGCAGGA
AGCCCAGTAAAGTTGATATCCTTAAAGGTGCCACAGAATACATACAGATACTCGGCTGTGTTCTGGAAGA
AGCGAAGGTCTCAGAGAAACAAAGCCCTGAGGAGCAGACCCACAGTGGCAGACCCTCTGACCCTCATGTG
TCCTCGACTAGAGAGCTCTTGGGAAATGCTACCCAACCTACCAGCTGTGCGAGTGGCTTGAAGAAAGAAG
AGGAGGGGCCTTGGGCGTATGCTGGCCATAGTGAGCCATTGTACAGCTACCATCAGAGCACAGTACCCGA
GACTAGGAGTTACTTCACTCAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR222861 representing NM_012013
Red=Cloning site Green=Tags(s)

MDTAPASPEPFLVTPQAEVLEELIQAQMGPLPRLAAICRLKRLPSGGYSTTDDLHLVLERRRVANAKERE
RIKNLNRGFAKLKALVPFLPQSRKPSKVDILKGATEYIQILGCVLEEAKVSEKQSPEEQTHSGRPSDPHV
SSTRELLGNATQPTSCASGLKKEEEGPWAYAGHSEPLYSYHQSTVPETRSYFTH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012013
ORF Size 582 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012013.1, NP_036143.1
RefSeq Size 759 bp
RefSeq ORF 585 bp
Locus ID 26910
UniProt ID O55208
Cytogenetics 6 C3
MW 22 kDa
Summary Germ-line specific transcription factor implicated in postnatal oocyte-specific gene expression. Plays a key regulatory role in the expression of multiple oocyte-specific genes, including those that initiate folliculogenesis and those that encode the zona pellucida (ZP1, ZP2 and ZP3) required for fertilization and early embryonic survival. Essential for oocytes to survive and form primordial follicles. The persistence of FIGLA in adult females suggests that it may regulate additional pathways that are essential for normal ovarian development. Binds to the E-box (5'-CANNTG-3') of the ZPs (ZP1, ZP2, ZP3) promoters.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_012013" in other vectors (4)
SKU Description Size Price
MC209748 Figla (untagged) - Mouse folliculogenesis specific basic helix-loop-helix (Figla), (10ug) 10 ug
$330.00
MG222861 Figla (tGFP-tagged) - Mouse folliculogenesis specific basic helix-loop-helix (Figla), (10ug) 10 ug
$500.00
MR222861L3 Lenti ORF clone of Figla (Myc-DDK-tagged) - Mouse folliculogenesis specific basic helix-loop-helix (Figla) 10 ug
$600.00
MR222861L4 Lenti ORF clone of Figla (mGFP-tagged) - Mouse folliculogenesis specific basic helix-loop-helix (Figla) 10 ug
$600.00

Plasmid Map for MR222861

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR222861
Circular map for MR222861

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products