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Adgra2 (NM_054044) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR222754
Gpr124 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 124 (Gpr124)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $1,158.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Adgra2
Synonyms 8430414O08Rik; 9530074E10Rik; Gpr124; mKIAA1531; Tem5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222754 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGCGGGGGGACGCAGGATGCCGGTGCCTCCCGCGCGTTTGCTGCTGCTGCCGCTGCTGCCTTGTC
TTCTGCTCCTGGCTCCTGGAACTCGGGGTGCGCCTGGCTGCCCGGTCCCTATCCGCGGTTGCAAGTGCTC
TGGGGAGCGGCCCAAGGGACTAAGTGGCGGCGCCCACAACCCGGCTCGAAGGAGGGTGGTGTGCGGCGGT
GGGGATCTCCCCGAACCTCCAGATCCCGGCCTTCTGCCAAACGGCACCATCACCTTGCTCTTGAGCAACA
ACAAGATTACTGGGCTCCGCAATGGATCCTTCTTGGGACTGTCCCTGTTGGAGAAGTTGGACCTGAGGAG
CAATGTCATCAGCACTGTGCAGCCTGGAGCCTTCCTAGGTCTGGGAGAGCTAAAACGCTTAGATCTCTCC
AACAATCGGATTGGCTGTCTCACCTCTGAGACATTTCAAGGGCTCCCTAGACTTCTCAGACTAAACATAT
CTGGAAACATCTACTCTAGTCTGCAACCTGGGGTCTTTGATGAGCTGCCAGCCCTTAAGATTGTGGACTT
TGGTACTGAGTTTCTGACCTGTGACTGCCGCCTGCGCTGGCTGCTGCCCTGGGCCCGGAATCACTCCCTG
CAGCTGTCTGAGCGCACACTCTGTGCCTACCCCAGTGCCCTGCACGCCCATGCCCTGAGCAGCCTCCAGG
AGTCCCAGCTTCGCTGTGAAGGGGCCCTGGAACTGCACACCCACTACCTCATCCCATCCCTTCGCCAAGT
GGTGTTCCAGGGTGACCGCCTGCCCTTCCAGTGCTCAGCCAGCTACTTGGGCAACGATACCCGGATCCAC
TGGTACCACAATGGGGCTCCTATGGAGAGCGATGAGCAGGCCGGCATCGTCCTTGCTGAAAACCTTATCC
ATGATTGCACCTTCATCACCAGTGAGCTGACCCTGTCTCACATTGGTGTGTGGGCCTCTGGTGAATGGGA
GTGCTCCGTGTCCACAGTCCAAGGCAACACCAGCAAGAAGGTGGAGATAGTAGTGCTGGAGACCTCTGCC
TCCTACTGCCCTGCAGAGCGGGTGACCAACAACCGTGGGGACTTCAGGTGGCCCCGAACCTTGGCTGGCA
TCACGGCTTACCAGTCCTGTTTACAGTACCCCTTCACCTCTGTGCCCTTGAGTGGGGGAGCCCCGGGTAC
CCGAGCCTCACGCAGGTGTGACCGAGCTGGCCGCTGGGAGCCAGGGGACTACTCGCACTGTCTGTACACC
AATGACATCACTCGGGTGCTCTACACCTTTGTGCTGATGCCCATCAACGCCTCCAATGCATTGACGTTGG
CCCACCAGCTGCGAGTGTATACCGCAGAGGCCGCCAGCTTCTCAGACATGATGGACGTGGTCTATGTGGC
TCAGATGATCCAGAAGTTTTTGGGTTACGTTGACCAGATCAAGGAGCTGGTGGAGGTGATGGTGGACATG
GCCAGCAACCTGATGCTGGTGGATGAGCACCTTCTGTGGCTGGCCCAGAGAGAAGACAAAGCCTGCAGTG
GCATTGTGGGTGCCCTGGAGCGAATCGGAGGAGCTGCTCTTAGCCCCCATGCCCAGCACATCTCTGTGAA
TTCAAGAAATGTGGCACTGGAGGCCTACCTCATCAAGCCTCACAGCTACGTGGGTCTGACTTGCACGGCT
TTCCAGAGGAGGGAGGTAGGAGTGTCGGGTGCACAGCCAAGCAGCGTCGGCCAGGATGCCCCAGTGGAGC
CCGAACCCCTAGCTGATCAGCAGCTTAGGTTCCGCTGCACCACTGGGAGGCCCAACATTTCTCTGTCATC
CTTCCACATCAAGAATAGCGTGGCCCTGGCCTCCATCCAGCTGCCCCCCAGCCTGTTCTCAACCCTTCCG
GCTGCCCTGGCTCCCCCAGTCCCTCCAGATTGCACCCTGCAACTGCTGGTCTTCAGAAATGGCCGTCTTT
TCCGCAGCCACGGCAACAACACTTCCCGTCCTGGAGCAGCTGGGCCTGGCAAGAGGCGTGGTGTGGCCAC
CCCAGTCATATTTGCAGGAACCAGTGGCTGTGGTGTGGGAAACTTGACGGAGCCCGTGGCTGTGTCACTG
AGGCACTGGGCTGAAGGAGCTGACCCCATGGCAGCTTGGTGGAACCAGGACGGGCCTGGAGGCTGGAGTT
CTGAAGGCTGCAGGCTCCGCTACAGCCAGCCCAACGTCAGCTCCCTGTACTGCCAGCACTTGGGCAATGT
GGCCGTGCTTATGGAGCTGAATGCATTCCCGCGGGAGGCAGGAGGCTCTGGGGCTGGGTTGCATCCCGTC
GTGTACCCCTGCACGGCTTTGCTGCTACTCTGTCTCTTCTCCACCATCATCACGTACATCCTCAACCACA
GCTCCATCCACGTGTCCCGGAAGGGTTGGCACATGCTGCTGAACCTTTGTTTCCATATGGCCATGACCTC
TGCTGTCTTCGTGGGGGGCGTCACGCTCACCAACTACCAAATGGTTTGTCAAGCGGTGGGCATCACTCTG
CACTACTCTTCCTTGTCGTCACTGCTCTGGATGGGGGTCAAGGCTCGAGTCCTCCACAAGGAGCTTAGTT
GGAGGGCACCCCCTCTGGAAGAAGGGGAAGCAGCTCCGCCTGGTCCTCGCCCCATGCTCCGGTTCTACTT
GATTGCTGGAGGGATCCCCCTCATAATCTGCGGCATCACCGCTGCGGTCAACATCCACAACTACCGGGAC
CACAGTCCCTATTGTTGGCTGGTGTGGCGTCCAAGCCTTGGTGCCTTCTACATACCGGTGGCGTTGATTC
TGCCTATCACCTGGATCTACTTCTTGTGTGCAGGCCTTCACTTACGGAGCCATGTGGCCCAGAATCCAAA
GCAGGGTAACAGGATCTCTCTGGAGCCAGGGGAAGAGCTGAGGGGTTCCACCAGGCTCAGGAGTAGTGGC
GTCCTCCTGAATGACTCTGGTTCTCTTTTGGCTACAGTTAGCGCAGGAGTAGGGACACCTGCGCCCCCAG
AGGATGGTGATGGCGTATATTCTCCTGGAGTCCAGCTGGGGGCGCTGATGACCACGCATTTCCTGTACCT
GGCTATGTGGGCTTGTGGTGCCTTGGCGGTGTCTCAGCGCTGGCTGCCCCGAGTGGTGTGTAGCTGTCTG
TACGGCGTGGCAGCTTCAGCTCTTGGTCTGTTTGTCTTCACTCACCACTGTGCCAGACGTAGAGATGTCC
GGGCTTCCTGGCGCGCCTGCTGCCCTCCTGCTTCGCCCTCGGCCTCCCATGTCCCAGCCCGGGCCCTGCC
GACTGCTACAGAGGATGGATCCCCAGTGTTGGGGGAGGGACCAGCCTCTCTCAAGTCCTCCCCAAGTGGC
AGCAGTGGCCGCGCGCCGCCACCCCCCTGCAAACTCACCAATCTGCAGGTGGCCCAGAGTCAGGTGTGCG
AGGCAAGCGTGGCCGCCCGCGGAGATGGAGAGCCAGAGCCCACCGGCTCCCGTGGCAGCCTAGCTCCCCG
GCACCATAACAACCTGCATCATGGGCGCCGAGTACACAAGAGTCGGGCCAAGGGGCACCGAGCCGGAGAG
ACTGGTGGTAAGAGCCGGCTCAAGGCGTTGCGCGCGGGCACGTCCCCAGGAGCTCCCGAGCTGTTGTCCA
GTGAGAGCGGCAGTTTGCACAACAGCCCGTCTGACAGCTACCCGGGCAGCAGCCGCAACAGTCCAGGCGA
CGGTCTTCCACTCGAGGGTGAGCCCATGCTTACGCCGTCGGAGGGCAGTGACACAAGCGCCGCGCCAATC
GCTGAGACGGGGCGCCCCGGGCAGCGCCGCAGCGCCAGCCGTGACAACCTCAAGGGCAGCGGCAGCGCGC
TGGAGAGGGAGAGCAAGCGCCGCTCCTATCCGCTCAACACTACCAGTCTTAACGGTGCCCCCAAAGGGGG
CAAGTATGAGGATGCCAGTGTAACTGGTGCAGAGGCCATAGCGGGAGGCTCCATGAAGACTGGCCTCTGG
AAAAGCGAGACCACCGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR222754 protein sequence
Red=Cloning site Green=Tags(s)

MGAGGRRMPVPPARLLLLPLLPCLLLLAPGTRGAPGCPVPIRGCKCSGERPKGLSGGAHNPARRRVVCGG
GDLPEPPDPGLLPNGTITLLLSNNKITGLRNGSFLGLSLLEKLDLRSNVISTVQPGAFLGLGELKRLDLS
NNRIGCLTSETFQGLPRLLRLNISGNIYSSLQPGVFDELPALKIVDFGTEFLTCDCRLRWLLPWARNHSL
QLSERTLCAYPSALHAHALSSLQESQLRCEGALELHTHYLIPSLRQVVFQGDRLPFQCSASYLGNDTRIH
WYHNGAPMESDEQAGIVLAENLIHDCTFITSELTLSHIGVWASGEWECSVSTVQGNTSKKVEIVVLETSA
SYCPAERVTNNRGDFRWPRTLAGITAYQSCLQYPFTSVPLSGGAPGTRASRRCDRAGRWEPGDYSHCLYT
NDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDM
ASNLMLVDEHLLWLAQREDKACSGIVGALERIGGAALSPHAQHISVNSRNVALEAYLIKPHSYVGLTCTA
FQRREVGVSGAQPSSVGQDAPVEPEPLADQQLRFRCTTGRPNISLSSFHIKNSVALASIQLPPSLFSTLP
AALAPPVPPDCTLQLLVFRNGRLFRSHGNNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSL
RHWAEGADPMAAWWNQDGPGGWSSEGCRLRYSQPNVSSLYCQHLGNVAVLMELNAFPREAGGSGAGLHPV
VYPCTALLLLCLFSTIITYILNHSSIHVSRKGWHMLLNLCFHMAMTSAVFVGGVTLTNYQMVCQAVGITL
HYSSLSSLLWMGVKARVLHKELSWRAPPLEEGEAAPPGPRPMLRFYLIAGGIPLIICGITAAVNIHNYRD
HSPYCWLVWRPSLGAFYIPVALILPITWIYFLCAGLHLRSHVAQNPKQGNRISLEPGEELRGSTRLRSSG
VLLNDSGSLLATVSAGVGTPAPPEDGDGVYSPGVQLGALMTTHFLYLAMWACGALAVSQRWLPRVVCSCL
YGVAASALGLFVFTHHCARRRDVRASWRACCPPASPSASHVPARALPTATEDGSPVLGEGPASLKSSPSG
SSGRAPPPPCKLTNLQVAQSQVCEASVAARGDGEPEPTGSRGSLAPRHHNNLHHGRRVHKSRAKGHRAGE
TGGKSRLKALRAGTSPGAPELLSSESGSLHNSPSDSYPGSSRNSPGDGLPLEGEPMLTPSEGSDTSAAPI
AETGRPGQRRSASRDNLKGSGSALERESKRRSYPLNTTSLNGAPKGGKYEDASVTGAEAIAGGSMKTGLW
KSETTV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_054044
ORF Size 4008 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_054044.2, NP_473385.2
RefSeq Size 5520 bp
RefSeq ORF 4011 bp
Locus ID 78560
UniProt ID Q91ZV8
Cytogenetics 8 A2
MW 142.5 kDa
Summary Endothelial receptor which functions together with RECK to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed:25373781, PubMed:25558062, PubMed:28803732). Plays a key role in Wnt7-specific responses, such as endothelial cell sprouting and migration in the forebrain and neural tube, and establishment of the blood-brain barrier (PubMed:21071672, PubMed:21282641, PubMed:21421844, PubMed:25373781, PubMed:28288111). Acts as a Wnt7-specific coactivator of canonical Wnt signaling: required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (By similarity). ADGRA2-tethering function does not rely on its G-protein coupled receptor (GPCR) structure but instead on its combined capacity to interact with RECK extracellularly and recruit the Dishevelled scaffolding protein intracellularly (By similarity). Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate (By similarity).UniProtKB/Swiss-Prot Function
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"NM_054044" in other vectors (4)
SKU Description Size Price
MC224269 Gpr124 (untagged) - Mouse G protein-coupled receptor 124 (Gpr124), (10ug) 10 ug
$1,258.00
MG222754 Gpr124 (tGFP-tagged) - Mouse G protein-coupled receptor 124 (Gpr124), (10ug) 10 ug
$1,358.00
MR222754L3 Lenti ORF clone of Gpr124 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 124 (Gpr124) 10 ug
$1,458.00
MR222754L4 Lenti ORF clone of Gpr124 (mGFP-tagged) - Mouse G protein-coupled receptor 124 (Gpr124) 10 ug
$1,458.00

file-alt Citations

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