Dusp6 (NM_026268) Mouse Tagged ORF Clone
$289.00
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Dusp6 |
| Synonyms | 1300019I03Rik; MKP-3; MKP3; PYST1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_026268 |
| ORF Size | 1143 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_026268.3, NP_080544.1 |
| RefSeq Size | 2814 bp |
| RefSeq ORF | 1146 bp |
| Locus ID | 67603 |
| UniProt ID | Q9DBB1 |
| Cytogenetics | 10 D1 |
| MW | 42.9 kDa |
| Summary | Inactivates MAP kinases. Has a specificity for the ERK family (By similarity). Plays an important role in alleviating acute postoperative pain (PubMed:24155322, PubMed:28405172). Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia (PubMed:24155322). Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain (PubMed:28405172).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_026268" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG222688 | Dusp6 (tGFP-tagged) - Mouse dual specificity phosphatase 6 (Dusp6), (10ug) | 10 ug |
$657.00
|
|
| MR222688L3 | Lenti ORF clone of Dusp6 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 6 (Dusp6) | 10 ug |
$757.00
|
|
| MR222688L4 | Lenti ORF clone of Dusp6 (mGFP-tagged) - Mouse dual specificity phosphatase 6 (Dusp6) | 10 ug |
$757.00
|
|
Citations
Dynamic ROS Control by TIGAR Regulates the Initiation and Progression of Pancreatic Cancer
Cheung, DeNicola, Nixon et al
Cancer Cell (2020) 37 (2), 168-182.e4 DOI: 10.1016/j.ccell.2019.12.012
Cheung, DeNicola, Nixon et al
Cancer Cell (2020) 37 (2), 168-182.e4 DOI: 10.1016/j.ccell.2019.12.012
Cited by 248
AI Summary: In this study, OriGene’s siRNA targeting ERK1/2 (cat# SR412074, cat# 412814) and the pCMV6-Entry DUSP6 Myc-DDK tagged plasmid (cat# MR222688) were used to manipulate gene expression and investigate the molecular mechanisms by which ROS regulation via TIGAR influences pancreatic cancer initiation and progression.
Snippet: …Sur;siRNA targeting ERK1/2 and corresponding non targeting controls Origene cat# SR412074, cat# 412814 Recombinant DNA pCMV6-Entry DUSP6 Myc-DDK tagged Origene cat# MR222688 pCMV6-Entry Myc-DDK tagged empty vector This paper NA Software and Algorithms Prism 7 GraphPad https://www.graphpad.com/scientific-software/prism/;RRID: SCR_002798
Dusp6 is a genetic modifier of growth through enhanced ERK activity
Vo, Swaggart, Woo et al
Hum Mol Genet (2019) 28 (2), 279-289 DOI: 10.1093/hmg/ddy349
Vo, Swaggart, Woo et al
Hum Mol Genet (2019) 28 (2), 279-289 DOI: 10.1093/hmg/ddy349
Cited by 17
Sex-specific transcriptional signatures in human depression
Labonté, Engmann, Purushothaman et al
Nat Med (2017) 23 (9), 1102-1111 DOI: 10.1038/nm.4386
Labonté, Engmann, Purushothaman et al
Nat Med (2017) 23 (9), 1102-1111 DOI: 10.1038/nm.4386
Cited by 823
AI Summary: OriGene contributed to this study by providing Dusp6 shRNA (OriGene, TL513897) and scrambled shRNA (OriGene, TR30021) used for gene knockdown experiments to investigate the sex-specific role of Dusp6 in stress susceptibility and transcriptional remodeling in mouse prefrontal cortex.
Dusp6 is a genetic modifier of growth through enhanced ERK activity
Vo, Swaggart, Woo et al
Human Molecular Genetics (2018)
Vo, Swaggart, Woo et al
Human Molecular Genetics (2018)
Showing 1-4 of 4 papers.
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