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Dusp6 (NM_026268) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG222688
Dusp6 (tGFP-tagged) - Mouse dual specificity phosphatase 6 (Dusp6), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Other products for "Dusp6 or NM_026268"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dusp6
Synonyms 1300019I03Rik; MKP-3; MKP3; PYST1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG222688 representing NM_026268
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATAGATACGCTCAGACCCGTGCCTTTCGCGTCGGAAATGGCGATCTGCAAGACGGTGTCGTGGCTCA
ACGAGCAGCTGGAGCTGGGCAACGAACGGCTTCTGCTGATGGACTGCCGACCACAGGAGCTGTACGAGTC
GTCGCACATCGAATCTGCCATTAATGTGGCCATCCCGGGCATCATGCTGCGGCGTCTGCAGAAGGGCAAC
CTGCCCGTGCGTGCGCTCTTCACGCGCTGCGAGGACCGGGACCGCTTTACCAGGCGCTGCGGCACCGACA
CCGTGGTGCTGTACGACGAGAATAGCAGCGACTGGAATGAGAACACTGGTGGAGAGTCGGTCCTCGGGCT
GCTGCTCAAGAAACTCAAAGACGAGGGCTGCCGGGCGTTCTACCTGGAAGGTGGCTTCAGTAAGTTCCAG
GCCGAGTTCGCCCTGCACTGCGAGACCAATCTAGACGGCTCGTGCAGCAGCAGTTCCCCGCCTTTGCCAG
TGCTGGGGCTCGGGGGCCTGCGGATCAGCTCCGACTCTTCCTCGGACATTGAGTCTGACCTTGACCGAGA
CCCCAATAGTGCAACGGACTCTGATGGCAGCCCGCTGTCCAACAGCCAGCCTTCCTTCCCGGTGGAGATT
TTGCCCTTCCTTTACCTGGGCTGTGCCAAGGACTCGACCAACTTGGACGTGTTGGAAGAGTTTGGCATCA
AGTACATCTTGAATGTCACCCCCAATTTGCCCAATCTGTTTGAGAATGCGGGCGAGTTCAAATACAAGCA
AATTCCTATCTCGGATCACTGGAGCCAAAACCTGTCCCAGTTTTTCCCTGAGGCCATTTCTTTCATAGAT
GAAGCCCGAGGCAAAAACTGTGGTGTCCTGGTGCATTGCTTGGCAGGTATCAGCCGCTCTGTCACCGTGA
CAGTGGCGTACCTCATGCAGAAGCTCAACCTGTCCATGAACGATGCTTACGACATTGTTAAGATGAAGAA
GTCCAACATCTCCCCCAACTTCAACTTCATGGGCCAGCTGCTTGACTTCGAAAGGACCCTGGGACTCAGC
AGCCCTTGTGACAACCGCGTCCCCACTCCGCAGCTGTACTTCACCACGCCCTCCAACCAGAACGTCTACC
AGGTGGACTCCCTGCAGTCTACG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG222688 representing NM_026268
Red=Cloning site Green=Tags(s)

MIDTLRPVPFASEMAICKTVSWLNEQLELGNERLLLMDCRPQELYESSHIESAINVAIPGIMLRRLQKGN
LPVRALFTRCEDRDRFTRRCGTDTVVLYDENSSDWNENTGGESVLGLLLKKLKDEGCRAFYLEGGFSKFQ
AEFALHCETNLDGSCSSSSPPLPVLGLGGLRISSDSSSDIESDLDRDPNSATDSDGSPLSNSQPSFPVEI
LPFLYLGCAKDSTNLDVLEEFGIKYILNVTPNLPNLFENAGEFKYKQIPISDHWSQNLSQFFPEAISFID
EARGKNCGVLVHCLAGISRSVTVTVAYLMQKLNLSMNDAYDIVKMKKSNISPNFNFMGQLLDFERTLGLS
SPCDNRVPTPQLYFTTPSNQNVYQVDSLQST

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026268
ORF Size 1143 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026268.3, NP_080544.1
RefSeq Size 2814 bp
RefSeq ORF 1146 bp
Locus ID 67603
UniProt ID Q9DBB1
Cytogenetics 10 D1
Summary Inactivates MAP kinases. Has a specificity for the ERK family (By similarity). Plays an important role in alleviating acute postoperative pain (PubMed:24155322, PubMed:28405172). Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia (PubMed:24155322). Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain (PubMed:28405172).UniProtKB/Swiss-Prot Function
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Documents
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"NM_026268" in other vectors (3)
SKU Description Size Price
MR222688 Dusp6 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 6 (Dusp6) 10 ug
$289.00 $457.00
MR222688L3 Lenti ORF clone of Dusp6 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 6 (Dusp6) 10 ug
$757.00
MR222688L4 Lenti ORF clone of Dusp6 (mGFP-tagged) - Mouse dual specificity phosphatase 6 (Dusp6) 10 ug
$757.00

Plasmid Map for MG222688

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG222688
Circular map for MG222688

file-alt Citations

Dynamic ROS Control by TIGAR Regulates the Initiation and Progression of Pancreatic Cancer
Cheung, DeNicola, Nixon et al
Cancer Cell (2020) 37 (2), 168-182.e4 DOI: 10.1016/j.ccell.2019.12.012
Cited by 248
AI Summary: In this study, OriGene’s siRNA targeting ERK1/2 (cat# SR412074, cat# 412814) and the pCMV6-Entry DUSP6 Myc-DDK tagged plasmid (cat# MR222688) were used to manipulate gene expression and investigate the molecular mechanisms by which ROS regulation via TIGAR influences pancreatic cancer initiation and progression.
Snippet: …Sur;siRNA targeting ERK1/2 and corresponding non targeting controls Origene cat# SR412074, cat# 412814 Recombinant DNA pCMV6-Entry DUSP6 Myc-DDK tagged Origene cat# MR222688 pCMV6-Entry Myc-DDK tagged empty vector This paper NA Software and Algorithms Prism 7 GraphPad https://www.graphpad.com/scientific-software/prism/;RRID: SCR_002798
Showing 1-1 of 1 papers.
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