Gpr55 (NM_001033290) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR222607
Gpr55 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 55 (Gpr55)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpr55
Synonyms CTFL; Gm218; Lpir1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222607 representing NM_001033290
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCAGCCAGAGCGTGACAACTGCTCATTCGATTCCGTGGATAAGCTTACCAGAACCTTGCAGCTGG
CAGTCCATATCCCCACCTTCCTCCTTGGCCTGGTTCTCAACCTACTGGCCATCCGAGGCTTCAGTGCCTT
CCTAAAGAAGAGGAAGCTGGACTATATCGCCACTTCTATCTACATGATCAACTTGGCTGTTTTCGATTTA
CTGCTGGTGCTCTCCCTCCCATTCAAGATGGTCCTGCCACAAGTGGAGTCCCCCTTGCCGTCCTTCTGCA
CTCTGGTGGAGTGCCTCTACTTCATCAGCATGTATGGGAGTGTCTTCACCATCTGCTTCATCAGCCTGGA
CAGGTTCCTGGCCATCCAGTACCCGATCCTGGCTAGCCATCTCCGGTCGCCCAGGAAGACCTTTGGGATC
TGCTGCATCATCTGGATGCTGGTCTGGATCGGAAGCATCCCCATCTACACCTTCCACAGGGAAGTGGAGA
GATACAAGTGCTTTCACAACATGTCGGATGTCACCTGGAGTGCCAGTGTCTTCTTCCCCCTGGAGATCTT
TGGCTTCCTCCTTCCCATGGGCATCATGGGCTTCTGTTCCTATAGGAGTATTCACATCCTGCTGCGCCGT
CCAGACAGCACTGAGGACTGGGTCCAACAGAGAGACACTAAGGGCTGGGTACAAAAGAGAGCCTGCATCT
GGACCATTGCTACCAATCTTGTCATCTTTGTGGTCTCCTTTCTCCCAGTGCACTTGGGCTTCTTCCTGCA
GTATCTGGTGAGGAACCGCTTTATCTTGGACTGCAGAATGAAGCAGGGCATCAGCTTGTTCCTGCAGTTG
TCTCTGTGTTTCTCCAACATCAACTGCTGCCTTGATGTTTTTTGCTACTACTTTGTCATCAAAGAATTTC
GCATGCGCATCAAGGCCCACCGGCCCTCCACAATCAAGCTGGTCAACCAGGATACCATGGTCTCCAGGGG
C


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR222607 representing NM_001033290
Red=Cloning site Green=Tags(s)

MSQPERDNCSFDSVDKLTRTLQLAVHIPTFLLGLVLNLLAIRGFSAFLKKRKLDYIATSIYMINLAVFDL
LLVLSLPFKMVLPQVESPLPSFCTLVECLYFISMYGSVFTICFISLDRFLAIQYPILASHLRSPRKTFGI
CCIIWMLVWIGSIPIYTFHREVERYKCFHNMSDVTWSASVFFPLEIFGFLLPMGIMGFCSYRSIHILLRR
PDSTEDWVQQRDTKGWVQKRACIWTIATNLVIFVVSFLPVHLGFFLQYLVRNRFILDCRMKQGISLFLQL
SLCFSNINCCLDVFCYYFVIKEFRMRIKAHRPSTIKLVNQDTMVSRG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033290
ORF Size 981 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033290.2, NP_001028462.2
RefSeq Size 2550 bp
RefSeq ORF 984 bp
Locus ID 227326
UniProt ID Q3UJF0
Cytogenetics 1 C5
MW 38.5 kDa
Summary Receptor for L-alpha-lysophosphatidylinositol (LPI). LPI induces Ca(2+) release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA (By similarity). Putative cannabinoid receptor (By similarity). May play a role in bone physiology by regulating osteoclast number and function (By similarity). May be involved in hyperalgesia associated with inflammatory and neuropathic pain.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001033290" in other vectors (4)
SKU Description Size Price
MC212799 Gpr55 (untagged) - Mouse G protein-coupled receptor 55 (Gpr55), (10ug) 10 ug
$300.00
MG222607 Gpr55 (tGFP-tagged) - Mouse G protein-coupled receptor 55 (Gpr55), (10ug) 10 ug
$489.00 $500.00
MR222607L3 Lenti ORF clone of Gpr55 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 55 (Gpr55) 10 ug
$600.00
MR222607L4 Lenti ORF clone of Gpr55 (mGFP-tagged) - Mouse G protein-coupled receptor 55 (Gpr55) 10 ug
$600.00

Plasmid Map for MR222607

Download sequence for

Cloning scheme for MR222607

Circular map for MR222607

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