Sost (NM_024449) Mouse Tagged ORF Clone
$289.00
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Sost |
| Synonyms | 5430411E23Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_024449 |
| ORF Size | 633 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_024449.6 |
| RefSeq Size | 2066 bp |
| RefSeq ORF | 636 bp |
| Locus ID | 74499 |
| UniProt ID | Q99P68 |
| Cytogenetics | 11 D |
| MW | 23.5 kDa |
| Summary | Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_024449" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC211534 | Sost (untagged) - Mouse sclerostin (Sost), (10ug) | 10 ug |
$300.00
|
|
| MG222588 | Sost (tGFP-tagged) - Mouse sclerostin (Sost), (10ug) | 10 ug |
$489.00
$500.00
|
|
| MR222588L3 | Lenti ORF clone of Sost (Myc-DDK-tagged) - Mouse sclerostin (Sost) | 10 ug |
$600.00
|
|
| MR222588L4 | Lenti ORF clone of Sost (mGFP-tagged) - Mouse sclerostin (Sost) | 10 ug |
$600.00
|
|
Citations
Osteocytic Expression of Nitric Oxide and CaMKII Mediate Bone Mass and Quality
Buck
Thesis (2025)
Buck
Thesis (2025)
AI Summary: In this study, OriGene’s murine myc-tagged sclerostin plasmid (MR222588) was used to investigate the role of sclerostin in osteocyte-mediated regulation of bone mass and quality.
Snippet: Target Type Reference Number Concentration (pmol/cells) Manufacturer Murine myctagged-sclerostin Plasmid MR222588 0.05µg/40,000 Origene Scramble siRNA D-001810-01-20 4pmol/40,000 Dharmacon nNOS siRNA J-047847-09-0005 4pmol/40,000 Dharmacon iNos siRNA J-042006-05-0005 4pmol/40,000 Dharmacon
Disparate bone anabolic cues activate bone formation by regulating the rapid lysosomal degradation of sclerostin protein
Gould, Williams, Joca et al
Elife (2021) 10 DOI: 10.7554/eLife.64393
Gould, Williams, Joca et al
Elife (2021) 10 DOI: 10.7554/eLife.64393
AI Summary: In this study, OriGene products, specifically the SOST Myc-DDK tagged expression plasmid (OriGene, Cat# MR209883) and SOST shRNA (OriGene, Cat# TL516527), were used to manipulate sclerostin expression levels in cell models, enabling the investigation of how bone anabolic signals regulate sclerostin protein degradation and bone formation.
Anti-USAG-1 therapy for tooth regeneration through enhanced BMP signaling
Murashima-Suginami, Kiso, Tokita et al
Sci Adv (2021) 7 (7) DOI: 10.1126/sciadv.abf1798
Murashima-Suginami, Kiso, Tokita et al
Sci Adv (2021) 7 (7) DOI: 10.1126/sciadv.abf1798
Cited by 40
AI Summary: In this study, OriGene's expression vectors for mouse DAN family proteins were used to investigate the molecular mechanisms of USAG-1 in tooth development and regeneration.
Snippet: wich enzyme-linked immunosorbent assay (ELISA). Preparation of the E1-E4 domain of LRP6 was performed as previously reported (30). Expression vectors for mouse DAN family proteins were purchased from OriGene Technologies Inc. (Rockville, MD, USA). Generation and purification of anti–USAG-1 monoclonal antibodies The anti–USAG-1 monoclonal antibodies were generated by ITM Co. Ltd. (Matsumoto, J
Identification of Sclerostin as a Putative New Myokine Involved in the Muscle-to-Bone Crosstalk
Magarò, Bertacchini, Florio et al
Biomedicines (2021) 9 (1) DOI: 10.3390/biomedicines9010071
Magarò, Bertacchini, Florio et al
Biomedicines (2021) 9 (1) DOI: 10.3390/biomedicines9010071
Cited by 50
AI Summary: In this study, the OriGene pCMV6 expression plasmid containing the SOST gene (MR222588) was used to transiently overexpress sclerostin in mouse muscle, enabling investigation of its role in muscle-to-bone signaling.
Snippet: … Three animals (n = 3) were electroporated with the SOST vector, three (n = 3)—with the empty vector, and two (n = 2) were non-electroporated (CTRs). The pCMV6 expression plasmid (MR222588, Origene, Rockville, MD, USA) containing the SOST gene tagged with Myc-DDK was amplified in Escherichia coli. … The pCMV6 expression plasmid (MR222588, Origene, Rockville, MD, USA) containing the SOST gene tagged with Myc-DDK was amplified in Escherichia coli. Eleven days after birth, mice;The pCMV6 expression plasmid (MR222588, Origene, Rockville, MD, USA) containing the SOST gene tagged with Myc-DDK was amplified in Escherichia coli
Showing 1-4 of 4 papers.
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