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Sost (NM_024449) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG222588
Sost (tGFP-tagged) - Mouse sclerostin (Sost), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $500.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sost
Synonyms 5430411E23Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG222588 representing NM_024449
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCCCTCACTAGCCCCGTGCCTCATCTGCCTACTTGTGCACGCTGCCTTCTGTGCTGTGGAGGGCC
AGGGGTGGCAAGCCTTCAGGAATGATGCCACAGAGGTCATCCCAGGGCTTGGAGAGTACCCCGAGCCTCC
TCCTGAGAACAACCAGACCATGAACCGGGCGGAGAATGGAGGCAGACCTCCCCACCATCCCTATGACGCC
AAAGATGTGTCCGAGTACAGCTGCCGCGAGCTGCACTACACCCGCTTCCTGACAGACGGCCCATGCCGCA
GCGCCAAGCCGGTCACCGAGTTGGTGTGCTCCGGCCAGTGCGGCCCCGCGCGGCTGCTGCCCAACGCCAT
CGGGCGCGTGAAGTGGTGGCGCCCGAACGGACCGGATTTCCGCTGCATCCCGGATCGCTACCGCGCGCAG
CGGGTGCAGCTGCTGTGCCCCGGGGGCGCGGCGCCGCGCTCGCGCAAGGTGCGTCTGGTGGCCTCGTGCA
AGTGCAAGCGCCTCACCCGCTTCCACAACCAGTCGGAGCTCAAGGACTTCGGGCCGGAGACCGCGCGGCC
GCAGAAGGGTCGCAAGCCGCGGCCCGGCGCCCGGGGAGCCAAAGCCAACCAGGCGGAGCTGGAGAACGCC
TAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG222588 representing NM_024449
Red=Cloning site Green=Tags(s)

MQPSLAPCLICLLVHAAFCAVEGQGWQAFRNDATEVIPGLGEYPEPPPENNQTMNRAENGGRPPHHPYDA
KDVSEYSCRELHYTRFLTDGPCRSAKPVTELVCSGQCGPARLLPNAIGRVKWWRPNGPDFRCIPDRYRAQ
RVQLLCPGGAAPRSRKVRLVASCKCKRLTRFHNQSELKDFGPETARPQKGRKPRPGARGAKANQAELENA
Y

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024449
ORF Size 633 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024449.6
RefSeq Size 2066 bp
RefSeq ORF 636 bp
Locus ID 74499
UniProt ID Q99P68
Cytogenetics 11 D
Summary Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.UniProtKB/Swiss-Prot Function
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"NM_024449" in other vectors (4)
SKU Description Size Price
MC211534 Sost (untagged) - Mouse sclerostin (Sost), (10ug) 10 ug
$300.00
MR222588 Sost (Myc-DDK-tagged) - Mouse sclerostin (Sost) 10 ug
$289.00 $300.00
MR222588L3 Lenti ORF clone of Sost (Myc-DDK-tagged) - Mouse sclerostin (Sost) 10 ug
$600.00
MR222588L4 Lenti ORF clone of Sost (mGFP-tagged) - Mouse sclerostin (Sost) 10 ug
$600.00

Plasmid Map for MG222588

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG222588
Circular map for MG222588

file-alt Citations

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