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Cxcl11 (NM_019494) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR222244
Cxcl11 (Myc-DDK-tagged) - Mouse chemokine (C-X-C motif) ligand 11 (Cxcl11), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
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Other products for "Cxcl11 or NM_019494"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cxcl11
Synonyms b-R1; betaR1; Cxc11; H174; I-tac; Ip9; Itac; Scyb9b; Scyb11
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222244 representing NM_019494
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAGGAAGGTCACAGCCATAGCCCTGGCTGCGATCATCTGGGCCACAGCTGCTCAAGGCTTCCTTA
TGTTCAAACAGGGGCGCTGTCTTTGCATCGGCCCCGGGATGAAAGCCGTCAAAATGGCAGAGATCGAGAA
AGCTTCTGTAATTTACCCGAGTAACAGCTGCGACAAAGTTGAAGTGATTGTTACTATGAAGGCTCATAAA
CGACAAAGGTGCCTGGACCCCAGATCCAAGCAAGCTCGCCTCATAATGCAGGCAATAGAAAAAAAGAATT
TTTTAAGGCGTCAAAACATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR222244 representing NM_019494
Red=Cloning site Green=Tags(s)

MNRKVTAIALAAIIWATAAQGFLMFKQGRCLCIGPGMKAVKMAEIEKASVIYPSNSCDKVEVIVTMKAHK
RQRCLDPRSKQARLIMQAIEKKNFLRRQNM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019494
ORF Size 300 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019494.1, NP_062367.1
RefSeq Size 1597 bp
RefSeq ORF 303 bp
Locus ID 56066
UniProt ID Q9JHH5
Cytogenetics 5 E2
MW 11.7 kDa
Summary Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_019494" in other vectors (4)
SKU Description Size Price
MC210014 Cxcl11 (untagged) - Mouse chemokine (C-X-C motif) ligand 11 (Cxcl11), transcript variant 1, (10ug) 10 ug
$150.00
MG222244 Cxcl11 (tGFP-tagged) - Mouse chemokine (C-X-C motif) ligand 11 (Cxcl11), (10ug) 10 ug
$350.00
MR222244L3 Lenti ORF clone of Cxcl11 (Myc-DDK-tagged) - Mouse chemokine (C-X-C motif) ligand 11 (Cxcl11), transcript variant 1 10 ug
$450.00
MR222244L4 Lenti ORF clone of Cxcl11 (mGFP-tagged) - Mouse chemokine (C-X-C motif) ligand 11 (Cxcl11), transcript variant 1 10 ug
$450.00

Plasmid Map for MR222244

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR222244
Circular map for MR222244

file-alt Citations

Endothelial cells under therapy-induced senescence secrete CXCL11, which increases aggressiveness of breast cancer cells
Hwang, Lee, Kang et al
Cancer Lett. (2020) 490, 100-110 DOI: 10.1016/j.canlet.2020.06.019
Cited by 163
AI Summary: OriGene’s Flag-tagged CXCL11 plasmid was used in this study to investigate the role of CXCL11 in promoting aggressive behaviors in breast cancer cells exposed to secretions from therapy-induced senescent endothelial cells.
Snippet: the manufacturer's instructions. The plasmid expressing the Flag-tagged CXCL11 was purchased from OriGene (Rockville, MD, USA). 2.13. Reverse transcription-quantitative polymerase chain reaction. Total RNA was extracted
Showing 1-1 of 1 papers.
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