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Ctcfl (NM_001081387) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR222222
Ctcfl (Myc-DDK-tagged) - Mouse CCCTC-binding factor (zinc finger protein)-like (Ctcfl)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $592.00
5 Days*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ctcfl
Synonyms Boris; OTTMUSG00000016680
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222222 representing NM_001081387
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCCGCTGAGGTCCCTGTCCCTTCTGGGTACTTCACCCAGATCAAAGAGCAGAAGTTGAAGCCTG
GAGACCTAGAGGAGGAGAAAGAGGAGGACGGGGTACAAAGAGTGGAAGCCCAGGAGGGAGTTGTCAAGGA
GGTGGAGGCCGAGAACAGTTGCCTGCTTCTGGAGGCCAGGGCCCCGGTGGAGAGCGACAGGCGGATCCTG
ACCCTGCAAACGGTGCACCTGGAGTCCCAGGATGTGCACCTACAGGGGCTGGGATGGCTGAGCGTGCCAC
ACTCTGAGGAGCTTTCAGGGACGGTACCAGAGGCGGAAGGCATACTGCAGTTGCCATCCGTGCTGTGGCT
CGACCCAGAGCCCCAGCTCAGCCTTCAGCATTGCGTGACGGTCAGCATCCCGGAAGAGCTGTACCCACCA
GAGGAGCTGCAGCGGATACATTTTCACCTGCTGAGAGAGAATGTGCTAATGGCCGAGGAGAACCCAGAGT
TAACACCAGACTTGGACGAAAGCACAGCCCTGAAAAAGCCCGAAGAAGATGAAAAGGACCAGCTCCCGCC
CCAGGGAGAGACAGACAAGAGAGAAGAGAGGTTGCTCCTTCTGGAAATGAAACCAAAAGAGGGAAAAGAC
GACGAAATTGTCCTGACCATTTCCCATCTAAGCCTCGAAGAACAGCAAGATCCACCAGCGGCCAATCAGA
CAAGTGTGCCGGGAGCCAAAGCCGCAAAACCAAAACGGCGGAGGCAGACCAAGGGAAAGCCTCAGAGCTT
TCAGTGTGACACCTGCCCGTTCACTTCCTCCAAGCTCTCAACTTTCAATCGTCACATCAAAATTCACAGC
AATGAGAGGCCACACCTGTGTCACCTGTGCCTGAAGGCCTTCCGGACTGTCACTCTTCTTAGGAACCATG
TGAACACCCACACAGGAACCAGGCCCCACAAGTGCAGGGACTGCGACATGGCGTTTGTCACCAGTGGAGA
ACTCGTCCGGCACAGGCGTTACAAACACACTTATGAGAAGCCCTTCAAGTGCTCCCTGTGCAAGTACGCC
AGCGTCGAGGCAAGCAAGATGAAGCGTCACATCCGCTCACACACGGGTGAGCGTCCCTTCCAGTGTTGCC
AGTGTGCTTACGCCAGCAGGGACTCCTACAAGCTGAAGCGCCACATGAGGACACACTCAGGTGAGAAGCC
GTATGAATGTCCCACCTGTCACGTCCGGTTCACCCAGAGCGGGACCATGAAAATCCATATAGCACAGAAG
CACGGAGAGAATGTGCCCAAATACGAGTGTCCCCACTGTGCCACCATCATCGCGAGGAAGAGCGACCTGC
GTGTCCATCTGCGTAACCTGCACAGCCAGAGCCCGGAGGAGATGAAGTGCCGATACTGTCCCGCTGGCTT
CCATGAGCGCTATGCCCTCATTCAGCACCAGAGGACCCACAAGAACGAGAAGAAGTTCAAGTGCAAGCAG
TGCGATTACGCGTGCAAGCAGGAGCGATGCTTGAAGGCGCACATGCGCATGCACACAGGAGAGAAGCCCT
TCTCCTGCCTGGCCTGCAACAAGCACTTCCGACAGAAGCAGCTACTGACCGTGCACCTGAGGAAGTACCA
TGACCCGAACTTCGTCCCCAATCTGCACCTGTGCCTCAAGTGTGATAAACGTTTCTCCCGCTGGAGTAAC
CTGCAGAGACACAGAAAGAAGTGTGACCCGGAGCATGAGACGTTAGCCCCCAACAAGGACAGGAGACCAG
TGACAAGGACACAGGCCTCGGAGGGAGAAGCAGGACACAAGGAAGGGGAGCCTCAGTGCCCTGGGGAGCA
GGCTCTGGGCCACCAAGGAGAAGCAGCGGGGAGCCAGAGCCCAGACCACGGCCTTACCTGCGAGATAATC
TTTAACATGATGGATAAG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR222222 representing NM_001081387
Red=Cloning site Green=Tags(s)

MAAAEVPVPSGYFTQIKEQKLKPGDLEEEKEEDGVQRVEAQEGVVKEVEAENSCLLLEARAPVESDRRIL
TLQTVHLESQDVHLQGLGWLSVPHSEELSGTVPEAEGILQLPSVLWLDPEPQLSLQHCVTVSIPEELYPP
EELQRIHFHLLRENVLMAEENPELTPDLDESTALKKPEEDEKDQLPPQGETDKREERLLLLEMKPKEGKD
DEIVLTISHLSLEEQQDPPAANQTSVPGAKAAKPKRRRQTKGKPQSFQCDTCPFTSSKLSTFNRHIKIHS
NERPHLCHLCLKAFRTVTLLRNHVNTHTGTRPHKCRDCDMAFVTSGELVRHRRYKHTYEKPFKCSLCKYA
SVEASKMKRHIRSHTGERPFQCCQCAYASRDSYKLKRHMRTHSGEKPYECPTCHVRFTQSGTMKIHIAQK
HGENVPKYECPHCATIIARKSDLRVHLRNLHSQSPEEMKCRYCPAGFHERYALIQHQRTHKNEKKFKCKQ
CDYACKQERCLKAHMRMHTGEKPFSCLACNKHFRQKQLLTVHLRKYHDPNFVPNLHLCLKCDKRFSRWSN
LQRHRKKCDPEHETLAPNKDRRPVTRTQASEGEAGHKEGEPQCPGEQALGHQGEAAGSQSPDHGLTCEII
FNMMDK

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081387
ORF Size 1908 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081387.2, NP_001074856.1
RefSeq Size 3217 bp
RefSeq ORF 1911 bp
Locus ID 664799
UniProt ID A2APF3
Cytogenetics 2 H3
MW 73.6 kDa
Summary Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.UniProtKB/Swiss-Prot Function
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"NM_001081387" in other vectors (4)
SKU Description Size Price
MC219960 Ctcfl (untagged) - Mouse CCCTC-binding factor (zinc finger protein)-like (Ctcfl), (10ug) 10 ug
$652.00
MG222222 Ctcfl (tGFP-tagged) - Mouse CCCTC-binding factor (zinc finger protein)-like (Ctcfl), (10ug) 10 ug
$792.00
MR222222L3 Lenti ORF clone of Ctcfl (Myc-DDK-tagged) - Mouse CCCTC-binding factor (zinc finger protein)-like (Ctcfl) 10 ug
$892.00
MR222222L4 Lenti ORF clone of Ctcfl (mGFP-tagged) - Mouse CCCTC-binding factor (zinc finger protein)-like (Ctcfl) 10 ug
$892.00

Plasmid Map for MR222222

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR222222
Circular map for MR222222

file-alt Citations

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