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Ctcfl (NM_001081387) Mouse Untagged Clone

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SKU
MC219960
Ctcfl (untagged) - Mouse CCCTC-binding factor (zinc finger protein)-like (Ctcfl), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $652.00
5 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ctcfl
Synonyms Boris; OTTMUSG00000016680
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC219960 representing NM_001081387
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCCGCTGAGGTCCCTGTCCCTTCTGGGTACTTCACCCAGATCAAAGAGCAGAAGTTGAAGCCTG
GAGACCTAGAGGAGGAGAAAGAGGAGGACGGGGTACAAAGAGTGGAAGCCCAGGAGGGAGTTGTCAAGGA
GGTGGAGGCCGAGAACAGTTGCCTGCTTCTGGAGGCCAGGGCCCCGGTGGAGAGCGACAGGCGGATCCTG
ACCCTGCAAACGGTGCACCTGGAGTCCCAGGATGTGCACCTACAGGGGCTGGGATGGCTGAGCGTGCCAC
ACTCTGAGGAGCTTTCAGGGACGGTACCAGAGGCGGAAGGCATACTGCAGTTGCCATCCGTGCTGTGGCT
CGACCCAGAGCCCCAGCTCAGCCTTCAGCATTGCGTGACGGTCAGCATCCCGGAAGAGCTGTACCCACCA
GAGGAGCTGCAGCGGATACATTTTCACCTGCTGAGAGAGAATGTGCTAATGGCCGAGGAGAACCCAGAGT
TAACACCAGACTTGGACGAAAGCACAGCCCTGAAAAAGCCCGAAGAAGATGAAAAGGACCAGCTCCCGCC
CCAGGGAGAGACAGACAAGAGAGAAGAGAGGTTGCTCCTTCTGGAAATGAAACCAAAAGAGGGAAAAGAC
GACGAAATTGTCCTGACCATTTCCCATCTAAGCCTCGAAGAACAGCAAGATCCACCAGCGGCCAATCAGA
CAAGTGTGCCGGGAGCCAAAGCCGCAAAACCAAAACGGCGGAGGCAGACCAAGGGAAAGCCTCAGAGCTT
TCAGTGTGACACCTGCCCGTTCACTTCCTCCAAGCTCTCAACTTTCAATCGTCACATCAAAATTCACAGC
AATGAGAGGCCACACCTGTGTCACCTGTGCCTGAAGGCCTTCCGGACTGTCACTCTTCTTAGGAACCATG
TGAACACCCACACAGGAACCAGGCCCCACAAGTGCAGGGACTGCGACATGGCGTTTGTCACCAGTGGAGA
ACTCGTCCGGCACAGGCGTTACAAACACACTTATGAGAAGCCCTTCAAGTGCTCCCTGTGCAAGTACGCC
AGCGTCGAGGCAAGCAAGATGAAGCGTCACATCCGCTCACACACGGGTGAGCGTCCCTTCCAGTGTTGCC
AGTGTGCTTACGCCAGCAGGGACTCCTACAAGCTGAAGCGCCACATGAGGACACACTCAGGTGAGAAGCC
GTATGAATGTCCCACCTGTCACGTCCGGTTCACCCAGAGCGGGACCATGAAAATCCATATAGCACAGAAG
CACGGAGAGAATGTGCCCAAATACGAGTGTCCCCACTGTGCCACCATCATCGCGAGGAAGAGCGACCTGC
GTGTCCATCTGCGTAACCTGCACAGCCAGAGCCCGGAGGAGATGAAGTGCCGATACTGTCCCGCTGGCTT
CCATGAGCGCTATGCCCTCATTCAGCACCAGAGGACCCACAAGAACGAGAAGAAGTTCAAGTGCAAGCAG
TGCGATTACGCGTGCAAGCAGGAGCGATGCTTGAAGGCGCACATGCGCATGCACACAGGAGAGAAGCCCT
TCTCCTGCCTGGCCTGCAACAAGCACTTCCGACAGAAGCAGCTACTGACCGTGCACCTGAGGAAGTACCA
TGACCCGAACTTCGTCCCCAATCTGCACCTGTGCCTCAAGTGTGATAAACGTTTCTCCCGCTGGAGTAAC
CTGCAGAGACACAGAAAGAAGTGTGACCCGGAGCATGAGACGTTAGCCCCCAACAAGGACAGGAGACCAG
TGACAAGGACACAGGCCTCGGAGGGAGAAGCAGGACACAAGGAAGGGGAGCCTCAGTGCCCTGGGGAGCA
GGCTCTGGGCCACCAAGGAGAAGCAGCGGGGAGCCAGAGCCCAGACCACGGCCTTACCTGCGAGATAATC
TTTAACATGATGGATAAGTGA

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII |
ACCN NM_001081387
Insert Size 1911 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081387.2, NP_001074856.1
RefSeq Size 3217 bp
RefSeq ORF 1911 bp
Locus ID 664799
UniProt ID A2APF3
Cytogenetics 2 H3
Summary Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) uses an alternate in-frame 3' splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_001081387" in other vectors (4)
SKU Description Size Price
MG222222 Ctcfl (tGFP-tagged) - Mouse CCCTC-binding factor (zinc finger protein)-like (Ctcfl), (10ug) 10 ug
$792.00
MR222222 Ctcfl (Myc-DDK-tagged) - Mouse CCCTC-binding factor (zinc finger protein)-like (Ctcfl) 10 ug
$289.00 $592.00
MR222222L3 Lenti ORF clone of Ctcfl (Myc-DDK-tagged) - Mouse CCCTC-binding factor (zinc finger protein)-like (Ctcfl) 10 ug
$892.00
MR222222L4 Lenti ORF clone of Ctcfl (mGFP-tagged) - Mouse CCCTC-binding factor (zinc finger protein)-like (Ctcfl) 10 ug
$892.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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