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Ccl25 (NM_009138) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR222187
Ccl25 (Myc-DDK-tagged) - Mouse chemokine (C-C motif) ligand 25 (Ccl25), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Ccl25 or NM_009138"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ccl25
Synonyms A130072A22Rik; AI852536; CKb15; Scya25; TECK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222187 representing NM_009138
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAACTGTGGCTTTTTGCCTGCCTGGTTGCCTGTTTTGTTGGGGCCTGGATGCCGGTTGTCCATGCCC
AAGGTGCCTTTGAAGACTGCTGCCTGGGTTACCAGCACAGGATCAAATGGAATGTTCTCCGGCATGCTAG
GAATTATCACCAGCAGGAAGTGAGTGGAAGCTGCAACCTACGTGCTGTGAGATTCTACTTCCGCCAGAAA
GTAGTGTGTGGGAATCCAGAGGACATGAATGTGAAGAGGGCGATGAGAATCTTGACAGCTAGGAAAAGGC
TAGTCCACTGGAAGAGCGCCTCAGACTCTCAGACTGAAAGGAAGAAGTCAAACCATATGAAGTCCAAGGT
GGAGAACCCCAACAGTACAAGCGTGAGGAGTGCCACCCTAGGTCATCCCAGGATGGTGATGATGCCCAGA
AAGACCAACAAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR222187 representing NM_009138
Red=Cloning site Green=Tags(s)

MKLWLFACLVACFVGAWMPVVHAQGAFEDCCLGYQHRIKWNVLRHARNYHQQEVSGSCNLRAVRFYFRQK
VVCGNPEDMNVKRAMRILTARKRLVHWKSASDSQTERKKSNHMKSKVENPNSTSVRSATLGHPRMVMMPR
KTNN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009138
ORF Size 432 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009138.3, NP_033164.1
RefSeq Size 1073 bp
RefSeq ORF 435 bp
Locus ID 20300
UniProt ID O35903
Cytogenetics 8 2.01 cM
MW 17.2 kDa
Summary Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.UniProtKB/Swiss-Prot Function
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"NM_009138" in other vectors (4)
SKU Description Size Price
MC209363 Ccl25 (untagged) - Mouse chemokine (C-C motif) ligand 25 (Ccl25), transcript variant 1, (10ug) 10 ug
$165.00
MG222187 Ccl25 (tGFP-tagged) - Mouse chemokine (C-C motif) ligand 25 (Ccl25) transcript variant 1, (10ug) 10 ug
$350.00
MR222187L3 Lenti ORF clone of Ccl25 (Myc-DDK-tagged) - Mouse chemokine (C-C motif) ligand 25 (Ccl25), transcript variant 1 10 ug
$450.00
MR222187L4 Lenti ORF clone of Ccl25 (mGFP-tagged) - Mouse chemokine (C-C motif) ligand 25 (Ccl25), transcript variant 1 10 ug
$450.00

Plasmid Map for MR222187

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR222187
Circular map for MR222187

file-alt Citations

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