Lrp4 (NM_172668) Mouse Tagged ORF Clone

SKU MR222183

Lrp4 (Myc-DDK-tagged) - Mouse low density lipoprotein receptor-related protein 4 (Lrp4), transcript variant 1

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Lrp4
• Synonyms: 6430526J12Rik; D230026E03; mdig; Megf7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222183 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGGCGGTGGTGGGGCGCGCTGCTGCTTGGCGCCCTGCTCTGCGCACACGGAATAGCCAGCAGTCTGG; AGTGTGCATGTGGTCGGAGCCACTTCACATGTGCGGTGAGCGCGCTTGGCGAATGTACCTGTATCCCCGC; CCAGTGGCAGTGTGATGGAGACAATGACTGTGGAGACCACAGCGATGAGGATGGCTGTACGCTGCCTACC; TGCTCCCCTCTGGACTTCCACTGTGACAACGGCAAGTGCATCCGACGCTCCTGGGTGTGTGACGGGGACA; ATGACTGTGAAGATGACTCTGATGAGCAGGACTGTCCCCCCCGGGAGTGTGAGGAGGACGAATTCCCCTG; CCAGAACGGCTACTGCATTCGGAGTCTGTGGCACTGTGATGGGGACAACGACTGTGGTGACAACAGTGAC; GAGCAATGCGACATGCGCAAGTGCTCAGACAAGGAATTCCGCTGCAGCGACGGCAGCTGCATTGCTGAGC; ATTGGTACTGCGATGGTGACACAGACTGCAAAGATGGCTCTGATGAGGAAAGCTGCCCTTCAGCAGTACC; CTCTCCTCCTTGCAACCTGGAAGAGTTCCAGTGCGCCTATGGCCGCTGCATCCTCGACATCTACCACTGT; GACGGAGATGACGACTGTGGAGACTGGTCAGACGAGTCTGACTGCTCCTCCCACCAGCCCTGCCGCTCTG; GGGAATTCATGTGTGACAGTGGCCTGTGCATCAACTCGGGCTGGCGCTGTGATGGTGATGCAGACTGTGA; TGACCAGTCTGATGAACGCAACTGCACCACCTCCATGTGCACAGCCGAACAGTTTCGCTGTCGGTCAGGC; CGTTGCGTCCGTCTGTCCTGGCGCTGTGATGGGGAGGATGACTGTGCGGACAACAGCGATGAAGAAAACT; GTGAGAACACAGGAAGCCCCCAGTGCGCCTCTGACCAGTTCCTGTGTTGGAACGGGCGCTGTATTGGGCA; GAGGAAGCTGTGCAACGGGATCAACGACTGCGGTGACAGCAGTGACGAAAGTCCACAGCAGAACTGTCGG; CCCCGGACGGGTGAGGAGAACTGCAATGTTAACAACGGTGGCTGTGCCCAGAAGTGTCAGATGGTGCGAG; GGGCAGTGCAGTGTACCTGCCACACAGGCTACCGGCTCACAGAGGATGGGAGAACGTGCCAGGATGTAAA; CGAATGTGCTGAGGAGGGGTACTGCAGCCAGGGCTGCACAAACACTGAAGGGGCTTTCCAGTGCTGGTGT; GAAGCAGGGTATGAGCTACGGCCCGATCGGCGCAGCTGCAAAGCTCTGGGGCCAGAACCTGTGCTACTGT; TTGCCAATCGTATCGACATCCGGCAGGTGCTCCCGCACCGCTCCGAGTACACGCTGCTACTGAACAACCT; GGAGAACGCCATTGCCCTCGACTTTCATCACCGGCGGGAGCTGGTCTTCTGGTCGGATGTCACCCTGGAC; CGCATCCTCCGTGCCAACCTTAATGGCAGCAATGTAGAGGAGGTGGTGTCTACTGGGCTAGAGAGCCCAG; GGGGCCTAGCGGTGGATTGGGTCCATGACAAGCTCTACTGGACCGACTCTGGGACGTCGAGGATTGAGGT; GGCTAATCTGGATGGGGCCCACCGGAAGGTGCTGCTATGGCAGAGCCTAGAGAAGCCCAGAGCCATTGCC; CTACACCCCATGGAGGGAACCATTTATTGGACAGACTGGGGCAACACCCCCCGCATTGAGGCCTCCAGCA; TGGACGGCTCTGGGCGTCGCATCATTGCTGACACTCATCTTTTCTGGCCCAACGGCCTCACCATCGACTA; CGCTGGTCGCCGCATGTACTGGGTGGATGCTAAGCACCACGTCATCGAGAGGGCCAATCTAGATGGGAGT; CACCGCAAGGCTGTCATTAGCCAGGGCCTCCCACATCCCTTTGCCATCACAGTGTTTGAAGACAGCTTGT; ATTGGACAGACTGGCACACCAAGAGCATCAATAGTGCCAACAAGTTTACGGGGAAGAACCAGGAAATCAT; TCGCAACAAGCTCCACTTTCCTATGGACATTCATACCTTGCACCCCCAGCGCCAGCCCGCAGGGAAAAAC; CGCTGTGGGGACAACAATGGAGGTTGCACTCACCTGTGTCTACCCAGTGGCCAGAACTACACCTGTGCCT; GCCCCACCGGCTTCCGCAAGATCAACAGCCACGCCTGTGCCCAGAGTCTTGACAAGTTCCTGCTTTTTGC; CCGAAGGATGGACATCCGTCGGATCAGCTTCGACACAGAGGACCTGTCCGACGATGTCATCCCACTGGCT; GACGTGCGCAGTGCTGTGGCCCTTGACTGGGACTCCCGGGATGACCACGTGTATTGGACAGATGTCAGCA; CTGATACCATCAGCAGGGCCAAGTGGGATGGAACAGGACAGGAGGTGGTAGTGGATACCAGTTTGGAGAG; CCCTGCTGGCCTGGCAATTGATTGGGTCACCAACAAGCTCTACTGGACAGATGCAGGCACGGACAGGATC; GAAGTGGCCAACACAGATGGCAGCATGAGGACAGTGCTCATCTGGGAGAACCTTGACCGTCCTCGGGACA; TAGTGGTAGAGCCCATGGGCGGGTACATGTATTGGACTGACTGGGGTGCAAGTCCCAAGATTGAACGGGC; TGGCATGGACGCCTCCAGCCGACAAGTCATCATCTCTTCGAACCTGACCTGGCCTAACGGATTGGCTATT; GACTACGGCTCTCAGCGGCTGTACTGGGCGGATGCCGGCATGAAGACTATTGAATTTGCTGGGCTGGATG; GCAGCAAGAGGAAGGTACTAATTGGAAGCCAGCTCCCCCACCCTTTTGGGCTGACTCTATATGGACAGCG; CATCTATTGGACTGACTGGCAGACCAAGAGTATACAAAGTGCTGACCGGCTGACTGGGCTAGACCGGGAG; ACTCTGCAGGAGAACCTGGAGAACCTCATGGACATTCATGTCTTCCACCGCCAGAGGCCTCCAGTGACGA; CGCTGTGTGCCGTGGAGAATGGCGGCTGCAGCCATTTGTGTCTCAGGTCCCCAAATCCGAGCGGCTTCAG; CTGCACCTGCCCCACAGGTATCAACCTGCTACGAGATGGCAAGACATGCTCACCAGGCATGAACAGTTTC; CTCATCTTCGCCAGGAGGATTGATGTTCGCATGGTCTCCTTGGATATCCCTTATTTTGCTGATGTGGTGG; TACCCATCAACATGACCATGAAGAACACCATCGCCATTGGAGTGGATCCGCTGGAAGGAAAAGTGTACTG; GTCTGACAGCACGCTGCACAGGATCAGCCGTGCCAGCCTGGATGGCTCACAGCATGAGGACATCATCACT; ACAGGTCTGCAGACCACAGACGGGCTTGCAGTGGATGCCATCGGCCGGAAAGTGTACTGGACAGACACAG; GAACGAATCGGATTGAAGTTGGCAACCTGGATGGGTCTATGCGGAAAGTGTTGGTGTGGCAGAACCTTGA; CAGTCCCCGGGCCATTGTATTATACCATGAAATGGGGTTCATGTACTGGACAGACTGGGGGGAGAACGCC; AAATTAGAGCGTTCCGGAATGGATGGCTCAGACCGTACTGTGCTCATCAACAACAACCTAGGGTGGCCCA; ATGGACTGACTGTGGACAAGACCAGTTCCCAACTGCTGTGGGCTGATGCCCATACTGAGAGAATTGAGGT; GGCAGACCTGAATGGCGCTAATCGGCATACACTGGTGTCACCAGTCCAGCACCCATATGGCCTCACTCTG; CTTGACTCCTATATCTACTGGACTGACTGGCAGACCCGCAGCATCCACCGGGCCGACAAGAGTACTGGCA; GCAATGTCATCCTTGTGAGGTCCAACCTGCCAGGCCTCATGGACATCCAAGCTGTTGACCGGGCACAGCC; ACTGGGTTTTAATAAATGTGGCTCAAGAAATGGCGGCTGTTCCCACCTCTGTTTACCTCGGCCTTCTGGT; TTCTCCTGTGCCTGTCCCACTGGCATTCAGCTGAAGGGAGACAGGAAGACCTGTGACCCCTCTCCAGAAA; CCTACCTGCTCTTCTCTAGCCGAGGCTCTATTCGCCGTATCTCACTGGACACCGATGACCACACAGACGT; GCACGTTCCTGTTCCTGGGCTCAACAATGTCATCTCCCTAGACTATGACAGTGTGCATGGAAAGGTCTAT; TACACAGACGTGTTCCTGGATGTTATCAGGCGAGCAGACCTGAATGGCAGCAACATGGAGACTGTTATTG; GACACGGGCTTAAGACCACTGACGGGCTGGCAGTGGACTGGGTTGCCAGAAACCTGTACTGGACAGATAC; CGGCCGAAATACCATCGAAGCATCTCGGCTAGATGGATCCTGTCGCAAAGTGCTAATCAACAACAGTCTA; GACGAGCCCCGGGCCATTGCTGTTTTCCCCAGAAAGGGGTACCTCTTCTGGACAGACTGGGGCCACATTG; CCAAGATTGAACGGGCAAACCTGGATGGCTCTGAGAGGAAGGTCCTCATCAACACAGACCTAGGCTGGCC; TAACGGCCTCACCTTGGACTATGATACCCGCAGGATCTACTGGGTAGATGCACATCTGGATCGGATCGAG; AGTGCTGACCTCAATGGGAAGCTGAGGCAGGTCTTGGTCAGCCATGTGTCCCACCCCTTTGCCCTCACAC; AGCAGGACAGGTGGATCTACTGGACGGACTGGCAGACCAAATCGATCCAACGTGTCGACAAGTACTCGGG; CCGCAACAAGGAGACAGTACTGGCCAACGTGGAGGGACTTATGGATATTATTGTGGTGTCCCCTCAGCGG; CAGACAGGGACCAATGCCTGCGGTGTGAATAATGGTGGCTGTACTCACCTCTGCTTTGCCCGAGCCTCTG; ACTTTGTGTGCGCGTGTCCTGATGAGCCTGACGGTCATCCCTGCTCCCTTGTACCTGGCCTCGTACCTCC; TGCTCCCAGAGCTACCAGCATGAATGAGAAGAGCCCAGTGCTACCCAACACGCTGCCCACCACCTTACAT; TCTTCTACCACCAAGACCCGCACCTCTCTGGAGGGGGCAGGAGGAAGATGCTCTGAAAGGGATGCCCAGC; TGGGCCTCTGTGCACATTCCAATGAAGCCGTCCCTGCTGCTCCAGGTGAAGGACTGCACGTCAGCTATGC; CATCGGTGGACTCCTCAGTATCCTGCTGATTTTGTTGGTGATCGCGGCTTTGATGCTATACAGACACAGA; AAATCCAAGTTCACGGATCCTGGAATGGGAAACCTGACCTACAGCAACCCCTCCTACCGAACTTCCACTC; AGGAAGTGAAACTTGAAGCAGCTCCCAAGCCAGCCGTGTATAATCAGCTGTGCTATAAGAAAGAGGGTGG; ACCTGACCACAGCTACACCAAGGAGAAGATCAAGATTGTAGAGGGAATCCGCCTCCTGGCTGGGGATGAC; GCTGAATGGGGTGACCTCAAGCAACTGCGCAGCTCCAGGGGGGGCCTTCTACGGGATCACGTATGCATGA; AGACAGACACAGTGTCCATCCAGGCCAGCTCTGGCTCCCTAGATGACACGGAGACAGAGCAGCTGCTTCA; GGAAGAGCAGTCTGAGTGTAGCAGTGTTCACACTGCAGCCACTCCGGAGAGACGGGGCTCTCTGCCAGAC; ACAGGCTGGAAACACGAACGCAAGCTCTCCTCAGAAAGCCAGGTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222183 protein sequence; Red=Cloning site Green=Tags(s); ; MRRWWGALLLGALLCAHGIASSLECACGRSHFTCAVSALGECTCIPAQWQCDGDNDCGDHSDEDGCTLPT; CSPLDFHCDNGKCIRRSWVCDGDNDCEDDSDEQDCPPRECEEDEFPCQNGYCIRSLWHCDGDNDCGDNSD; EQCDMRKCSDKEFRCSDGSCIAEHWYCDGDTDCKDGSDEESCPSAVPSPPCNLEEFQCAYGRCILDIYHC; DGDDDCGDWSDESDCSSHQPCRSGEFMCDSGLCINSGWRCDGDADCDDQSDERNCTTSMCTAEQFRCRSG; RCVRLSWRCDGEDDCADNSDEENCENTGSPQCASDQFLCWNGRCIGQRKLCNGINDCGDSSDESPQQNCR; PRTGEENCNVNNGGCAQKCQMVRGAVQCTCHTGYRLTEDGRTCQDVNECAEEGYCSQGCTNTEGAFQCWC; EAGYELRPDRRSCKALGPEPVLLFANRIDIRQVLPHRSEYTLLLNNLENAIALDFHHRRELVFWSDVTLD; RILRANLNGSNVEEVVSTGLESPGGLAVDWVHDKLYWTDSGTSRIEVANLDGAHRKVLLWQSLEKPRAIA; LHPMEGTIYWTDWGNTPRIEASSMDGSGRRIIADTHLFWPNGLTIDYAGRRMYWVDAKHHVIERANLDGS; HRKAVISQGLPHPFAITVFEDSLYWTDWHTKSINSANKFTGKNQEIIRNKLHFPMDIHTLHPQRQPAGKN; RCGDNNGGCTHLCLPSGQNYTCACPTGFRKINSHACAQSLDKFLLFARRMDIRRISFDTEDLSDDVIPLA; DVRSAVALDWDSRDDHVYWTDVSTDTISRAKWDGTGQEVVVDTSLESPAGLAIDWVTNKLYWTDAGTDRI; EVANTDGSMRTVLIWENLDRPRDIVVEPMGGYMYWTDWGASPKIERAGMDASSRQVIISSNLTWPNGLAI; DYGSQRLYWADAGMKTIEFAGLDGSKRKVLIGSQLPHPFGLTLYGQRIYWTDWQTKSIQSADRLTGLDRE; TLQENLENLMDIHVFHRQRPPVTTLCAVENGGCSHLCLRSPNPSGFSCTCPTGINLLRDGKTCSPGMNSF; LIFARRIDVRMVSLDIPYFADVVVPINMTMKNTIAIGVDPLEGKVYWSDSTLHRISRASLDGSQHEDIIT; TGLQTTDGLAVDAIGRKVYWTDTGTNRIEVGNLDGSMRKVLVWQNLDSPRAIVLYHEMGFMYWTDWGENA; KLERSGMDGSDRTVLINNNLGWPNGLTVDKTSSQLLWADAHTERIEVADLNGANRHTLVSPVQHPYGLTL; LDSYIYWTDWQTRSIHRADKSTGSNVILVRSNLPGLMDIQAVDRAQPLGFNKCGSRNGGCSHLCLPRPSG; FSCACPTGIQLKGDRKTCDPSPETYLLFSSRGSIRRISLDTDDHTDVHVPVPGLNNVISLDYDSVHGKVY; YTDVFLDVIRRADLNGSNMETVIGHGLKTTDGLAVDWVARNLYWTDTGRNTIEASRLDGSCRKVLINNSL; DEPRAIAVFPRKGYLFWTDWGHIAKIERANLDGSERKVLINTDLGWPNGLTLDYDTRRIYWVDAHLDRIE; SADLNGKLRQVLVSHVSHPFALTQQDRWIYWTDWQTKSIQRVDKYSGRNKETVLANVEGLMDIIVVSPQR; QTGTNACGVNNGGCTHLCFARASDFVCACPDEPDGHPCSLVPGLVPPAPRATSMNEKSPVLPNTLPTTLH; SSTTKTRTSLEGAGGRCSERDAQLGLCAHSNEAVPAAPGEGLHVSYAIGGLLSILLILLVIAALMLYRHR; KSKFTDPGMGNLTYSNPSYRTSTQEVKLEAAPKPAVYNQLCYKKEGGPDHSYTKEKIKIVEGIRLLAGDD; AEWGDLKQLRSSRGGLLRDHVCMKTDTVSIQASSGSLDDTETEQLLQEEQSECSSVHTAATPERRGSLPD; TGWKHERKLSSESQV; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172668
• ORF Size: 5715 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_172668.2, NM_172668.3, NP_766256.3
• RefSeq Size: 8028 bp
• RefSeq ORF: 5718 bp
• Locus ID: 228357
• UniProt ID: Q8VI56
• Cytogenetics: 2 50.63 cM
• MW: 212 kDa
• Summary: Mediates SOST-dependent inhibition of bone formation. Functions as a specific facilitator of SOST-mediated inhibition of Wnt signaling. Plays a key role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Directly binds AGRIN and recruits it to the MUSK signaling complex. Mediates the AGRIN-induced phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Alternatively, may be involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. More generally, has been proposed to function as a cell surface endocytic receptor binding and internalizing extracellular ligands for degradation by lysosomes. Plays an essential role in the process of digit differentiation (PubMed:16517118).[UniProtKB/Swiss-Prot Function]