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Cycs (NM_007808) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR222141
Cycs (Myc-DDK-tagged) - Mouse cytochrome c, somatic (Cycs), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cycs
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222141 representing NM_007808
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTGATGTTGAAAAAGGCAAGAAGATTTTTGTTCAGAAGTGTGCCCAGTGCCACACTGTGGAAAAGG
GAGGCAAGCATAAGACTGGACCAAATCTCCACGGTCTGTTCGGGCGGAAGACAGGCCAGGCTGCTGGATT
CTCTTACACAGATGCCAACAAGAACAAAGGCATCACCTGGGGAGAGGATACCCTGATGGAGTATTTGGAG
AATCCCAAAAAGTACATCCCTGGAACAAAAATGATCTTCGCTGGAATTAAGAAGAAGGGAGAAAGGGCAG
ACCTAATAGCTTATCTTAAAAAGGCTACTAATGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR222141 representing NM_007808
Red=Cloning site Green=Tags(s)

MGDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNLHGLFGRKTGQAAGFSYTDANKNKGITWGEDTLMEYLE
NPKKYIPGTKMIFAGIKKKGERADLIAYLKKATNE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007808
ORF Size 315 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007808.5
RefSeq Size 3059 bp
RefSeq ORF 318 bp
Locus ID 13063
UniProt ID P62897
Cytogenetics 6 24.32 cM
MW 12.1 kDa
Summary Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.UniProtKB/Swiss-Prot Function
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"NM_007808" in other vectors (4)
SKU Description Size Price
MC202833 Cycs (untagged) - Mouse cytochrome c, somatic (Cycs), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$150.00
MG222141 Cycs (tGFP-tagged) - Mouse cytochrome c somatic (Cycs) nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$350.00
MR222141L3 Lenti ORF clone of Cycs (Myc-DDK-tagged) - Mouse cytochrome c, somatic (Cycs), nuclear gene encoding mitochondrial protein 10 ug
$450.00
MR222141L4 Lenti ORF clone of Cycs (mGFP-tagged) - Mouse cytochrome c, somatic (Cycs), nuclear gene encoding mitochondrial protein 10 ug
$450.00

Plasmid Map for MR222141

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR222141
Circular map for MR222141

file-alt Citations

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