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Crispld2 (NM_030209) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR222108
Crispld2 (Myc-DDK-tagged) - Mouse cysteine-rich secretory protein LCCL domain containing 2 (Crispld2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Crispld2
Synonyms 1810049K24Rik; coffeecrisp; Lcrisp2; Lgl1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222108 representing NM_030209
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTGTCTTCTGAACAATATGGTCCTGATGGGGCTGGCTCTGCTGGTCTGTGGAGTACAGGCCTTTT
TCCTTCCCAACACCACGAGCCTGGAGAAGCTGCTGAGCAAATACCAGCATGCAGAGCCACACTCACGCGT
GCGCAGGGCCATCCCCATGTCAGACCGCCAAGAGATCCTCATGCTACACAACAAGCTGCGAGGCCAGGTG
TATCCCCCTGCCTCCAACATGGAACACATGACTTGGGATGAGGAGCTAGAGAGATCTGCAGCAGCGTGGG
CACATAGATGCCTGTGGGAGCACGGGCCCGCCGGCCTTCTGAGGTCCATCGGGCAGAACCTGGCTGTGCA
CTGGGGTAGGTACCGCTCTCCTGGGTTCCATGTGCAGTCATGGTACGACGAGGTGAAGGATTACACCTAC
CCATACCCCCACGAGTGCACTCCACGGTGCCGGGAGCGGTGCTCAGGCCCCATGTGCACCCACTACACAC
AGATGGTCTGGGCCACCACCAACAAGATCGGCTGTGCCGTGCACACCTGTCGGAACATGAACGTCTGGGG
AGACACTTGGGAGAATGCCGTGTATCTCGTCTGCAATTATTCTCCCAAGGGAAACTGGATTGGCGAGGCC
CCCTACAAGCATGGCCGTCCCTGCTCTGAGTGTCCATCCAGCTACGGAGGAGGCTGCCTGAACAATCTTT
GCCACCGAGAAAAGCCTCACAAGCATAAACCAGAGGTGGATATGATGAACGAGGTGGAATCGCCCCCTGC
TCCAGAGGAAACCCATGTCTGGGTTCAGCCCAGGGTGATCAAGACCAAGAAGACCCCAGTCATCAACTTC
ATGACCCAAGTGGTCCACTGTGACACCAAGATGAAGGACTCATGCAAAGGATCCACGTGTAACAGGTACC
AGTGCCCAGCAGGCTGTCTGAGCAACAAGGCGAAGGTCTTTGGCTCTCTGTTTTATGAAAGTTCTTCCAG
CATATGCCGAGCTGCTATCCACTACGGTGTCATCGATGATCGAGGTGGCCTGGTGGATGTCACCAGGAAT
GGGATGGTACCCTTCTTTGTCAAGTCTCAGAAAAATGGCATGGAGTCCCTGAGCAAATACAAGCCGTCTA
GCTCCTTCACTGTGTCAAAAGTGACAGAGACGGCCGTGGACTGCCACGCCACGGTCGCACAGCTGTGCCC
CTTCGAGAAGCCGGCCACCCACTGCCCGAGAATCCAGTGTCCTGCGCGCTGTGGAGAAGAGCCATCCTAT
TGGGCTCCTGTGTATGGAACCAACATCTATGCTGATACTTCCAGCATTTGTAAGGCCGCTGTGCACGCAG
GTGTCATCGTTGACGAGGTTGGTGGCTATGCAGATGTGATGCCCGTGGACAAAAAGAAGAGCTACGTGGG
CTCCCTCAGGAACGGGGTGCAGTCGGAGAGCCTGAACACTCCTCAGAACGGAAACGCCTTCCGGATCTTC
GCCGTCAGGCAG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR222108 representing NM_030209
Red=Cloning site Green=Tags(s)

MSCLLNNMVLMGLALLVCGVQAFFLPNTTSLEKLLSKYQHAEPHSRVRRAIPMSDRQEILMLHNKLRGQV
YPPASNMEHMTWDEELERSAAAWAHRCLWEHGPAGLLRSIGQNLAVHWGRYRSPGFHVQSWYDEVKDYTY
PYPHECTPRCRERCSGPMCTHYTQMVWATTNKIGCAVHTCRNMNVWGDTWENAVYLVCNYSPKGNWIGEA
PYKHGRPCSECPSSYGGGCLNNLCHREKPHKHKPEVDMMNEVESPPAPEETHVWVQPRVIKTKKTPVINF
MTQVVHCDTKMKDSCKGSTCNRYQCPAGCLSNKAKVFGSLFYESSSSICRAAIHYGVIDDRGGLVDVTRN
GMVPFFVKSQKNGMESLSKYKPSSSFTVSKVTETAVDCHATVAQLCPFEKPATHCPRIQCPARCGEEPSY
WAPVYGTNIYADTSSICKAAVHAGVIVDEVGGYADVMPVDKKKSYVGSLRNGVQSESLNTPQNGNAFRIF
AVRQ

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_030209
ORF Size 1482 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030209.2
RefSeq Size 4165 bp
RefSeq ORF 1485 bp
Locus ID 78892
UniProt ID Q8BZQ2
Cytogenetics 8 E1
MW 55.8 kDa
Summary Promotes matrix assembly.UniProtKB/Swiss-Prot Function
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Documents
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"NM_030209" in other vectors (6)
SKU Description Size Price
MC216868 Crispld2 (untagged) - Mouse cysteine-rich secretory protein LCCL domain containing 2 (Crispld2), (10ug) 10 ug
$503.00
MG222108 Crispld2 (tGFP-tagged) - Mouse cysteine-rich secretory protein LCCL domain containing 2 (Crispld2), (10ug) 10 ug
$657.00
MR222108L1 Lenti ORF clone of Crispld2 (Myc-DDK-tagged) - Mouse cysteine-rich secretory protein LCCL domain containing 2 (Crispld2) 10 ug
$757.00
MR222108L2 Lenti ORF clone of Crispld2 (mGFP-tagged) - Mouse cysteine-rich secretory protein LCCL domain containing 2 (Crispld2) 10 ug
$757.00
MR222108L3 Lenti ORF clone of Crispld2 (Myc-DDK-tagged) - Mouse cysteine-rich secretory protein LCCL domain containing 2 (Crispld2) 10 ug
$757.00
MR222108L4 Lenti ORF clone of Crispld2 (mGFP-tagged) - Mouse cysteine-rich secretory protein LCCL domain containing 2 (Crispld2) 10 ug
$757.00

Plasmid Map for MR222108

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR222108
Circular map for MR222108

file-alt Citations

CRISPLD2, a novel insulin-sensitizing adipokine that alters adipocyte size
Griesel, Williams, Ahsan et al
Obesity (Silver Spring) (2025)
Cited by 1
Snippet: cDNA corresponding to mouse Crispld2 (Myc-DDK-tagged;NM_030209) was obtained from OriGene Technologies, Inc. (MR222108) and subcloned into the EcoRI/NotI sites of pCDNA3 for transient transfections. Plasmid transient transfections
Showing 1-1 of 1 papers.
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