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Dynlrb1 (NM_025947) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR222083
Dynlrb1 (Myc-DDK-tagged) - Mouse dynein light chain roadblock-type 1 (Dynlrb1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Dynlrb1 or NM_025947"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dynlrb1
Synonyms 2010012N15Rik; 2010320M17Rik; 9430076K19Rik; AV124457; Dncl2a; DNLC2A; km23-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222083 representing NM_025947
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGGTGGAGGAAACACTCAAGAGGCTTCAGAGCCAGAAAGGAGTGCAGGGCATCATCGTGGTGA
ACACAGAAGGCATTCCCATCAAGAGCACAATGGACAATCCCACCACTACACAGTATGCCAACCTCATGCA
CAACTTCATCTTGAAGGCGCGGAGCACTGTGCGTGAGATTGACCCCCAAAACGACCTAACCTTCCTTCGA
ATTCGCTCCAAGAAAAATGAAATTATGGTGGCACCAGATAAAGACTATTTCCTGATTGTGATCCAGAATC
CAACTGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR222083 representing NM_025947
Red=Cloning site Green=Tags(s)

MAEVEETLKRLQSQKGVQGIIVVNTEGIPIKSTMDNPTTTQYANLMHNFILKARSTVREIDPQNDLTFLR
IRSKKNEIMVAPDKDYFLIVIQNPTE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025947
ORF Size 288 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025947.3, NP_080223.2
RefSeq Size 702 bp
RefSeq ORF 291 bp
Locus ID 67068
UniProt ID P62627
Cytogenetics 2 H1
MW 11.4 kDa
Summary Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.UniProtKB/Swiss-Prot Function
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"NM_025947" in other vectors (4)
SKU Description Size Price
MC210480 Dynlrb1 (untagged) - Mouse dynein light chain roadblock-type 1 (Dynlrb1), (10ug) 10 ug
$165.00
MG222083 Dynlrb1 (tGFP-tagged) - Mouse dynein light chain roadblock-type 1 (Dynlrb1), (10ug) 10 ug
$350.00
MR222083L3 Lenti ORF clone of Dynlrb1 (Myc-DDK-tagged) - Mouse dynein light chain roadblock-type 1 (Dynlrb1) 10 ug
$450.00
MR222083L4 Lenti ORF clone of Dynlrb1 (mGFP-tagged) - Mouse dynein light chain roadblock-type 1 (Dynlrb1) 10 ug
$450.00

Plasmid Map for MR222083

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR222083
Circular map for MR222083

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