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Rsph6a (NM_031255) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR222046
Rsph6a (Myc-DDK-tagged) - Mouse radial spoke head 6 homolog A (Chlamydomonas) (Rsph6a), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $649.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rsph6a
Synonyms Rshl1; RSP4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222046 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGAACCACCGCCCAATCCTGACCCCTCTCAAACCCGGAGGGCTTCCCAAGGCTCAGAAAGGGCAC
GGAGTCAAGAGTACTCTCAGCCTCTGTTAACCATCCCAGAGGATGGGTTGAACCGACCACCTCCGCAGCG
GGGCAGTCGGAGCAGTCAGGGCAGTCAGGACCTGCAGGGAACTGGTCTACCCCACTGGCCACAGAGGTCC
AGCCTGGTCCCAGATGTTCAAGGTGATGAAGGCACCGAGTACCACCAGTCCATGCCCTTAGGCTACACGC
CAGGCTTTCCCATGGAGTTCTCGCAACAGGGTTACCTGGATGATAGCAGGATGATGGAGCAGTTCCCACA
GGGCCAGGACCTCTTAGAACAACTGGAGTCTACCTACCAAGGCTCGGCTTCTGGAATCCTGGGCCAGCTC
AACTTGTATCCAAGAGAGGATGAGATATTCAGCCAGGACACACAGCACGGACCCTACCTGAGAGACGACC
CCTCCCTCCACCTGAGGCCCTCTGACCTGGGCTTCATGCCCATTGTTGGAGAGGTACCTGACCCCGAGCC
TCGTGAGCTGGCAATACAGAACGCCAAGGCCTACCTGCTGCGGACCAGCATGAGCTGCAACCTCAGCCTG
TATGAACACCTGGTGAACCTGCTGACCAAGATCCTGAACCAGCGCCCTGAGGACCCCTTGTCCATCCTGG
AGTCTCTGAACCGCACCATGCAGTGGGAATGGTTTCACCCCAAGCTGGACACGCTGCGGGATGACCCCGA
GATGCAGCCCACCTACGAGATGGCCGAAAAGCAAAAGGCTCTGTTTATCAGGGGTGGAGGAGAAGGCGAA
CAGGAAATGGAAGAGGAGGTGACTGACAGCCCGGTGCCCAACATCATGGAGACCGCCTTCTACTTCGAGC
AGGCGGGCGTGGGGCTGAGCTCGGACGAAAGCTTCCGAATCTTCCTGGCCCTGAAGCAGCTGGTGGAGCA
GCAGCCCATCCACATGTGCCGCTTCTGGGGCAAGATCCTGGGCCTGAGCCGCAGCTACCTGGTGGCTGAG
GTGGAGTTCCGCGAGGGCGAGGAGGAGGGAGAGGAGGAGGAGGTGGAGGAGATGATGGAGGGAGGCGAGG
TCTTGGAGACGCACGGTGAGGAGGAGGGAGAGGAGGATGAGGAGAAGGTGGTGGACAGCGTGCCCAAGCC
ACAGTGGAAGCCGCCGCCCATCATCCCCAAGGAGGAGAGCCGCAGTGGCACCAACAAATACCTGTACTTC
GTGTGCAACGAGCCAGGCCGCCCGTGGACGCGCCTGCCGCACGTGACGCCCGCGCAGATCGTGTGCGCGC
GCAAGATCAAAAAGTTCTTCACTGGCTTCCTGGACACGCCGGTCATCAGCTATCCGCCCTTCCCGGGCAA
CGAGGCCAACTATCTCCGCGCGCAGATTGCGCGCATCTCCGCCGCCACACACATCAGCCCGCTGGGCTTC
TACCAGTTCGGCGAGGAGGAGGGCGATGAGGAGGAGGAGGGCGGCGCGGGACGCGATTCCTTTGAGGAGA
ACCCCGACTTTGAGGGCATCCCCGTGTTGGAGCTCGTGGACTCCATGGCCAACTGGGTACATCACACTCA
GCACATCCTGCCTCAGGGGCGCTGTACATGGGTGAACCCACTGCAGAAAACAGAGGAGGAAGAAGAGCTA
GGGGAGGAGGAAGAGAAGGCAGACGAGGCAATGGAGGAGGTGGAGCAGGAGGTCGGTCCTCCGCTTCTGA
CTCCGCTCTCAGAAGATGCAGAAATCATGCACCTGTCGCCCTGGACCACCCGCCTCTCCTGCAGCCTCAG
CCCTCAGTACTCGGTGGCCATCGTGCGCTCCAACCTCTGGCCAGGGGCCTATGCCTACGCCACTGGCAAG
AAGTTTGAGAACATATACATTGGCTGGGGTCACAAGTATAGCCCCGAGAACTTCAACCCAATGCTGCCAG
CTCTGATTCAGCAAGAGTACCCCAGCGGCCCCGAAATCACGGAGATGAGTGACCCCACAGTGGAGGAGGA
GCAGGCCCTGAAGGCTGCTCAGGAGCAGGCCCTGGCAGCCGCTGAGGAAGAGGAGGAAGATGAGGAAGAG
GAGGAGGATGAAGACCTGGAGGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR222046 protein sequence
Red=Cloning site Green=Tags(s)

MGEPPPNPDPSQTRRASQGSERARSQEYSQPLLTIPEDGLNRPPPQRGSRSSQGSQDLQGTGLPHWPQRS
SLVPDVQGDEGTEYHQSMPLGYTPGFPMEFSQQGYLDDSRMMEQFPQGQDLLEQLESTYQGSASGILGQL
NLYPREDEIFSQDTQHGPYLRDDPSLHLRPSDLGFMPIVGEVPDPEPRELAIQNAKAYLLRTSMSCNLSL
YEHLVNLLTKILNQRPEDPLSILESLNRTMQWEWFHPKLDTLRDDPEMQPTYEMAEKQKALFIRGGGEGE
QEMEEEVTDSPVPNIMETAFYFEQAGVGLSSDESFRIFLALKQLVEQQPIHMCRFWGKILGLSRSYLVAE
VEFREGEEEGEEEEVEEMMEGGEVLETHGEEEGEEDEEKVVDSVPKPQWKPPPIIPKEESRSGTNKYLYF
VCNEPGRPWTRLPHVTPAQIVCARKIKKFFTGFLDTPVISYPPFPGNEANYLRAQIARISAATHISPLGF
YQFGEEEGDEEEEGGAGRDSFEENPDFEGIPVLELVDSMANWVHHTQHILPQGRCTWVNPLQKTEEEEEL
GEEEEKADEAMEEVEQEVGPPLLTPLSEDAEIMHLSPWTTRLSCSLSPQYSVAIVRSNLWPGAYAYATGK
KFENIYIGWGHKYSPENFNPMLPALIQQEYPSGPEITEMSDPTVEEEQALKAAQEQALAAAEEEEEDEEE
EEDEDLED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_031255
ORF Size 2124 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031255.1, NM_031255.2, NP_112545.2
RefSeq Size 2357 bp
RefSeq ORF 2127 bp
Locus ID 83434
UniProt ID Q8CDR2
Cytogenetics 7 A3
MW 80.2 kDa
Summary Essential for sperm flagellar assembly and male fertility.UniProtKB/Swiss-Prot Function
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Documents
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"NM_031255" in other vectors (4)
SKU Description Size Price
MC220864 Rsph6a (untagged) - Mouse radial spoke head 6 homolog A (Chlamydomonas) (Rsph6a), transcript variant 1, (10ug) 10 ug
$714.00
MG222046 Rsph6a (tGFP-tagged) - Mouse radial spoke head 6 homolog A (Chlamydomonas) (Rsph6a) transcript variant 1, (10ug) 10 ug
$849.00
MR222046L3 Lenti ORF clone of Rsph6a (Myc-DDK-tagged) - Mouse radial spoke head 6 homolog A (Chlamydomonas) (Rsph6a), transcript variant 1 10 ug
$949.00
MR222046L4 Lenti ORF clone of Rsph6a (mGFP-tagged) - Mouse radial spoke head 6 homolog A (Chlamydomonas) (Rsph6a), transcript variant 1 10 ug
$949.00

Plasmid Map for MR222046

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR222046
Circular map for MR222046

file-alt Citations

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