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Pirt (NM_178656) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR222009
Pirt (Myc-DDK-tagged) - Mouse phosphoinositide-interacting regulator of transient receptor potential channels (Pirt)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Pirt or NM_178656"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pirt
Synonyms A530088H08Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222009 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTGCTCCCCAAGGCCCTGGAGGTAGACGAGAGGTCTCCAGAGTCCAAGGACCTGCTGCCCAGCC
AGACAGCCAGCTCCCTGTGCATCAGTTCCAGAAGTGAGTCTGTCTGGACCACCACACCCAAAAGCAACTG
GGAAATCTACCACAAGCCCATCATCATCATGTCAGTGGGAGCTGCCATTCTGCTCTTTGGCGTGGCCATC
ACCTGTGTGGCCTACATCTTGGAAGAGAAGCATAAAGTTGTGCAAGTGCTCAGGATGATAGGGCCTGCCT
TCCTGTCCCTGGGACTCATGATGCTGGTGTGTGGGCTGGTGTGGGTCCCCATAATCAAAAAGAAGCAGAA
GCAAAGGCAGAAGTCCAACTTCTTCCAAAGCCTCAAGTTCTTCCTCCTGAACCGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR222009 protein sequence
Red=Cloning site Green=Tags(s)

MEVLPKALEVDERSPESKDLLPSQTASSLCISSRSESVWTTTPKSNWEIYHKPIIIMSVGAAILLFGVAI
TCVAYILEEKHKVVQVLRMIGPAFLSLGLMMLVCGLVWVPIIKKKQKQRQKSNFFQSLKFFLLNR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178656
ORF Size 405 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178656.3, NP_848771.1
RefSeq Size 3014 bp
RefSeq ORF 408 bp
Locus ID 193003
UniProt ID Q8BFY0
Cytogenetics 11 B3
MW 15.3 kDa
Summary Regulatory subunit of TRPV1, a molecular sensor of noxious heat and capsaicin. Positively regulates TRPV1 channel activity via phosphatidylinositol 4,5-bisphosphate (PIP2). Binds various phosphoinositide, including phosphatidylinositol 4,5-bisphosphate (PIP2), but not phosphatidylinositol (PI).UniProtKB/Swiss-Prot Function
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"NM_178656" in other vectors (4)
SKU Description Size Price
MC212451 Pirt (untagged) - Mouse phosphoinositide-interacting regulator of transient receptor potential channels (Pirt), (10ug) 10 ug
$150.00
MG222009 Pirt (tGFP-tagged) - Mouse phosphoinositide-interacting regulator of transient receptor potential channels (Pirt), (10ug) 10 ug
$350.00
MR222009L3 Lenti ORF clone of Pirt (Myc-DDK-tagged) - Mouse phosphoinositide-interacting regulator of transient receptor potential channels (Pirt) 10 ug
$450.00
MR222009L4 Lenti ORF clone of Pirt (mGFP-tagged) - Mouse phosphoinositide-interacting regulator of transient receptor potential channels (Pirt) 10 ug
$450.00

Plasmid Map for MR222009

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR222009
Circular map for MR222009

file-alt Citations

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