Art2a-ps (NM_007490) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR222005
Art2a (Myc-DDK-tagged) - Mouse ADP-ribosyltransferase 2a, pseudogene (Art2a-ps), transcript variant 1
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Art2a-ps
Synonyms Art2a; ARTC2; Ly92a; Rt-6; Rt6; Rt6-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222005 representing NM_007490
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCATCAAATAATTTCAAGTTCTTCCTAACTTGGTGGCTAACCCAGCAGGTGACTGGCCTTGCAGTGC
CTTTCATGCTAGACATGGCTCCAAATGCATTTGATGATCAGTATGAGGGCTGTGTCGAAGACATGGAGAA
AAAGGCACCCCAGCTGTTACAAGAAGACTTCAACATGAATGAGGAATTAAAACTTGAGTGGGAAAAAGCA
GAGATAAAATGGAAGGAGATCAAAAATTGTATGAGTTATCCGGCAGGTTTCCATGATTTCCATGGAACAG
CTTTAGTTGCCTACACTGGGAACATCCACAGAAGCCTTAATGAGGCTACTAGAGAGTTCAAAATAAATCC
TGGTAACTTCCACTACAAGGCCTTCCATTACTACTTAACAAGAGCTCTTCAGCTTTTGAGTGACCAGGGT
TGTCGTTCAGTTTACCGAGGTACTAATGTCAGGTTTCGTTACACTGGGAAGGGCTCTGTGCGATTTGGGC
ATTTCGCATCCTCTTCTTTAAACCGGAGTGTAGCTACTTCTAGTCCATTTTTCAATGGACAGGGGACATT
ATTTATCATCAAAACCTGCTTGGGGGCTCACATCAAACATTGTTCCTACTATACTCATGAAGAGGAGGTG
TTAATTCCAGGCTATGAAGTATTTCACAAAGTCAAAACACAAAGTGTCGAACGGTATATCCAAATTTCTC
TGGACTCCCCAAAAAGGAAGAAGAGCAACTTTAATTGCTTCTATAGCGGTTCTACTCAAGCAGCCAACGT
TAGCAGCTTAGGATCTAGAGAGAGCTGTGTACCCCTGTTCCTTGTGGTTCTCCTCGGTCTTCTGGTCCAG
CAGCTTACTCTTGCTGAGCCG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR222005 representing NM_007490
Red=Cloning site Green=Tags(s)

MPSNNFKFFLTWWLTQQVTGLAVPFMLDMAPNAFDDQYEGCVEDMEKKAPQLLQEDFNMNEELKLEWEKA
EIKWKEIKNCMSYPAGFHDFHGTALVAYTGNIHRSLNEATREFKINPGNFHYKAFHYYLTRALQLLSDQG
CRSVYRGTNVRFRYTGKGSVRFGHFASSSLNRSVATSSPFFNGQGTLFIIKTCLGAHIKHCSYYTHEEEV
LIPGYEVFHKVKTQSVERYIQISLDSPKRKKSNFNCFYSGSTQAANVSSLGSRESCVPLFLVVLLGLLVQ
QLTLAEP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007490
ORF Size 861 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007490.1, NP_031516.1
RefSeq Size 864 bp
RefSeq ORF 864 bp
Locus ID 11871
UniProt ID P17981
Cytogenetics 7 54.6 cM
MW 32.8 kDa
Summary Has both ADP-ribosyltransferase activity and thiol-dependent NAD(+) glycohydrolase activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_007490" in other vectors (4)
SKU Description Size Price
MC208154 Art2a (untagged) - Mouse ADP-ribosyltransferase 2a, pseudogene (Art2a-ps), transcript variant 1, (10ug) 10 ug
$330.00
MG222005 Art2a (tGFP-tagged) - Mouse ADP-ribosyltransferase 2a pseudogene (Art2a-ps) transcript variant 1, (10ug) 10 ug
$500.00
MR222005L3 Lenti ORF clone of Art2a (Myc-DDK-tagged) - Mouse ADP-ribosyltransferase 2a, pseudogene (Art2a-ps), transcript variant 1 10 ug
$600.00
MR222005L4 Lenti ORF clone of Art2a (mGFP-tagged) - Mouse ADP-ribosyltransferase 2a, pseudogene (Art2a-ps), transcript variant 1 10 ug
$600.00

Plasmid Map for MR222005

Download sequence for

Cloning scheme for MR222005

Circular map for MR222005

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