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Nfatc2ip (NM_010900) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR221981
Nfatc2ip (Myc-DDK-tagged) - Mouse nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein (Nfatc2ip)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Nfatc2ip or NM_010900"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nfatc2ip
Synonyms D7Ertd304e; NIP45
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR221981 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAACCACTGAGGGGACGTGGTCCGAGGTCCCGCGGTGGCCGAGGCGCTCGGAGAGCCCGAGGCG
CCCGTGGCCGGTGTCCTCGCGCCCGGCAGTCTCCGGCTAGGCTCATTCCAGACACCGTGCTTGTGGACTT
GGTCAGTGACAGCGACGAAGAGGTCTTGGAAGTCGCAGACCCAGTAGAGGTGCCGGTCGCCCGCCTCCCC
GCGCCGGCTAAACCTGAGCAGGACAGCGACAGTGACAGTGAAGGGGCGGCCGAGGGGCCTGCGGGAGCCC
CGCGTACATTGGTGCGACGGCGGCGGCGGCGGCTGCTGGATCCCGGAGAGGCGCCGGTGGTCCCAGTGTA
CTCCGGGAAGGTACAGAGCAGCCTCAACCTCATTCCAGATAATTCATCCCTCTTGAAACTGTGCCCTTCA
GAGCCTGAAGATGAGGCAGATCTGACAAATTCTGGCAGTTCTCCCTCTGAGGATGATGCCCTGCCTTCAG
GTTCTCCCTGGAGAAAGAAGCTCAGAAAGAAGTGTGAGAAAGAAGAAAAGAAAATGGAAGAGTTTCCGGA
CCAGGACATCTCTCCTTTGCCCCAACCTTCGTCAAGGAACAAAAGCAGAAAGCATACGGAGGCGCTCCAG
AAGCTAAGGGAAGTGAACAAGCGTCTCCAAGATCTCCGCTCCTGCCTGAGCCCCAAGCAGCACCAGAGTC
CAGCCCTTCAGAGCACAGATGATGAGGTGGTCCTAGTGGAAGGGCCTGTCTTGCCACAGAGCTCTCGACT
CTTTACACTCAAGATCCGGTGCCGGGCTGACCTAGTGAGACTGCCTGTCAGGATGTCGGAGCCCCTTCAG
AATGTGGTGGATCACATGGCCAATCATCTTGGGGTGTCTCCAAACAGGATTCTTTTGCTTTTTGGAGAGA
GTGAACTGTCTCCTACTGCCACCCCTAGTACCCTAAAGCTTGGAGTGGCTGACATCATTGATTGTGTGGT
GCTAGCAAGCTCTTCAGAGGCCACAGAGACATCCCAGGAGCTCCGGCTCCGGGTGCAGGGGAAGGAGAAA
CACCAGATGTTGGAGATCTCACTGTCTCCTGATTCTCCTCTTAAGGTTCTCATGTCACACTATGAGGAAG
CCATGGGACTCTCTGGACACAAGCTCTCCTTCTTCTTTGATGGGACAAAGCTTTCAGGCAAGGAGCTGCC
AGCTGATCTGGGCCTGGAATCCGGAGATCTCATCGAAGTCTGGGGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR221981 protein sequence
Red=Cloning site Green=Tags(s)

MAEPLRGRGPRSRGGRGARRARGARGRCPRARQSPARLIPDTVLVDLVSDSDEEVLEVADPVEVPVARLP
APAKPEQDSDSDSEGAAEGPAGAPRTLVRRRRRRLLDPGEAPVVPVYSGKVQSSLNLIPDNSSLLKLCPS
EPEDEADLTNSGSSPSEDDALPSGSPWRKKLRKKCEKEEKKMEEFPDQDISPLPQPSSRNKSRKHTEALQ
KLREVNKRLQDLRSCLSPKQHQSPALQSTDDEVVLVEGPVLPQSSRLFTLKIRCRADLVRLPVRMSEPLQ
NVVDHMANHLGVSPNRILLLFGESELSPTATPSTLKLGVADIIDCVVLASSSEATETSQELRLRVQGKEK
HQMLEISLSPDSPLKVLMSHYEEAMGLSGHKLSFFFDGTKLSGKELPADLGLESGDLIEVWG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010900
ORF Size 1236 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010900.3, NP_035030.2
RefSeq Size 3388 bp
RefSeq ORF 1239 bp
Locus ID 18020
UniProt ID O09130
Cytogenetics 7 69.01 cM
MW 45.1 kDa
Summary In T-helper 2 (Th2) cells, regulates the magnitude of NFAT-driven transcription of a specific subset of cytokine genes, including IL3, IL4, IL5 and IL13, but not IL2. Recruits PRMT1 to the IL4 promoter; this leads to enhancement of histone H4 'Arg-3'-methylation and facilitates subsequent histone acetylation at the IL4 locus, thus promotes robust cytokine expression. Down-regulates formation of poly-SUMO chains by UBE2I/UBC9.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010900" in other vectors (4)
SKU Description Size Price
MC209024 Nfatc2ip (untagged) - Mouse nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein (Nfatc2ip), (10ug) 10 ug
$503.00
MG221981 Nfatc2ip (tGFP-tagged) - Mouse nuclear factor of activated T-cells cytoplasmic calcineurin-dependent 2 interacting protein (Nfatc2ip), (10ug) 10 ug
$657.00
MR221981L3 Lenti ORF clone of Nfatc2ip (Myc-DDK-tagged) - Mouse nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein (Nfatc2ip) 10 ug
$757.00
MR221981L4 Lenti ORF clone of Nfatc2ip (mGFP-tagged) - Mouse nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein (Nfatc2ip) 10 ug
$757.00

Plasmid Map for MR221981

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR221981
Circular map for MR221981

file-alt Citations

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