Foxc2 (NM_013519) Mouse Tagged ORF Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Foxc2 |
| Synonyms | Fkh14; Hfhbf3; MFH-1; Mfh1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_013519 |
| ORF Size | 1482 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_013519.2, NP_038547.2 |
| RefSeq Size | 2739 bp |
| RefSeq ORF | 1485 bp |
| Locus ID | 14234 |
| UniProt ID | Q61850 |
| Cytogenetics | 8 70.33 cM |
| MW | 53.3 kDa |
| Summary | Transcriptional activator. Might be involved in the formation of special mesenchymal tissues.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_013519" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC216796 | Foxc2 (untagged) - Mouse forkhead box C2 (Foxc2), (10ug) | 10 ug |
$732.00
|
|
| MG221977 | Foxc2 (tGFP-tagged) - Mouse forkhead box C2 (Foxc2), (10ug) | 10 ug |
$886.00
|
|
| MR221977L3 | Lenti ORF clone of Foxc2 (Myc-DDK-tagged) - Mouse forkhead box C2 (Foxc2) | 10 ug |
$986.00
|
|
| MR221977L4 | Lenti ORF clone of Foxc2 (mGFP-tagged) - Mouse forkhead box C2 (Foxc2) | 10 ug |
$986.00
|
|
Citations
Nanochannel-Based Poration Drives Benign and Effective Nonviral Gene Delivery to Peripheral Nerve Tissue
Moore, Wier, Lemmerman et al
Adv Biosyst (2020)
Moore, Wier, Lemmerman et al
Adv Biosyst (2020)
Cited by 24
AI Summary: OriGene's fluorescently labeled plasmid DNA (PCMV6) was used as a model genetic cargo to evaluate the effectiveness of nanochannel-based tissue nanotransfection for nonviral gene delivery to peripheral nerve tissue in this study.
Snippet: To examine whether TNT can effectively deliver genetic cargo to peripheral nerves, we proceeded to conduct gene delivery experiments in the sciatic nerve of 8-10‐week‐old C57BL/6 mice using fluorescently labeled plasmid DNA (PCMV6, 4.9 kb, Origene) as model
Early Intervention in Ischemic Tissue with Oxygen Nanocarriers Enables Successful Implementation of Restorative Cell Therapies
Diaz-Starokozheva, Das, Gu et al
Cel. Mol. Bioeng. (2020)
Diaz-Starokozheva, Das, Gu et al
Cel. Mol. Bioeng. (2020)
Cited by 16
Induction of GDNF and GFRα-1 Following AAV1-Rheb(S16H) Administration in the Hippocampus in vivo
Yun, Jeon, Kim et al
Exp Neurobiol (2020) 29 (2), 164-175 DOI: 10.5607/en19075
Yun, Jeon, Kim et al
Exp Neurobiol (2020) 29 (2), 164-175 DOI: 10.5607/en19075
Cited by 16
AI Summary: OriGene’s Rheb plasmid was used as the template for generating the constitutively active Rheb(S16H) construct, enabling the study of its effects on neurotrophic factor expression and neuroprotection in the rat hippocampus.
Snippet: … A plasmid carrying Rheb was purchased from OriGene Technologies (Rockville, MD, USA). Rheb DNA was amplified and modified to incorporate a FLAG-encoding sequence at the 3'-end by expanded long-template PCR (Roche, Basel, Switzerland). Rheb(S16H) was generated with the Phusion Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA, USA) in the pGEM-T vector (Promega, Madison, WI, USA), and then cloned into an AAV packaging construct that utilizes the chicken β-actin promoter and contains a 3' WPRE (pBL). AAVs were produced by the University of North Carolina Vector Core, and the genomic titer of Rheb(S16H) was 2×1012 viral genomes/ml. …
Showing 1-3 of 3 papers.
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