Foxc2 (NM_013519) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG221977
Foxc2 (tGFP-tagged) - Mouse forkhead box C2 (Foxc2), (10ug)
  $886.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Foxc2
Synonyms Fkh14; Hfhbf3; MFH-1; Mfh1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG221977 representing NM_013519
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGCGCGTTACTCGGTATCGGACCCCAACGCCCTGGGAGTGGTACCCTATTTGAGTGAGCAAAACT
ACTACCGGGCGGCCGGCAGCTACGGCGGCATGGCCAGCCCCATGGGCGTCTACTCCGGCCACCCGGAGCA
GTACGGCGCCGGCATGGGCCGCTCCTACGCGCCCTACCACCACCAGCCCGCGGCGCCCAAGGACCTGGTG
AAGCCGCCCTACAGCTATATAGCGCTCATCACCATGGCGATCCAGAACGCGCCAGAGAAGAAGATCACTC
TGAACGGCATCTACCAGTTCATCATGGACCGTTTCCCCTTCTACCGCGAGAACAAGCAGGGCTGGCAGAA
CAGCATCCGCCACAACCTGTCACTCAATGAGTGCTTCGTGAAAGTGCCGCGCGACGACAAGAAGCCGGGC
AAGGGCAGCTACTGGACGCTCGACCCGGACTCCTACAACATGTTCGAGAATGGCAGCTTCCTGCGGCGGC
GGCGGCGCTTCAAGAAGAAGGATGTGCCCAAGGACAAGGAGGAGCGGGCCCACCTCAAGGAGCCGCCCTC
GACCACGGCCAAGGGCGCTCCGACAGGGACCCCGGTAGCTGACGGGCCCAAGGAGGCCGAGAAGAAAGTC
GTGGTTAAGAGCGAGGCGGCGTCCCCCGCGCTGCCGGTCATCACCAAGGTGGAGACGCTGAGCCCCGAGG
GAGCGCTGCAGGCCAGTCCGCGCAGCGCATCCTCCACGCCCGCAGGTTCCCCAGACGGCTCGCTGCCGGA
GCACCACGCCGCGGCGCCTAACGGGCTGCCCGGCTTCAGCGTGGAGACCATCATGACGCTGCGCACGTCG
CCTCCGGGCGGCGATCTGAGCCCAGCGGCCGCGCGCGCCGGCCTGGTGGTGCCACCGCTGGCACTGCCAT
ACGCCGCAGCGCCACCCGCCGCTTACACGCAGCCGTGCGCGCAGGGCCTGGAGGCTGCGGGCTCCGCGGG
CTACCAGTGCAGTATGCGGGCTATGAGTCTGTACACCGGGGCCGAGCGGCCCGCGCACGTGTGCGTTCCG
CCCGCGCTGGACGAGGCTCTGTCGGACCACCCGAGCGGCCCCGGCTCCCCGCTCGGCGCCCTCAACCTCG
CAGCGGGTCAGGAGGGCGCGTTGGGGGCCTCGGGTCACCACCACCAGCATCACGGCCACCTCCACCCGCA
GGCGCCACCGCCCGCCCCGCAGCCCCCTCCCGCGCCGCAGCCCGCCACCCAGGCCACCTCCTGGTATCTG
AACCACGGCGGGGACCTGAGCCACCTCCCCGGCCACACGTTTGCAACCCAACAGCAAACTTTCCCCAACG
TCCGGGAGATGTTCAACTCGCACCGGCTAGGACTGGACAACTCGTCCCTCGGGGAGTCCCAGGTGAGCAA
TGCGAGCTGTCAGCTGCCCTATCGAGCTACGCCGTCCCTCTACCGCCACGCAGCCCCCTACTCTTACGAC
TGCACCAAATAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG221977 representing NM_013519
Red=Cloning site Green=Tags(s)

MQARYSVSDPNALGVVPYLSEQNYYRAAGSYGGMASPMGVYSGHPEQYGAGMGRSYAPYHHQPAAPKDLV
KPPYSYIALITMAIQNAPEKKITLNGIYQFIMDRFPFYRENKQGWQNSIRHNLSLNECFVKVPRDDKKPG
KGSYWTLDPDSYNMFENGSFLRRRRRFKKKDVPKDKEERAHLKEPPSTTAKGAPTGTPVADGPKEAEKKV
VVKSEAASPALPVITKVETLSPEGALQASPRSASSTPAGSPDGSLPEHHAAAPNGLPGFSVETIMTLRTS
PPGGDLSPAAARAGLVVPPLALPYAAAPPAAYTQPCAQGLEAAGSAGYQCSMRAMSLYTGAERPAHVCVP
PALDEALSDHPSGPGSPLGALNLAAGQEGALGASGHHHQHHGHLHPQAPPPAPQPPPAPQPATQATSWYL
NHGGDLSHLPGHTFATQQQTFPNVREMFNSHRLGLDNSSLGESQVSNASCQLPYRATPSLYRHAAPYSYD
CTKY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013519
ORF Size 1482 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013519.2, NP_038547.2
RefSeq Size 2739 bp
RefSeq ORF 1485 bp
Locus ID 14234
UniProt ID Q61850
Cytogenetics 8 70.33 cM
Summary Transcriptional activator. Might be involved in the formation of special mesenchymal tissues.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013519" in other vectors (4)
SKU Description Size Price
MC216796 Foxc2 (untagged) - Mouse forkhead box C2 (Foxc2), (10ug) 10 ug
$732.00
MR221977 Foxc2 (Myc-DDK-tagged) - Mouse forkhead box C2 (Foxc2) 10 ug
$686.00
MR221977L3 Lenti ORF clone of Foxc2 (Myc-DDK-tagged) - Mouse forkhead box C2 (Foxc2) 10 ug
$986.00
MR221977L4 Lenti ORF clone of Foxc2 (mGFP-tagged) - Mouse forkhead box C2 (Foxc2) 10 ug
$986.00

Plasmid Map for MG221977

Download sequence for

Cloning scheme for MG221977

Circular map for MG221977

file-alt Citations

Nanochannel-Based Poration Drives Benign and Effective Nonviral Gene Delivery to Peripheral Nerve Tissue
Moore, Wier, Lemmerman et al
Adv Biosyst (2020)
Cited by 24
AI Summary: OriGene's fluorescently labeled plasmid DNA (PCMV6) was used as a model genetic cargo to evaluate the effectiveness of nanochannel-based tissue nanotransfection for nonviral gene delivery to peripheral nerve tissue in this study.
Snippet: To examine whether TNT can effectively deliver genetic cargo to peripheral nerves, we proceeded to conduct gene delivery experiments in the sciatic nerve of 8-10‐week‐old C57BL/6 mice using fluorescently labeled plasmid DNA (PCMV6, 4.9 kb, Origene) as model
Showing 1-1 of 1 papers.
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