Pnn (NM_008891) Mouse Tagged ORF Clone

SKU MR221940

Pnn (Myc-DDK-tagged) - Mouse pinin (Pnn)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Pnn
• Synonyms: AU045199; D12Ertd512e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221940 representing NM_008891; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGTCGCCGTGAGAGCTTTGCAGGAGCAGCTAGAGAAGGCCAAAGAGAGCCTCAAGAATGTGGACG; AGAATATCCGCAAGCTCACCGGGCGGGACCCGAATGATGTGAGGCCCATCCAAGCCAGATTGCTGGCCCT; TTCTGGTCCTGGTGGAGGTAGAGGACGAGGTAGCTTATTGCTAAGGCGTGGATTCTCAGATAGTGGAGGA; GGACCCCCAGCCAAACAAAGAGACCTGGAAGGGGCAGTCAGTAGGCTGGGTGGGGAGCGTCGGACAAGAA; GAGAATCACGCCAGGAGAGCGACCCAGAAGACGATGATGTTAAAAAGCCAGCACTGCAATCTTCCGTTGT; AGCTACCTCCAAAGAGCGCACGCGCAGAGACCTTATCCAGGATCAAAACATGGATGAGAAGGGGAAGCAA; AGGAACCGACGAATATTTGGCTTGTTGATGGGCACTCTTCAGAAATTTAAACAAGAATCTACTGTTGCTA; CTGAACGGCAAAAAAGACGCCAAGAAATTGAACAAAAACTTGAAGTTCAGGCTGAAGAAGAGAGAAAGCA; GGTTGAAAACGAGAGGCGAGAACTGTTTGAGGAGAGACGTGCTAAGCAGACGGAGCTGCGGCTTCTGGAG; CAGAAGGTTGAGCTTGCGCAGCTGCAAGAAGAATGGAATGAACATAATGCCAAGATAATTAAGTATATAA; GGACCAAGACAAAGCCCCATTTGTTCTATATTCCTGGAAGAATGTGTCCAGCTACCCAAAAGCTAATAGA; AGAGTCACAGAGAAAGATGAATGCTTTATTTGAAGGTAGACGCATCGAATTTGCAGAACAAATAAATAAA; ATGGAGGCTAGGCCTAGAAGACAATCAATGAAGGAAAAAGAGCATCAGGTGGTGCGTAATGAAGAACAGA; AGGCGGAACAAGAAGAGGGTAAGGTGGCTCAGCGAGAGGAAGAGTTGGAGGAGACAGGTAATCAGCACAA; TGATGTAGAAGTAGAGGAGGCAGGAGAGGAAGAGGAGAAGGAAGCAGGTATAGTTCATAGTGATGCAGAG; AAAGAACAGGAGGAGGAGGAACAAAAGCAGGAAATGGAGGTTAAGACTGAGGAGGAGGCTGAGGTAAGAG; AAGGTGAGAAGCAGCAGGATAGTCAGCCAGAAGAAGTGATGGATGTGCTAGAGATGGTGGAAAGTGTCAA; ACACGTAATTGCTGAGCAGGAAGTAATGGAAACTAACCAAGTTGAAAGTATAGAGCCTTCAGAAAATGAA; ACTAGCAAAGAATTGGAGCCAGAGATGGAATTTGATGTTGAGCCAGATAAAGAATGTAAATCTCTTTCTC; CTGGAAAAGAGAATATTAATTCTCAGGAAGTGGAAAAGGAGTCTGAGGAGAAAGAGGAAAAAGAAGAGAA; AGAACCTGAGCCCCAACCTGAGCCTGTGGCTCAGCCTCAGCCCCCACCTCAGCCCCTGCCCCAGAGCCAG; CCCCATTCTCAGCCCCACTCCCAGCCCCAGCCTGTACTCCAGTCCCAGCCTCTCTGTCAGCCTGAGACTT; TGCCATTAGCTGTTTTGCAGCCTCCACCTCAGGTTATTCAGGAGCAAGGGAATCTACTACCTGAGCGGAA; GGATTTTCCTCTAGAGTCTATAAAACTCCCTGAGGTGTCTGTAGAGCCAGTCTTGACAGTACATTCAGAA; AACAAAAGCAAGAATAAGACTAGGAGCAGGAGCAGGGGGCGAGCAAGGAATAAAACAAGCAAGAGTAGAA; GTCGGAGCAGCAGCAGCAGTAGTTCCAGTAGCAGCTCTACTAGTAGCAGCAGCGGGAGCAGCTCCAGCAG; TGGCAGCAGCAGTAGTCGAAGCAGCTCCAGCAGCAGCTCCAGCACGAGTGGGAGCAGCAGCAGAGACAGC; AGCAGCAGCACTAGCAGTAGCAGTGAGAGTAGGAGTCGCAGTAGGGGCCGAGGACATAACCGAGATAGGA; AGCACAGAAGGAGCGTGGACCGGAAGCGAAGGGATACTTCAGGACTAGAAAGAAGTCACAAGTCTTCAAA; AGGTGGTAGTAGTAGAGATACAAAAGGATCAAAGGATAAGAGTTCCCGCCCTGACAGAAAAAGGTCTATA; TCAGAGAGTAGTCGATCAGGCAAAAGATCTTCAAGAAGTGAAAGAGACCGAAAATCAGACAGGAAAGACA; AAAGGCGT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221940 representing NM_008891; Red=Cloning site Green=Tags(s); ; MAVAVRALQEQLEKAKESLKNVDENIRKLTGRDPNDVRPIQARLLALSGPGGGRGRGSLLLRRGFSDSGG; GPPAKQRDLEGAVSRLGGERRTRRESRQESDPEDDDVKKPALQSSVVATSKERTRRDLIQDQNMDEKGKQ; RNRRIFGLLMGTLQKFKQESTVATERQKRRQEIEQKLEVQAEEERKQVENERRELFEERRAKQTELRLLE; QKVELAQLQEEWNEHNAKIIKYIRTKTKPHLFYIPGRMCPATQKLIEESQRKMNALFEGRRIEFAEQINK; MEARPRRQSMKEKEHQVVRNEEQKAEQEEGKVAQREEELEETGNQHNDVEVEEAGEEEEKEAGIVHSDAE; KEQEEEEQKQEMEVKTEEEAEVREGEKQQDSQPEEVMDVLEMVESVKHVIAEQEVMETNQVESIEPSENE; TSKELEPEMEFDVEPDKECKSLSPGKENINSQEVEKESEEKEEKEEKEPEPQPEPVAQPQPPPQPLPQSQ; PHSQPHSQPQPVLQSQPLCQPETLPLAVLQPPPQVIQEQGNLLPERKDFPLESIKLPEVSVEPVLTVHSE; NKSKNKTRSRSRGRARNKTSKSRSRSSSSSSSSSSSTSSSSGSSSSSGSSSSRSSSSSSSSTSGSSSRDS; SSSTSSSSESRSRSRGRGHNRDRKHRRSVDRKRRDTSGLERSHKSSKGGSSRDTKGSKDKSSRPDRKRSI; SESSRSGKRSSRSERDRKSDRKDKRR; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008891
• ORF Size: 2178 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_008891.2, NP_032917.2
• RefSeq Size: 3469 bp
• RefSeq ORF: 2181 bp
• Locus ID: 18949
• UniProt ID: O35691
• Cytogenetics: 12 26.0 cM
• MW: 83 kDa
• Summary: Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. Involved in the establishment and maintenance of epithelia cell-cell adhesion (By similarity).[UniProtKB/Swiss-Prot Function]