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Bbs10 (NM_027914) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR221925
Bbs10 (Myc-DDK-tagged) - Mouse Bardet-Biedl syndrome 10 (human) (Bbs10)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $653.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bbs10
Synonyms 1300007O09Rik; AI452285
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR221925 representing NM_027914
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCCCAGGGGTCGGTGACGGCGGCTTTGCGGGTGGCCGAGGTGCTGGAGAGCATCGCGAACCGCT
GCGTGGGGCCCGAGGGCGGCCAAGTGTTGTGTACGAAGCCCACCGGCGAGGTGCTGCTTAGCAGGGATGG
AGGCTGCCTCCTGGAGGCGCTGCACTTAGAGCATCCCTTAGCCAGGATGATAGTGGCGTGTGTTTCCAGT
CACCTTAAAAAGACAGGAGATGGTGCTAAGACATTTATTATTTTTCTTTGCCATTTACTCAGAGGACTTC
ATGCAATCGGAGAAAAAGGAAAAGATTCATTCACTTCTGAAAATATTCAGAGCCATGAAAGGCATTGGAA
AAACTGTTGTCAGTGGAAATCTATTTCCCAGGCTCTGCAAACGTTTCAGACACAAACCCTAGGTTGTATT
GTAGACCGGAGTTTAAGCAGGCACTACCTGTCTGTCTTTTCTTCATCCACTGAAGGAAGAAAACTGTGCA
GGCACTCGCTAGAGTTGCTCCTGGAAGCATACTTTTGTGGAAGAGTGGGAAGAAACAATCACAGATTCAT
TTCACAGTTGATGTGTGACTACGTTTTCAAGTGTATGGCTTGTGAAAGTGGGGTTGAAGTATTTGAGTTG
TTGGATCACTGTTTTGCAGAGTTGAATGTTGGCGTGACAGGCCTTCCTGTTTCAGATTCTAGAATCATAG
ATGGCCTTGTGCTTCCCAGGGACTTTTCTATGTACTGCCCAGCAGATGGTGACATTAGGATGGTGATAGT
AACAGAAATCCTCCAGCCTCAGTTTTCATCGGCTGGCTCGGAGTTTGTTCTCAATTCAGAAACACAGTTT
CAGGCATCTCAGTGCTGGATCACGGACAGGACAAAAACAGTAATGAACCACTTGCGTGGTCAGAATGTAA
AACTGCTCCTGACCAGTGTGAAGCAACCAGACCTGGTTATTTACTGTGCAAGACTTAATAGCATATCAGT
GGTGGAGTGTTTATCAGCCGAAGAAGTTTCCCTTGTCCAGAGGATCACTGGTCTTTCTCCTTGTGTCCTA
CCAGAGGTTGCTTCACAGTGTGAAATTTCTGATAGTACTTTGGTGAAGTTTTGCAAGCCCCTCATCCTTA
GATCCAAGAGGTATGTTCATCTCGGCTTGATTAGCACATGTGCATTTATACCTCACTCTATGGTCCTCTG
TGGACCGGTTCTGGGTCTTGTTGAACAGCATGAGAGAGCTTTTCACGGAGCATTTAAGATGCTTCGGCAG
CTATTTACTGACCTTGATCTAAATTACATCATACAAACCAAACAGCAATGTAACCCAAGTCCTCTTGCTT
ATGACAATAGCAGAGAGCGTAATCACTCACCAGAAACTGATAAGTATCAGGACATAGTAGCAAAGAGCAA
AAATAAATTGGAAACTCAAACACATTTAGAAGTATATTCAGGTTTGGGGGCTTCAGATACAGAATTGAGA
GCAGGTAAGCCGTGGTCAGCACACAAGAAGACACCAATAGCTCCATCCCAAACAGATGAAATGCTGAAGT
GCTTGCCTCCAGAAAGAAGCGGGATAATTGATAACTGTGACCTGTCAATTGAGAATCATTCCACTGGAAA
TCCTACAGCAGAAGACACTGGAACAGAAATTTCTTTCGAACACTTACAGGTCTCAGATAATGCCGGAAAG
GGTTACACGCTGCCAGTGATGCGCAAGTCACTAGATACCTGTACTTGTCAGGGTTATTGTTCATCCACTG
TGCCAGCAGGCTGTGTGCTGCCAGTGGGTGGAAGTTTTGAGATTTTGATGAGTTACTACCTTCTCAGCTA
TGCCAAACAATGCCGACAATCAGATGAAACTGTCATCAGTATGTTAATAGCTGATGCTCTTCTAGGCATC
CCTAAAATCCTTTATAAGCCTAAGAAGGGAAAGGACAGTTTCCCACACATATACATGCGGTCTCTCCATG
CACTGCAAGCCAGTCAACCCATGGTAAGTGGTCAATCAGGGTTCGAGTCAGTAGCTGGTAAGTACCAATT
ACTAACTTCAGTTCTTCAGTGTTTGATGAAAATATTAACCATTGATTTAATAATCAACATCAAGAGGCAA
CCTCAGAAAACTGCTGACCAAGAATCAGAAGACGAATTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR221925 representing NM_027914
Red=Cloning site Green=Tags(s)

MASQGSVTAALRVAEVLESIANRCVGPEGGQVLCTKPTGEVLLSRDGGCLLEALHLEHPLARMIVACVSS
HLKKTGDGAKTFIIFLCHLLRGLHAIGEKGKDSFTSENIQSHERHWKNCCQWKSISQALQTFQTQTLGCI
VDRSLSRHYLSVFSSSTEGRKLCRHSLELLLEAYFCGRVGRNNHRFISQLMCDYVFKCMACESGVEVFEL
LDHCFAELNVGVTGLPVSDSRIIDGLVLPRDFSMYCPADGDIRMVIVTEILQPQFSSAGSEFVLNSETQF
QASQCWITDRTKTVMNHLRGQNVKLLLTSVKQPDLVIYCARLNSISVVECLSAEEVSLVQRITGLSPCVL
PEVASQCEISDSTLVKFCKPLILRSKRYVHLGLISTCAFIPHSMVLCGPVLGLVEQHERAFHGAFKMLRQ
LFTDLDLNYIIQTKQQCNPSPLAYDNSRERNHSPETDKYQDIVAKSKNKLETQTHLEVYSGLGASDTELR
AGKPWSAHKKTPIAPSQTDEMLKCLPPERSGIIDNCDLSIENHSTGNPTAEDTGTEISFEHLQVSDNAGK
GYTLPVMRKSLDTCTCQGYCSSTVPAGCVLPVGGSFEILMSYYLLSYAKQCRQSDETVISMLIADALLGI
PKILYKPKKGKDSFPHIYMRSLHALQASQPMVSGQSGFESVAGKYQLLTSVLQCLMKILTIDLIINIKRQ
PQKTADQESEDEF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027914
ORF Size 2139 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027914.1, NP_082190.1
RefSeq Size 2753 bp
RefSeq ORF 2142 bp
Locus ID 71769
UniProt ID Q9DBI2
Cytogenetics 10 D1
MW 78.9 kDa
Summary Probable molecular chaperone that assists the folding of proteins upon ATP hydrolysis. Plays a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation.UniProtKB/Swiss-Prot Function
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Documents
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"NM_027914" in other vectors (4)
SKU Description Size Price
MC221015 Bbs10 (untagged) - Mouse Bardet-Biedl syndrome 10 (human) (Bbs10), (10ug) 10 ug
$719.00
MG221925 Bbs10 (tGFP-tagged) - Mouse Bardet-Biedl syndrome 10 (human) (Bbs10), (10ug) 10 ug
$853.00
MR221925L3 Lenti ORF clone of Bbs10 (Myc-DDK-tagged) - Mouse Bardet-Biedl syndrome 10 (human) (Bbs10) 10 ug
$953.00
MR221925L4 Lenti ORF clone of Bbs10 (mGFP-tagged) - Mouse Bardet-Biedl syndrome 10 (human) (Bbs10) 10 ug
$953.00

Plasmid Map for MR221925

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR221925
Circular map for MR221925

file-alt Citations

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