Sergef (NM_013789) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR221697
Sergef (Myc-DDK-tagged) - Mouse secretion regulating guanine nucleotide exchange factor (Sergef)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sergef
Synonyms DelGEF; Gef; Gnef; Gnefr
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR221697 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCGGGAGTCCTGCGCCTCCGAGACTGTCTCTGCCGCTGCTGTGCTCTTCGCCTGGGGTGCAAACA
GCTATGGGCAACTTGGCCTTGGCCATAAGGAAGATGTGTTTCTGCCCCAGCAACTGAGTGACTTCTGTCA
AGCTGGGTGTATCAAGAGTGTCACAGGAGGAGGAGGGCATTCTGCAGTTGTCACAGATGGAGGAGATCTT
TTTGTTTGTGGCCTGAATAAAGATGGGCAGTTGGGGCTTGGTCACACAGAGGAAGTTCTGCGTTTTACCA
TCTGCAAGCCTCTCCGTGGTTGTCCCATCCGACAGGTGGCCTGTGGCTGGGACTTCACCATCATGCTTAC
AGAAAAAGGTCAAGTTCTGTCCTGTGGATCCAATGCCTTTGGCCAGTTAGGTGTGCCTCACGGCCCTCGC
AAATGCGTGGTCCCCCAGGCCATTGAGTGCCTGAGAGAGAAGGTTGTTTGTGTCGCTGCTGGACTGAGGC
ATGCCTTAGCCACTACAGCGACTGGCAGTGTGTTCCAGTGGGGGACTGGCTTGGCATCTTCTGGTCGACG
CCTGTGCCCTGGGCAGAATCTCCCACTGTTTTTGACAGCAAAGGAACCCAGCAGAGTGACAGGCCTGGAG
AATTCTACAGCAGTGTGTGCTGTTGCAGGATCTGACCACTCGGCCTCATTAACAGATACAGGAGAGCTGT
ACGTCTGGGGCCGTAACAAGCATGGGCAGCTGGCCTCCCGCGCTGTGTTCCTTCCTCTGCCCCAGAGAAT
AGAAGCACATTACTTTCAGGATGAAAAGGTCACTGCTGTCTGGAGTGGCTGGACACACCTGGTTGCTAAG
ACAGAAACTGGCAAGGTGTTTACCTGGGGTCGAGCAGACTATGGTCAGCTAGGGAGGAGGTTGGAGCCTC
CTGAAGCTCAGAAACCTGTAGAGCAAGACTCATCGCTCGCCTTCCAGGGGCCACAGAACAGTGTGCCTTC
ACCTCTGCATTGCCTGACAGGAGCAACAGAGATTTCTTGTGGCTCGGAGCACAACTTGGCAGTAATTAGG
GACAAGTGCTGCTCCTGGGGTTGGAACGAACACGGCATGTGTGGGGATGGCACTGAGTCCAATGTCTGGA
CTCCAACTCCAGTGCAGGCTCTGCCACCATCACCATCAAGACTCCTCCTCGTGGGCTGTGGGGCTGGCCA
CTCCTTGGCCGTATGTCAGCTGCCAGCACACCCTGTGCCATGCCAGGATCTCAAGGTCACCTGCCCTCTC
CCAGATGACACAGAGAACACTGAATCTCAGGGTGCCGTGGACAGAGACAGACTGGAAGGAGAGACGATCA
GTGACCTCAACCCAGACAGGACGAGAAATGGGGGTGGGGGGTGTGAGAGCGAGACCGTTCAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR221697 protein sequence
Red=Cloning site Green=Tags(s)

MARESCASETVSAAAVLFAWGANSYGQLGLGHKEDVFLPQQLSDFCQAGCIKSVTGGGGHSAVVTDGGDL
FVCGLNKDGQLGLGHTEEVLRFTICKPLRGCPIRQVACGWDFTIMLTEKGQVLSCGSNAFGQLGVPHGPR
KCVVPQAIECLREKVVCVAAGLRHALATTATGSVFQWGTGLASSGRRLCPGQNLPLFLTAKEPSRVTGLE
NSTAVCAVAGSDHSASLTDTGELYVWGRNKHGQLASRAVFLPLPQRIEAHYFQDEKVTAVWSGWTHLVAK
TETGKVFTWGRADYGQLGRRLEPPEAQKPVEQDSSLAFQGPQNSVPSPLHCLTGATEISCGSEHNLAVIR
DKCCSWGWNEHGMCGDGTESNVWTPTPVQALPPSPSRLLLVGCGAGHSLAVCQLPAHPVPCQDLKVTCPL
PDDTENTESQGAVDRDRLEGETISDLNPDRTRNGGGGCESETVQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013789
ORF Size 1392 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013789.2, NP_038817.1
RefSeq Size 1460 bp
RefSeq ORF 1395 bp
Locus ID 27414
UniProt ID Q80YD6
Cytogenetics 7 B3
MW 49.2 kDa
Summary Probable guanine nucleotide exchange factor (GEF), which may be involved in the secretion process.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013789" in other vectors (4)
SKU Description Size Price
MC216299 Sergef (untagged) - Mouse secretion regulating guanine nucleotide exchange factor (Sergef), (10ug) 10 ug
$503.00
MG221697 Sergef (tGFP-tagged) - Mouse secretion regulating guanine nucleotide exchange factor (Sergef), (10ug) 10 ug
$657.00
MR221697L3 Lenti ORF clone of Sergef (Myc-DDK-tagged) - Mouse secretion regulating guanine nucleotide exchange factor (Sergef) 10 ug
$757.00
MR221697L4 Lenti ORF clone of Sergef (mGFP-tagged) - Mouse secretion regulating guanine nucleotide exchange factor (Sergef) 10 ug
$757.00

Plasmid Map for MR221697

Download sequence for

Cloning scheme for MR221697

Circular map for MR221697

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