Tfdp1 (NM_009361) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR221670
Tfdp1 (Myc-DDK-tagged) - Mouse transcription factor Dp 1 (Tfdp1)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tfdp1
Synonyms Dp1; Drtf1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR221670 representing NM_009361
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAAAGATGCCAGTCTAATTGAAGCCAACGGAGAACTAAAGGTCTTTATAGACCAGAATCTTAGTC
CTGGGAAAGGTGTGGTATCTCTTGTAGCCGTCCACCCGTCCACAGTCAACACACTTGGGAAGCAGCTTTT
GCCAAAAACCTTCGGACAGTCCAATGTCAATATCACACAGCAAGTGGTGATTGGCACGCCTCAGAGACCG
GCAGCATCCAACACTATTGTGGTAGGAAGCCCACACACTCCCAACACGCATTTTGTGTCACAGAACCAGA
CGTCTGACTCCTCACCTTGGTCTGCTGGGAAGCGGAACAGGAAGGGCGAGAAGAATGGCAAGGGCCTGCG
GCATTTCTCCATGAAGGTGTGTGAGAAGGTGCAGAGGAAAGGAACCACCTCCTACAATGAGGTGGCTGAC
GAGCTGGTGGCAGAGTTCAGCGCTGCCGACAACCACATTCTACCAAACGAATCAGCTTATGACCAGAAGA
ACATCCGGCGGCGTGTCTACGATGCCTTAAATGTGCTAATGGCCATGAACATCATCTCCAAGGAGAAGAA
GGAGATCAAATGGATCGGCCTGCCCACCAACTCAGCTCAGGAGTGCCAGAACTTAGAGGTAGAGAGGCAG
AGGAGGCTGGAGAGGATCAAACAGAAGCAGTCACAGCTCCAGGAGCTCATCCTGCAGCAAATTGCCTTCA
AGAACTTGGTGCAGAGAAATCGCCAAGCTGAGCAGCAGGCCCGCAGGCCGCCTCCTCCCAACTCTGTCAT
CCACTTGCCCTTCATCATTGTCAACACCAGCAGGAAGACAGTCATTGACTGCAGCATCTCCAATGACAAA
TTTGAGTATCTGTTTAACTTTGACAACACGTTTGAGATCCACGATGACATTGAGGTGCTCAAGCGCATGG
GCATGGCATGTGGGCTGGAGTCTGGCAACTGCTCTGCTGAAGACCTCAAGGTGGCCAGAAGTTTGGTACC
AAAAGCTCTAGAACCATACGTGACAGAAATGGCTCAGGGATCCATTGGTGGAGTATTCGTCACGACAACA
GGTTCTACATCCAATGGCACAAGGCTTTCTGCCAGTGATTTGAGCAATGGTGCAGATGGGATGCTGGCCA
CGAGCTCCAATGGGTCTCAGTACAGCGGCTCCAGGGTCGAGACCCCTGTGTCCTACGTTGGGGAGGATGA
TGACGACGATGATGACTTTAATGAGAACGACGAGGAGGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR221670 representing NM_009361
Red=Cloning site Green=Tags(s)

MAKDASLIEANGELKVFIDQNLSPGKGVVSLVAVHPSTVNTLGKQLLPKTFGQSNVNITQQVVIGTPQRP
AASNTIVVGSPHTPNTHFVSQNQTSDSSPWSAGKRNRKGEKNGKGLRHFSMKVCEKVQRKGTTSYNEVAD
ELVAEFSAADNHILPNESAYDQKNIRRRVYDALNVLMAMNIISKEKKEIKWIGLPTNSAQECQNLEVERQ
RRLERIKQKQSQLQELILQQIAFKNLVQRNRQAEQQARRPPPPNSVIHLPFIIVNTSRKTVIDCSISNDK
FEYLFNFDNTFEIHDDIEVLKRMGMACGLESGNCSAEDLKVARSLVPKALEPYVTEMAQGSIGGVFVTTT
GSTSNGTRLSASDLSNGADGMLATSSNGSQYSGSRVETPVSYVGEDDDDDDDFNENDEED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009361
ORF Size 1230 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009361.3, NP_033387.1
RefSeq Size 1700 bp
RefSeq ORF 1233 bp
Locus ID 21781
UniProt ID Q08639
Cytogenetics 8 A1.1
MW 45.7 kDa
Summary Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTCCGCGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The E2F1:DP complex appears to mediate both cell proliferation and apoptosis. Blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters (PubMed:20176812).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009361" in other vectors (4)
SKU Description Size Price
MC209518 Tfdp1 (untagged) - Mouse transcription factor Dp 1 (Tfdp1), (10ug) 10 ug
$503.00
MG221670 Tfdp1 (tGFP-tagged) - Mouse transcription factor Dp 1 (Tfdp1), (10ug) 10 ug
$657.00
MR221670L3 Lenti ORF clone of Tfdp1 (Myc-DDK-tagged) - Mouse transcription factor Dp 1 (Tfdp1) 10 ug
$757.00
MR221670L4 Lenti ORF clone of Tfdp1 (mGFP-tagged) - Mouse transcription factor Dp 1 (Tfdp1) 10 ug
$757.00

Plasmid Map for MR221670

Download sequence for

Cloning scheme for MR221670

Circular map for MR221670

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