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Gphb5 (NM_175644) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR221569
Gphb5 (Myc-DDK-tagged) - Mouse glycoprotein hormone beta 5 (Gphb5)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gphb5
Synonyms OGH; Zlut1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR221569 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGTTGGTATACCTTGTCCTTGGTGCAGTGGCCCTCCTTCTCCTGGGTGGCCCTGACTCTGTCCTCA
GCAGCTCCAGTGGGAACCTGCACACTTTTGTGGGCTGTGCTGTGAGGGAATTCACTTTCATGGCCAAGAA
GCCAGGCTGCAGGGGACTTCGGATCACCACAGATGCCTGCTGGGGCCGCTGCGAGACCTGGGAGAAACCC
ATCCTGGAGCCTCCCTACATTGAAGCCTATCATCGAGTGTGTACATACAATGAGACCAGACAGGTGACAG
TGAAGCTGCCTAACTGTGCCCCTGGAGTCGATCCTTTCTACACCTACCCTATGGCTGTCCGATGTGACTG
TGGGGCGTGTTCCACTGCCACCACTGAGTGTGAGACCATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR221569 protein sequence
Red=Cloning site Green=Tags(s)

MKLVYLVLGAVALLLLGGPDSVLSSSSGNLHTFVGCAVREFTFMAKKPGCRGLRITTDACWGRCETWEKP
ILEPPYIEAYHRVCTYNETRQVTVKLPNCAPGVDPFYTYPMAVRCDCGACSTATTECETI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175644
ORF Size 390 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175644.3, NP_783575.2
RefSeq Size 1614 bp
RefSeq ORF 393 bp
Locus ID 217674
UniProt ID Q812B2
Cytogenetics 12 C3
MW 14.2 kDa
Summary Functions as a heterodimeric glycoprotein hormone with GPHA2 able to bind and activate the thyroid-stimulating hormone receptor (TSHR), leading to increased cAMP production. Plays a central role in controlling thyroid cell metabolism.UniProtKB/Swiss-Prot Function
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"NM_175644" in other vectors (4)
SKU Description Size Price
MC212670 Gphb5 (untagged) - Mouse glycoprotein hormone beta 5 (Gphb5), (10ug) 10 ug
$165.00
MG221569 Gphb5 (tGFP-tagged) - Mouse glycoprotein hormone beta 5 (Gphb5), (10ug) 10 ug
$350.00
MR221569L3 Lenti ORF clone of Gphb5 (Myc-DDK-tagged) - Mouse glycoprotein hormone beta 5 (Gphb5) 10 ug
$450.00
MR221569L4 Lenti ORF clone of Gphb5 (mGFP-tagged) - Mouse glycoprotein hormone beta 5 (Gphb5) 10 ug
$450.00

Plasmid Map for MR221569

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR221569
Circular map for MR221569

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