Vgll2 (NM_153786) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR221554
Vgll2 (Myc-DDK-tagged) - Mouse vestigial like 2 homolog (Drosophila) (Vgll2)
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vgll2
Synonyms C130057C21Rik; Vgl; vgl-2; Vi; VIT; VITO-1; Vito1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR221554 representing NM_153786
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTGTCTGGATGTTATGTACCAGGTCTACGGTCCCCCGCAGCCTTATTTCGCAGCCGCCTACACTC
CCTACCACCAGAAACTAGCCTACTACTCAAAAATGCAGGAAGCTCAAGAGTGCGCCAGCCCTGGCAGCAG
TGCCAGCGGGAGCTCCTCATTTTCCAACCCAACCCCAGCCAGTGTCAAGGAAGAGGAGGGCAGCCCAGAG
AAAGAGCGCCCGCCCGAAGCTGAGTACATCAACTCCAGATGTGTCCTCTTCACCTACTTCCAGGGGGACA
TCAGCTCTGTGGTGGACGAACATTTCAGTAGGGCCCTTAGCCACCCAAGCAGCTACACCCCAAGCTGTAC
CAGCAGCAAAGCACACAGAAGCTCTGGACCCTGGAGAGCAGAAGGCACCTTCCCGATGAGCCAGCGCAGC
TTCCCCGCCTCCTTCTGGAACAGCGCGTACCAGGCGCCTGTGCCCGCGCCACTAGGCAGTCCTCTGGCCG
CCGCACACTCGGAGCTGCCCTTTGCCACCGACCCCTACTCTCCCGCCACTCTGCACGGCCACCTGCACCA
GGGCGCGGCCGACTGGCACCACGCGCACCCGCACCACGCGCACCCGCACCATCCCTATGCGCTGGGCGGC
GCCCTGGGAGCACAAGCCTCTGCCTACCCGCGGCCAGCAGTGCACGAGGTCTACGCGCCCCACTTCGACC
CGCGCTATGGGCCGCTGCTCATGCCCGCGGCCACTGGCCGCCCCGGCCGCCTGGCCCCTGCCTCGGCGCC
GGCTCCCGGCAGCCCTCCCTGCGAGCTTGCGGCCAAGGGCGAGCCGGCGGGCAGCGCATGGGCTGCGCCC
GGGGGACCCTTCGTGAGCCCCACGGGGGATGTGGCCCAGAGCCTGGGTCTCAGCGTGGACTCAGGTAAGC
GGAGGAGGGAATGCAGTCTCCCCTCTGCCCCTCCGGCACTGTACCCGACTCTGGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR221554 representing NM_153786
Red=Cloning site Green=Tags(s)

MSCLDVMYQVYGPPQPYFAAAYTPYHQKLAYYSKMQEAQECASPGSSASGSSSFSNPTPASVKEEEGSPE
KERPPEAEYINSRCVLFTYFQGDISSVVDEHFSRALSHPSSYTPSCTSSKAHRSSGPWRAEGTFPMSQRS
FPASFWNSAYQAPVPAPLGSPLAAAHSELPFATDPYSPATLHGHLHQGAADWHHAHPHHAHPHHPYALGG
ALGAQASAYPRPAVHEVYAPHFDPRYGPLLMPAATGRPGRLAPASAPAPGSPPCELAAKGEPAGSAWAAP
GGPFVSPTGDVAQSLGLSVDSGKRRRECSLPSAPPALYPTLG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153786
ORF Size 966 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153786.2
RefSeq Size 1483 bp
RefSeq ORF 969 bp
Locus ID 215031
UniProt ID Q8BGW8
Cytogenetics 10 B3
MW 34.5 kDa
Summary This gene is a member of the Vestigial-like (Vgl) gene family and is upregulated during muscle differentiation. The product of this gene interacts with and modifies the DNA-binding properties of the transcription factor, TEF-1, and is important for muscle tissue development. Reduced expression of this gene leads to a reduction in the terminal differentiation of muscle cells. Alternate splicing results in multiple protein isoforms. provided by RefSeq, Jul 2014
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_153786" in other vectors (4)
SKU Description Size Price
MC212617 Vgll2 (untagged) - Mouse vestigial like 2 homolog (Drosophila) (Vgll2), (10ug) 10 ug
$450.00
MG221554 Vgll2 (tGFP-tagged) - Mouse vestigial like 2 homolog (Drosophila) (Vgll2), (10ug) 10 ug
$650.00
MR221554L3 Lenti ORF clone of Vgll2 (Myc-DDK-tagged) - Mouse vestigial like 2 homolog (Drosophila) (Vgll2) 10 ug
$750.00
MR221554L4 Lenti ORF clone of Vgll2 (mGFP-tagged) - Mouse vestigial like 2 homolog (Drosophila) (Vgll2) 10 ug
$750.00

Plasmid Map for MR221554

Download sequence for

Cloning scheme for MR221554

Circular map for MR221554

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products