Vgll2 (NM_153786) Mouse Untagged Clone

SKU
MC212617
Vgll2 (untagged) - Mouse vestigial like 2 homolog (Drosophila) (Vgll2), (10ug)
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Vgll2
Synonyms C130057C21Rik; Vgl; vgl-2; Vi; VIT; VITO-1; Vito1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC212617 representing NM_153786
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTGTCTGGATGTTATGTACCAGGTCTACGGTCCCCCGCAGCCTTATTTCGCAGCCGCCTACACTC
CCTACCACCAGAAACTAGCCTACTACTCAAAAATGCAGGAAGCTCAAGAGTGCGCCAGCCCTGGCAGCAG
TGCCAGCGGGAGCTCCTCATTTTCCAACCCAACCCCAGCCAGTGTCAAGGAAGAGGAGGGCAGCCCAGAG
AAAGAGCGCCCGCCCGAAGCTGAGTACATCAACTCCAGATGTGTCCTCTTCACCTACTTCCAGGGGGACA
TCAGCTCTGTGGTGGACGAACATTTCAGTAGGGCCCTTAGCCACCCAAGCAGCTACACCCCAAGCTGTAC
CAGCAGCAAAGCACACAGAAGCTCTGGACCCTGGAGAGCAGAAGGCACCTTCCCGATGAGCCAGCGCAGC
TTCCCCGCCTCCTTCTGGAACAGCGCGTACCAGGCGCCTGTGCCCGCGCCACTAGGCAGTCCTCTGGCCG
CCGCACACTCGGAGCTGCCCTTTGCCACCGACCCCTACTCTCCCGCCACTCTGCACGGCCACCTGCACCA
GGGCGCGGCCGACTGGCACCACGCGCACCCGCACCACGCGCACCCGCACCATCCCTATGCGCTGGGCGGC
GCCCTGGGAGCACAAGCCTCTGCCTACCCGCGGCCAGCAGTGCACGAGGTCTACGCGCCCCACTTCGACC
CGCGCTATGGGCCGCTGCTCATGCCCGCGGCCACTGGCCGCCCCGGCCGCCTGGCCCCTGCCTCGGCGCC
GGCTCCCGGCAGCCCTCCCTGCGAGCTTGCGGCCAAGGGCGAGCCGGCGGGCAGCGCATGGGCTGCGCCC
GGGGGACCCTTCGTGAGCCCCACGGGGGATGTGGCCCAGAGCCTGGGTCTCAGCGTGGACTCAGGTAAGC
GGAGGAGGGAATGCAGTCTCCCCTCTGCCCCTCCGGCACTGTACCCGACTCTGGGCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_153786
Insert Size 969 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153786.1, NP_722481.1
RefSeq Size 1667 bp
RefSeq ORF 969 bp
Locus ID 215031
UniProt ID Q8BGW8
Cytogenetics 10 B3
Summary This gene is a member of the Vestigial-like (Vgl) gene family and is upregulated during muscle differentiation. The product of this gene interacts with and modifies the DNA-binding properties of the transcription factor, TEF-1, and is important for muscle tissue development. Reduced expression of this gene leads to a reduction in the terminal differentiation of muscle cells. Alternate splicing results in multiple protein isoforms. provided by RefSeq, Jul 2014
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_153786" in other vectors (4)
SKU Description Size Price
MG221554 Vgll2 (tGFP-tagged) - Mouse vestigial like 2 homolog (Drosophila) (Vgll2), (10ug) 10 ug
$650.00
MR221554 Vgll2 (Myc-DDK-tagged) - Mouse vestigial like 2 homolog (Drosophila) (Vgll2) 10 ug
$450.00
MR221554L3 Lenti ORF clone of Vgll2 (Myc-DDK-tagged) - Mouse vestigial like 2 homolog (Drosophila) (Vgll2) 10 ug
$750.00
MR221554L4 Lenti ORF clone of Vgll2 (mGFP-tagged) - Mouse vestigial like 2 homolog (Drosophila) (Vgll2) 10 ug
$750.00

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