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Adamts8 (NM_013906) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR221476
Adamts8 (Myc-DDK-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 8 (Adamts8)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $829.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

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PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
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Other products for "Adamts8 or NM_013906"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Adamts8
Synonyms METH-2; METH2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR221476 representing NM_013906
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCCGCGACCCCACCACCACCGGGTGGCCGCCCCTCCTGCTGCTGCTATTGCAGCTGCCGCCGCCGC
CACTCGTCTGCGGCGCCCCGGCGGGGCCGGGAACCGGGGCGCAGGCCTCGGAGCTAGTGGTGCCCACGCG
GTTGCCCGGCAGCGCGAGCGAGCTCGCCTTCCACCTGTCCGCCTTCGGCCAGGGCTTCGTGCTGCGCCTG
GCGCCTGACGCCAGCTTCCTGGCGCCGGAATTCAAGATCGAGCGCCTCGGGGGCTCGAGCGCGGCGGCCG
GGGGCGAGCCGGGACTGCGTGGCTGCTTCTTCTCTGGCACAGTGAATGGAGAACGGGAGTCGCTGGCGGC
GATGAGCCTGTGTCGCGGGCTGAGCGGCTCGTTCTTGCTGGCAGGCGAGGAGTTCACCATCCAGCCACAG
GGCGCTGGGGACTCCCTGGACCAGCCTCATCGCCTGCAGCGCTGGGGGCCGGGACAGCGCCGCGAAGACC
CCGGGCTCGCTGCCGCCGAAGTTTTCCCCCTCCCTCAAGGACTGGAGTGGGAGGTGGAGATGGGTAATGG
GCAGGGACAGGAGAGAAGTGACAACGAAGAGGACAGGAAGCAGGACAAGGAGGGGTTGCTCAAAGAGACA
GAAGACTCCCGCAAAGTGCCACCACCCTTCGGATCCAAAACTAGAAGCAAGAGGTTTGTGTCCGAGGCTC
GCTTCGTGGAAACACTTCTGGTGGCTGATGCGTCCATGGCTGCCTTCTATGGGACCGACCTGCAGAACCA
CATCCTCACGGTGATGTCAATGGCAGCCCGAATCTACAAGCACCCGAGCATCAGGAACTCCGTCAACCTT
GTGGTGGTGAAAGTGCTAATAGTGGAAGAGGAAGGATGGGGCCCGGAGGTGTCGGACAACGGGGGGCTCA
CACTGCGCAACTTCTGCAGCTGGCAACGGCGTTTCAACAAGCCCAGTGACCGCCACCCGGAGCACTATGA
CACTGCCATCTTGTTCACCAGACAGAACTTCTGTGGGAAGGGAGAGCAGTGTGACACCCTGGGGATGGCA
GACGTTGGCACCATCTGTGACCCCGACAAGAGCTGCTCAGTGATCAAGGATGAGGGACTGCAGGCAGCCT
ACACCCTGGCCCATGAGCTAGGGCACGTTCTCAGCATGCCCCATGATGATTCTAAGCCCTGTGTGAGATT
GTTTGGGCCCATGGGCAAGTACCACATGATGGCGCCATTCTTCATCCACGTGAACAAGACGCTGCCCTGG
TCTCCCTGCAGTGCTGTCTACCTCACAGAGCTCCTGGATGATGGTCACGGAGATTGTCTTCTGGATGCCC
CCACCTCGGTTCTGCCCCTCCCCACAGGCCTCCCGGGCCACAGCACCCTCTACGAGCTGGACCAGCAGTG
CAAGCAGATCTTTGGGCCTGATTTCCGACACTGCCCCAACACCTCTGTGGAGGACATCTGTGTCCAGCTC
TGGTGCCGTCATCGGGATAGTGATGAGCCCATTTGCCACACAAAGAATGGTAGCCTGCTCTGGGCTGATG
GTACACCCTGTGGCCCTGGGCACCTGTGCCTGGATGGTAGCTGTGTACTCAAGGAGGATGTGGAGAATCC
CAAGGCTGTGGTAGATGGAGACTGGGGTCCCTGGAGACCCTGGGGACAATGTTCTCGCACCTGTGGTGGA
GGGATACAATTCTCGAACCGTGAATGTGATAATCCAATGCCTCAGAATGGAGGAAGATTTTGCCTGGGTG
AAAGAGTCAAGTACCAATCATGCAACACAGAGGAATGTCCACCAAACGGAAAAAGCTTCCGGGAGCAGCA
GTGTGAGAAATATAATGCCTACAACCACACTGACCTGGATGGGAATTTCCTGCAGTGGGTCCCCAAGTAT
TCAGGAGTGTCCCCCCGAGACCGATGCAAGCTGTTTTGCAGAGCCCGTGGGAGGAGTGAGTTCAAAGTGT
TTGAAGCTAAGGTGATCGATGGCACTCTGTGTGGACCGGATACTCTGTCCATCTGCGTCCGGGGGCAATG
TGTTAAGGCTGGCTGTGACCATGTGGTGAACTCACCTAAGAAGCTGGACAAATGTGGGGTGTGTGGGGGC
AAAGGCACTGCCTGTAGGAAGATCTCCGGTTCTTTCACCCCCTTCAGTTATGGCTACAATGACATTGTCA
CCATCCCAGCTGGTGCCACAAACATTGATGTGAAACAGCGGAGTCACCCAGGGGTCAGGAACGACGGCAG
CTACCTGGCGCTGAAGACAGCCAATGGGCAGTACCTGCTCAATGGTAACCTGGCCATCTCTGCCATAGAG
CAAGACATCTTGGTGAAGGGGACCATCCTGAAGTACAGTGGCTCCATGGCTACCCTGGAGCGGCTGCAGA
GCTTCCAGGCCCTGCCTGAGCCTCTTACAGTACAGCTCCTGACTGTGTCTGGTGAGGTCTTCCCTCCAAA
AGTCAGATATACCTTCTTTGTCCCCAATGACATGGACTTCAGCGTGCAGAATAGCAAGGAAAGAGCAACC
ACCAACATCATTCAGTCACTGCCCTCTGCGGAGTGGGTTCTGGGAGACTGGTCTGAATGTCCGAGCACGT
GCAGAGGTAGCTGGCAGCGGCGGACTGTGGAATGCAGGGACCCCTCAGGTCAGGCCTCTGACACCTGTGA
TGAGGCTCTGAAACCTGAGGATGCCAAGCCCTGTGGAAGCCAGCCGTGTCCCCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR221476 representing NM_013906
Red=Cloning site Green=Tags(s)

MLRDPTTTGWPPLLLLLLQLPPPPLVCGAPAGPGTGAQASELVVPTRLPGSASELAFHLSAFGQGFVLRL
APDASFLAPEFKIERLGGSSAAAGGEPGLRGCFFSGTVNGERESLAAMSLCRGLSGSFLLAGEEFTIQPQ
GAGDSLDQPHRLQRWGPGQRREDPGLAAAEVFPLPQGLEWEVEMGNGQGQERSDNEEDRKQDKEGLLKET
EDSRKVPPPFGSKTRSKRFVSEARFVETLLVADASMAAFYGTDLQNHILTVMSMAARIYKHPSIRNSVNL
VVVKVLIVEEEGWGPEVSDNGGLTLRNFCSWQRRFNKPSDRHPEHYDTAILFTRQNFCGKGEQCDTLGMA
DVGTICDPDKSCSVIKDEGLQAAYTLAHELGHVLSMPHDDSKPCVRLFGPMGKYHMMAPFFIHVNKTLPW
SPCSAVYLTELLDDGHGDCLLDAPTSVLPLPTGLPGHSTLYELDQQCKQIFGPDFRHCPNTSVEDICVQL
WCRHRDSDEPICHTKNGSLLWADGTPCGPGHLCLDGSCVLKEDVENPKAVVDGDWGPWRPWGQCSRTCGG
GIQFSNRECDNPMPQNGGRFCLGERVKYQSCNTEECPPNGKSFREQQCEKYNAYNHTDLDGNFLQWVPKY
SGVSPRDRCKLFCRARGRSEFKVFEAKVIDGTLCGPDTLSICVRGQCVKAGCDHVVNSPKKLDKCGVCGG
KGTACRKISGSFTPFSYGYNDIVTIPAGATNIDVKQRSHPGVRNDGSYLALKTANGQYLLNGNLAISAIE
QDILVKGTILKYSGSMATLERLQSFQALPEPLTVQLLTVSGEVFPPKVRYTFFVPNDMDFSVQNSKERAT
TNIIQSLPSAEWVLGDWSECPSTCRGSWQRRTVECRDPSGQASDTCDEALKPEDAKPCGSQPCPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013906
ORF Size 2715 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013906.3, NP_038934.2
RefSeq Size 3632 bp
RefSeq ORF 2718 bp
Locus ID 30806
Cytogenetics 9 16.49 cM
MW 99.4 kDa
Summary This gene encodes a member of "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. This gene is expressed in mouse lung, heart and macrophage-rich areas of atherosclerotic plaques. The encoded preproprotein undergoes proteolytic processing to generate an active, zinc-dependent aggrecanase enzyme. This gene is located adjacent to a related ADAMTS gene on chromosome 9. provided by RefSeq, May 2016
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"NM_013906" in other vectors (4)
SKU Description Size Price
MC222526 Adamts8 (untagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 8 (Adamts8), (10ug) 10 ug
$912.00
MG221476 Adamts8 (tGFP-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif 8 (Adamts8), (10ug) 10 ug
$1,029.00
MR221476L3 Lenti ORF clone of Adamts8 (Myc-DDK-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 8 (Adamts8) 10 ug
$1,129.00
MR221476L4 Lenti ORF clone of Adamts8 (mGFP-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 8 (Adamts8) 10 ug
$1,129.00

Plasmid Map for MR221476

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR221476
Circular map for MR221476

file-alt Citations

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