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Cylc1 (NM_026134) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR221284
Cylc1 (Myc-DDK-tagged) - Mouse cylicin, basic protein of sperm head cytoskeleton 1 (Cylc1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $596.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cylc1
Synonyms 4930401M13Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR221284 representing NM_026134
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCTTTCAAAACTGGACAGTGAAAAATTGACTATTGAAGATGTTCAAACTTCAAGTTCTTCCTGTC
GACGAGAAATAAATACCACAACATATGATGATTACATTCTGAGCATTCAAACTAGCGAAAAACAGAATCA
AGAACATTTTGTTTTGACATTTCCCAAAACACCTATGCCAGATAAAAAGAAGAGATCAGGACCTTCAGAA
TTAGAAGTTGCAGTTCCTATACAAGTGAAAAGGAAAATTGAGAAAGATCAGAAACCAACTCACGTATGGA
TAAACCAGTTTTTAAGAGACATTTTCTTAAAGTCATCCTTTTCTCGGCCGTTTATTACACAAGCTCCATT
CAAATATCTTTATAATCCTCAAAACCATTATACAATGGCAGAAAGTAGAAAGTCCAAAAATGATGAAAGA
AGAAAAACTTTGAAGATAAAGTTCAGGGGAAAAATAAGCTCATGTGTAGTAAATCTGGAGCCCATGAGAA
CAATAACCAATGGGGAACCTGAAATACTAGGCAATACAGAAAAAAATCCATCAAAATCATCACATAAAAT
TAAATTGCCTAAGACGTCAAATTCTACATCAGAAACAAATCTAGAATACAACAATTCAAAGAAAACCTTG
GAAATGAGTTTGAGAAATGGCAACAAAAATTCAATGAACTTTGTTCTGAAGGGGAATGCAGCCACATGTT
GCAAGGACAATCCCAATACAGACTCTAAGAAGAGTGTTGAAGAATTTTCTGATGACATATCAGAGTGTAT
AAACAGCTCAAATATGGATTTAATGTTGCGTCTAAATGAGTTCAGAGCTGAATTCACAGATTTGGATGTA
TGGTCAACAAATTGCTCACAGAACAATGCAAAGAAGCCTTTAAAGACGGGAGGAAAGAAGGAAAGGGACT
CAGATATTGATTCTGGAGGCTCAAAAGATGCAAAGAAAGAGGGAAAGAAAAAAGGCAAGAGAGAATCCAG
GAAAAAGAGGAATACAGAATCGAGTGATGCTGAATCTGGTGACTCCAAGGATGGAAAGAAAAAATCAAAA
CACGATAAAAAAAATGAAATTAAGAAAAAGAAGGATACAGACTCTACTGGTAGTGGATCTGGGGCGTCCA
TGGTTTCAAAGAAAGGTAAGACGGAAAAGAAGAGTACAGGAAAAAAGAGTACAGGATCTACTGGTAGTGA
ATCTGTAGATTCAAAGAGTACAAATAAAGTAAAGAAACAAGTAAAAAAAGGTGTCATGAAGAAAGCAGTC
TCTACTGATTCTGAATCAGATGCATCATCAAAGAAGAGCAAGAAAGATGAAAAGAAGGAAAATAAAGGGA
GAAAGAAGAAGCCTATTAAGGATACAGAGTCTACTGATGCTGATTCTGAATCTGAAGGTGACTCAACAGG
TAAAAAGAATGAAAAGAAGGATAAGAAAATTACAAAGAAAGGAGAAAAGAAGGATGCAAAGAAGAATACA
GCATCCTCTGAAAGTGAGTCTGATTTGGGAGTAAACAAAAAGAAAACTAAAATAAAGGAAATAGTGAGTT
TCAGTGATTCAACATCTGATTCATATTCTAAGGCAGGCAGAAGAAAAAATGTAAGGCGCTCAGATTCCGA
ATCTGAAGATTCGTCAGGGTTCAGAGTTTTAAAGTCAACTGATGATTCAGAGGCCTCATCCACAGACTCC
AAGACGGGAATGCCAGGAATGAGAAGAGGATTCAGATCATTATCCAAAAAGACTACTTTCAATGAAAGGG
GGAAAAGAAGTGTGACCGGTAGAATCCCTTCATCAAGAGAAAGACTACCTTTCCCTCCTTGTGAGCCTTT
TAGAGCATCACCTAAGCCTGTGCATGTCTGTAAGTGCAAGGAATCTCCATCTCCAAAAGCAAGATATGCT
CCTTTGGTATGTTTACTAATAATTAATACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR221284 representing NM_026134
Red=Cloning site Green=Tags(s)

MSLSKLDSEKLTIEDVQTSSSSCRREINTTTYDDYILSIQTSEKQNQEHFVLTFPKTPMPDKKKRSGPSE
LEVAVPIQVKRKIEKDQKPTHVWINQFLRDIFLKSSFSRPFITQAPFKYLYNPQNHYTMAESRKSKNDER
RKTLKIKFRGKISSCVVNLEPMRTITNGEPEILGNTEKNPSKSSHKIKLPKTSNSTSETNLEYNNSKKTL
EMSLRNGNKNSMNFVLKGNAATCCKDNPNTDSKKSVEEFSDDISECINSSNMDLMLRLNEFRAEFTDLDV
WSTNCSQNNAKKPLKTGGKKERDSDIDSGGSKDAKKEGKKKGKRESRKKRNTESSDAESGDSKDGKKKSK
HDKKNEIKKKKDTDSTGSGSGASMVSKKGKTEKKSTGKKSTGSTGSESVDSKSTNKVKKQVKKGVMKKAV
STDSESDASSKKSKKDEKKENKGRKKKPIKDTESTDADSESEGDSTGKKNEKKDKKITKKGEKKDAKKNT
ASSESESDLGVNKKKTKIKEIVSFSDSTSDSYSKAGRRKNVRRSDSESEDSSGFRVLKSTDDSEASSTDS
KTGMPGMRRGFRSLSKKTTFNERGKRSVTGRIPSSRERLPFPPCEPFRASPKPVHVCKCKESPSPKARYA
PLVCLLIINT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026134
ORF Size 1920 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026134.2, NP_080410.2
RefSeq Size 1963 bp
RefSeq ORF 1923 bp
Locus ID 67407
UniProt ID G3X9R0
Cytogenetics X E1
MW 71.5 kDa
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Documents
Resources
"NM_026134" in other vectors (4)
SKU Description Size Price
MC220001 Cylc1 (untagged) - Mouse cylicin, basic protein of sperm head cytoskeleton 1 (Cylc1), (10ug) 10 ug
$656.00
MG221284 Cylc1 (tGFP-tagged) - Mouse cylicin basic protein of sperm head cytoskeleton 1 (Cylc1), (10ug) 10 ug
$796.00
MR221284L3 Lenti ORF clone of Cylc1 (Myc-DDK-tagged) - Mouse cylicin, basic protein of sperm head cytoskeleton 1 (Cylc1) 10 ug
$896.00
MR221284L4 Lenti ORF clone of Cylc1 (mGFP-tagged) - Mouse cylicin, basic protein of sperm head cytoskeleton 1 (Cylc1) 10 ug
$896.00

Plasmid Map for MR221284

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR221284
Circular map for MR221284

file-alt Citations

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