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Mmp27 (NM_001030289) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR221237
Mmp27 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 27 (Mmp27)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $513.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mmp27
Synonyms Gm180
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR221237 representing NM_001030289
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCATACAAACAGAAACAAGCTTTAACTGAAGAGACAGGAATGAAGTGCCTTCTGTCTCTGATGGTTA
ATTTTATAACACTTTCCGCTGCATTTCCTCCAGACAGGAAGGACAAAAATGAGGAGAACAACCAACTGGC
CCAGGCATATCTCAACCAGTTCTACTCTCTTGAAATAGAAGGGAGTCATTTTGTCCAAAGCAAGAACAGG
AGTCTCTTTGATGGAAAACTTCGGGAAATGCAGGCATTTTTCGGATTGACAGTGACTGGAAAACTGGATT
CAGACACACTTGCGATCATGAAAGTGCCCAGGTGTGGGGTACCAGATGTGGGGCAATATGGCTACACACT
CCCTGGGTGGAGAAAATACAGCCTTACATACAGAATAATGAACTATACTCCTGATATGACACCAGCTGAT
GTGGATGAGGCTATTCAGAAAGCTCTACAAGTTTGGAGCAAGGTCACTCCACTGACGTTTACCAGGATAT
CCAAGGGGGTTGCAGATATAATGATAGCATTCAGGACAGGAGGTAAGGCTTGCAGTGAGGGGAGTGACTT
CTTCGAGGATCACAGGGAGCTGACCTATCTCCCCATCAACTGTCTTTCAGTCCATGGCTGGTGTCCTCGT
CACTTTGATGGTCCTCTGGGAGTCCTTGGCCATGCCTTTCCTCCTGGTCTGGGTCTAGGTGGTGACACTC
ACTTTGACGAAGATGAAACATGGATAGCCAAGGATGGGGAAGGGTTCAACTTGTTTCTTGTGGCTGCTCA
TGAATTTGGTCACTCTCTGGGGCTGTCCCACTCCAATGATCAAACAGCCTTGATGTTCCCCAATTACATC
TCCCTGGATCCTAGCAAATACCCACTTTCTCAGGATGATATTGATGGGATCCAGTCCATCTATGGAAGTC
CACCTAAGGTAACCACCAAGCCAAGTGGAAATTCTGAACCCCACGCCTGTGACCCCACCTTGACTTTTGA
TGCTATCACTACTTTCCGCAGGGAAGTTATGTTCTTTAAAGGCAGGCACTTATGGAGGGTCTACTCTGAT
ATTGCTGGTGCTGAGTTTGAGTTTATTGATTCCTTCTGGCCATCTCTGCCAGCTGATCTTCAAGCTGCCT
ATGAAAGCCCCAGAGATGAGCTCCTTGTTTTTAAAGATGAGAATTTCTGGGTCATCAGGGGATATTCTGT
CTTGCCCGGTTACCCCAAATCCATCCACACACTCGGATTTCCAAGACGTGTGAAGAAAATTGATGCAGCC
GTCTGTGATCATGATACAAGAAAAACCTTCTTTTTTGTTGGCATCTGGTGCTGGAGGTATGATGAGATGG
CACAAGCAATGGACAGAGGATTCCCACAGAGGATAATAAAGTGCTTCCCAGGAATTCGCCTCCGTGTGGA
TGCTGTCTTCCAACATAATGGATTCCTCTATTTCTTCCATGGGTCGAGGCAATTTGAATATGACATGAAG
GCGAAAAATATCACCCAAGTGATCAAAACCAATTCTTGGTTCCTGTGTAACGAACCATTAAACGCATCAT
TCAATGTCAGTGTCAAAGGAAAAGCAAATTCAATTGGCACAGTGATATTACATCATAAAAGGTTAAGCTT
GCTCACTTTCAGTATTGTTCATGTGCTGACAAAAACATACAAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR221237 representing NM_001030289
Red=Cloning site Green=Tags(s)

MPYKQKQALTEETGMKCLLSLMVNFITLSAAFPPDRKDKNEENNQLAQAYLNQFYSLEIEGSHFVQSKNR
SLFDGKLREMQAFFGLTVTGKLDSDTLAIMKVPRCGVPDVGQYGYTLPGWRKYSLTYRIMNYTPDMTPAD
VDEAIQKALQVWSKVTPLTFTRISKGVADIMIAFRTGGKACSEGSDFFEDHRELTYLPINCLSVHGWCPR
HFDGPLGVLGHAFPPGLGLGGDTHFDEDETWIAKDGEGFNLFLVAAHEFGHSLGLSHSNDQTALMFPNYI
SLDPSKYPLSQDDIDGIQSIYGSPPKVTTKPSGNSEPHACDPTLTFDAITTFRREVMFFKGRHLWRVYSD
IAGAEFEFIDSFWPSLPADLQAAYESPRDELLVFKDENFWVIRGYSVLPGYPKSIHTLGFPRRVKKIDAA
VCDHDTRKTFFFVGIWCWRYDEMAQAMDRGFPQRIIKCFPGIRLRVDAVFQHNGFLYFFHGSRQFEYDMK
AKNITQVIKTNSWFLCNEPLNASFNVSVKGKANSIGTVILHHKRLSLLTFSIVHVLTKTYN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001030289
ORF Size 1653 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001030289.1, NP_001025460.1
RefSeq Size 1656 bp
RefSeq ORF 1656 bp
Locus ID 234911
Cytogenetics 9 A1
MW 63 kDa
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Documents
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"NM_001030289" in other vectors (4)
SKU Description Size Price
MC218889 Mmp27 (untagged) - Mouse matrix metallopeptidase 27 (Mmp27), (10ug) 10 ug
$565.00
MG221237 Mmp27 (tGFP-tagged) - Mouse matrix metallopeptidase 27 (Mmp27), (10ug) 10 ug
$713.00
MR221237L3 Lenti ORF clone of Mmp27 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 27 (Mmp27) 10 ug
$813.00
MR221237L4 Lenti ORF clone of Mmp27 (mGFP-tagged) - Mouse matrix metallopeptidase 27 (Mmp27) 10 ug
$813.00

Plasmid Map for MR221237

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR221237
Circular map for MR221237

file-alt Citations

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