Nr2c2 (NM_011630) Mouse Tagged ORF Clone

SKU MR221079

Nr2c2 (Myc-DDK-tagged) - Mouse nuclear receptor subfamily 2, group C, member 2 (Nr2c2)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Nr2c2
• Synonyms: mKIAA4145; TAK1; Tr4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221079 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACCAGCCCCTCCCCGCGCATCCAGATAATTTCCACCGACTCTGCGGTAGCCTCACCTCAGCGCATTC; AGATTGTAACAGACCAGCAGACAGGACAAAAGATCCAGATAGTCACCGCAGTGGATGCCTCTGGATCCTC; TAAACAGCAGTTCATCCTAACCAGCCCAGATGGAGCTGGAACTGGGAAGGTGATCCTGGCTTCTCCGGAA; ACATCCAGTGCCAAGCAGCTCATATTCACCACCTCGGACAACCTTGTCCCTGGCAGGATCCAGATCGTCA; CGGATTCTGCTTCTGTGGAGCGTTTGCTGGGGAAGGCAGACGTCCAGCGGCCCCAGGTGGTGGAGTACTG; TGTGGTCTGTGGCGACAAAGCCTCTGGCCGGCACTATGGGGCTGTCAGTTGTGAAGGTTGCAAAGGTTTC; TTCAAAAGGAGCGTGAGGAAGAATCTGACCTACAGCTGTCGGAGCAGCCAAGACTGCATCATCAACAAGC; ACCACCGTAACCGCTGCCAGTTCTGCCGGCTGAAGAAGTGCCTGGAGATGGGCATGAAAATGGAGTCTGT; ACAGAGTGAACGGAAGCCCTTTGATGTGCAACGGGAGAAACCAAGCAATTGTGCTGCTTCCACTGAGAAG; ATCTATATCCGGAAAGACCTGAGAAGTCCTCTGATAGCCACTCCCACATTTGTGGCAGACAAAGATGGAG; CAAGACAAACAGGTCTTCTTGATCCAGGGATGCTTGTGAACATCCAACAGCCTTTGATACGTGAGGATGG; TACAGTTCTCCTGGCCGCGGATTCCAAGGCTGAAACAAGCCAAGGAGCTCTAGGTACACTGGCAAATGTA; GTGACCTCTTTGGCCAACCTGAGTGAATCTTTGAACAACGGTGATGCTTCAGAAATGCAGCCAGAGGACC; AGTCTGCAAGTGAGATTACTCGGGCATTTGACACCTTAGCGAAAGCACTTAATACCACAGATAGTGCTTC; ACCTCCAAGCCTGGCAGATGGGATAGATGCTAGTGGAGGAGGGAGTATCCATGTCATCAGCAGAGATCAG; TCAACACCCATCATTGAAGTTGAAGGCCCTCTCCTTTCAGACACACATGTCACATTCAAGCTTACAATGC; CCAGTCCTATGCCAGAGTACCTCAATGTACATTACATCTGTGAGTCTGCATCCCGCCTGCTTTTCCTCTC; CATGCACTGGGCAAGGTCAATCCCAGCCTTCCAGGCACTTGGACAGGACTGTAATACCAGCCTGGTGAGG; GCCTGCTGGAATGAGCTCTTCACTCTTGGCCTGGCCCAGTGTGCCCAGGTCATGAGTCTCTCCACCATCC; TGGCAGCCATTGTCAACCACCTACAGAACAGCATCCAGGAAGATAAGCTTTCTGGTGACCGGATAAAGCA; AGTGATGGAGCACATCTGGAAGCTGCAGGAGTTCTGTAACAGCATGGCGAAACTGGATATAGACGGCTAT; GAGTACGCATACCTTAAAGCTATAGTTCTCTTTAGTCCCGATCATCCAGGTTTGACAGGCACAAGCCAGA; TTGAGAAATTTCAGGAGAAGGCACAGATGGAATTACAGGACTATGTGCAGAAAACCTACTCTGAAGATAC; TTACAGATTGGCCAGGATTCTTGTCCGCCTACCAGCACTCAGGCTCATGAGCTCCAACATAACAGAAGAA; CTTTTTTTTACTGGTCTCATTGGCAATGTTTCAATAGACAGCATAATTCCTTACATCCTCAAGATGGAGA; CAGCAGAATATAATGGCCAGATCACTGGAGCCAGTCTA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221079 protein sequence; Red=Cloning site Green=Tags(s); ; MTSPSPRIQIISTDSAVASPQRIQIVTDQQTGQKIQIVTAVDASGSSKQQFILTSPDGAGTGKVILASPE; TSSAKQLIFTTSDNLVPGRIQIVTDSASVERLLGKADVQRPQVVEYCVVCGDKASGRHYGAVSCEGCKGF; FKRSVRKNLTYSCRSSQDCIINKHHRNRCQFCRLKKCLEMGMKMESVQSERKPFDVQREKPSNCAASTEK; IYIRKDLRSPLIATPTFVADKDGARQTGLLDPGMLVNIQQPLIREDGTVLLAADSKAETSQGALGTLANV; VTSLANLSESLNNGDASEMQPEDQSASEITRAFDTLAKALNTTDSASPPSLADGIDASGGGSIHVISRDQ; STPIIEVEGPLLSDTHVTFKLTMPSPMPEYLNVHYICESASRLLFLSMHWARSIPAFQALGQDCNTSLVR; ACWNELFTLGLAQCAQVMSLSTILAAIVNHLQNSIQEDKLSGDRIKQVMEHIWKLQEFCNSMAKLDIDGY; EYAYLKAIVLFSPDHPGLTGTSQIEKFQEKAQMELQDYVQKTYSEDTYRLARILVRLPALRLMSSNITEE; LFFTGLIGNVSIDSIIPYILKMETAEYNGQITGASL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011630
• ORF Size: 1788 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_011630.3, NP_035760.1
• RefSeq Size: 7637 bp
• RefSeq ORF: 1791 bp
• Locus ID: 22026
• UniProt ID: P49117
• Cytogenetics: 6 D1
• MW: 65.2 kDa
• Summary: Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear receptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Activates transcriptional activity of LHCG and is antagonist of PPARA-mediated transactivation (By similarity). Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior.[UniProtKB/Swiss-Prot Function]