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Asic2 (NM_007384) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220993
Asic2 (Myc-DDK-tagged) - Mouse amiloride-sensitive cation channel 1, neuronal (degenerin) (Accn1), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $525.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Asic2
Synonyms Accn1; ACIC2; BNaC1; BNaC1a; BNC1; Mdeg
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220993 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCGGAGCGGCGGAGCCCGGCTGCCGGCGACCGCGCTCAGCGGCCCGGGACGCTTCCGTATGGCCC
GCGAGCAGCCGGCGCCCGCGGCGGTGGCGGCAGCTAGGCAGCCCGGGGGCGACCGGAGCGGCGATCGGGA
GCTGCAGGGGCCAGGGGTCGCCCGCAGGGGGCGGCCGTCCCTGAGTCGCACTAAATTGCACGGGCTGCGG
CACATGTGCGCGGGGCGCACGGCGGCGGGAGGCTCTTTCCAGCGGCGGGCGCTGTGGGTGCTGGCCTTCT
GCACGTCCCTTGGCTTGCTGTTGTCCTGGTCCTCGAACCGCCTGCTCTACTGGCTCAGTTTCCCGTCACA
CACGCGAGTGCACCGCGAGTGGAGCCGCCAGCTGCCGTTCCCCGCTGTCACCGTGTGCAACAACAACCCC
CTGCGCTTCCCGCGCCTCTCCAAGGGGGACCTCTACTACGCGGGCCACTGGCTAGGACTGCTGCTGCCCA
ACCGCACCGCGCGCCCGCTGGTCAGCGAGCTGCTGCGGGGCGACGAGCCGCGCAGACAGTGGTTCCGCAA
GCTGGCCGACTTCCGCCTCTTCCTGCCGCCGCGCCACTTCGAGGGCATCAGCGCTGCCTTCATGGACCGT
CTGGGCCACCAGCTGGAGGACATGCTGCTCTCCTGCAAGTACCGGGGCGAGCTCTGTGGCCCGCACAACT
TCTCCTCAGTGTTTACAAAATATGGGAAGTGTTACATGTTTAACTCAGGCGAGGATGGCAAGCCTCTGCT
CACCACGGTCAAGGGGGGGACGGGCAACGGGCTGGAGATCATGCTGGACATTCAGCAAGATGAGTACCTG
CCCATCTGGGGAGAGACAGAGGAAACAACGTTTGAAGCAGGAGTGAAGGTTCAGATCCACAGCCAGTCTG
AGCCACCTTTCATCCAAGAGCTGGGCTTTGGGGTGGCTCCGGGGTTCCAGACCTTCGTGGCCACACAAGA
GCAGAGGCTCACATATCTGCCCCCGCCGTGGGGGGAGTGCCGGTCCTCAGAGATGGGACTCGACTTCTTT
CCTGTTTACAGCATCACAGCCTGTCGGATCGACTGTGAGACCCGCTACATCGTGGAGAACTGTAACTGCC
GCATGGTCCACATGCCAGGGGATGCCCCTTTCTGCACCCCTGAGCAGCACAAGGAGTGTGCAGAGCCAGC
CCTCGGCTTACTGGCAGAAAAGGACAGCAATTACTGTCTCTGCAGGACACCCTGCAACCTGACCCGCTAC
AACAAAGAGCTCTCCATGGTAAAGATCCCCAGCAAGACGTCAGCCAAGTACCTGGAGAAGAAATTTAACA
AATCGGAAAAATATATCTCCGAGAACATTCTTGTTCTGGATATATTTTTTGAGGCGCTCAATTACGAGAC
AATTGAACAGAAGAAGGCGTATGAAGTTGCTGCCTTACTTGGTGACATTGGTGGTCAAATGGGATTGTTC
ATTGGTGCTAGTATCCTTACAATACTAGAGCTCTTTGATTACATTTACGAGCTGATCAAAGAGAAGCTAT
TAGACCTGCTTGGCAAAGAAGAAGAGGAAGGGAGCCATGATGAGAACATGAGCACCTGCGACACAATGCC
AAACCACTCCGAAACCATCAGCCACACTGTGAACGTGCCCCTGCAGACAGCTTTGGGCACCCTGGAGGAG
ATTGCCTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220993 protein sequence
Red=Cloning site Green=Tags(s)

MSRSGGARLPATALSGPGRFRMAREQPAPAAVAAARQPGGDRSGDRELQGPGVARRGRPSLSRTKLHGLR
HMCAGRTAAGGSFQRRALWVLAFCTSLGLLLSWSSNRLLYWLSFPSHTRVHREWSRQLPFPAVTVCNNNP
LRFPRLSKGDLYYAGHWLGLLLPNRTARPLVSELLRGDEPRRQWFRKLADFRLFLPPRHFEGISAAFMDR
LGHQLEDMLLSCKYRGELCGPHNFSSVFTKYGKCYMFNSGEDGKPLLTTVKGGTGNGLEIMLDIQQDEYL
PIWGETEETTFEAGVKVQIHSQSEPPFIQELGFGVAPGFQTFVATQEQRLTYLPPPWGECRSSEMGLDFF
PVYSITACRIDCETRYIVENCNCRMVHMPGDAPFCTPEQHKECAEPALGLLAEKDSNYCLCRTPCNLTRY
NKELSMVKIPSKTSAKYLEKKFNKSEKYISENILVLDIFFEALNYETIEQKKAYEVAALLGDIGGQMGLF
IGASILTILELFDYIYELIKEKLLDLLGKEEEEGSHDENMSTCDTMPNHSETISHTVNVPLQTALGTLEE
IAC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007384
ORF Size 1689 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007384.3, NP_031410.1
RefSeq Size 3024 bp
RefSeq ORF 1692 bp
Locus ID 11418
UniProt ID Q925H0
Cytogenetics 11 48.43 cM
MW 63.2 kDa
Summary Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_007384" in other vectors (4)
SKU Description Size Price
MC219049 Asic2 (untagged) - Mouse amiloride-sensitive cation channel 1, neuronal (degenerin) (Accn1), transcript variant 1, (10ug) 10 ug
$577.00
MG220993 Asic2 (tGFP-tagged) - Mouse amiloride-sensitive cation channel 1 neuronal (degenerin) (Accn1) transcript variant 1, (10ug) 10 ug
$725.00
MR220993L3 Lenti ORF clone of Asic2 (Myc-DDK-tagged) - Mouse amiloride-sensitive cation channel 1, neuronal (degenerin) (Accn1), transcript variant 1 10 ug
$825.00
MR220993L4 Lenti ORF clone of Asic2 (mGFP-tagged) - Mouse amiloride-sensitive cation channel 1, neuronal (degenerin) (Accn1), transcript variant 1 10 ug
$825.00

Plasmid Map for MR220993

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220993
Circular map for MR220993

file-alt Citations

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