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Aph1a (NM_146134) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220937
Aph1a (Myc-DDK-tagged) - Mouse anterior pharynx defective 1a homolog (C. elegans) (Aph1a), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Aph1a
Synonyms 6530402N02Rik; APH-1a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220937 representing NM_146134
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGCTGCTGTGTTTTTCGGATGCACCTTCGTCGCGTTCGGCCCAGCCTTCTCCCTTTTCCTGATCA
CTGTAGCTGGAGACCCACTTCGGGTTATCATCCTGGTGGCGGGAGCCTTTTTCTGGCTGGTCTCCCTGCT
CTTGGCTTCTGTGGTCTGGTTCATCTTGGTCCATGTGACAGACCGATCAGATGCACGGCTCCAGTATGGC
CTCCTGATTTTTGGTGCTGCTGTCTCTGTCCTTCTACAGGAAGTGTTCCGTTTTGCTTACTACAAGCTCC
TTAAGAAGGCAGATGAGGGCTTAGCATCACTGAGTGAGGACGGAAGATCACCCATCTCCATCCGACAGAT
GGCCTATGTTTCTGGTCTGTCCTTCGGTATCATCAGTGGTGTCTTCTCTGTTATCAATATTTTGGCTGAT
GCACTTGGGCCAGGTGTGGTTGGGATCCATGGAGACTCACCCTATTACTTCCTGACTTCAGCCTTTCTGA
CAGCAGCCATTATCCTGCTCCACACCTTTTGGGGAGTTGTGTTCTTTGATGCCTGTGAGAGGAGACGGTA
CTGGGCTTTGGGCCTGGTAGTTGGGAGTCACCTTCTGACATCGGGACTGACATTCCTGAACCCCTGGTAT
GAGGCTAGCCTGCTGCCCATCTATGCAGTCACCGTTTCCATGGGGCTCTGGGCGTTCATCACAGCCGGAG
GCTCCCTCCGAAGTATCCAGCGCAGCCTTTCGTGCCGCCGGCAGGAGGACAGTCGGGTGATGGTGTACTC
TGCCCTGCGCATCCCACCCGAGGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220937 representing NM_146134
Red=Cloning site Green=Tags(s)

MGAAVFFGCTFVAFGPAFSLFLITVAGDPLRVIILVAGAFFWLVSLLLASVVWFILVHVTDRSDARLQYG
LLIFGAAVSVLLQEVFRFAYYKLLKKADEGLASLSEDGRSPISIRQMAYVSGLSFGIISGVFSVINILAD
ALGPGVVGIHGDSPYYFLTSAFLTAAIILLHTFWGVVFFDACERRRYWALGLVVGSHLLTSGLTFLNPWY
EASLLPIYAVTVSMGLWAFITAGGSLRSIQRSLSCRRQEDSRVMVYSALRIPPED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146134
ORF Size 795 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146134.2, NP_666246.1
RefSeq Size 2882 bp
RefSeq ORF 798 bp
Locus ID 226548
UniProt ID Q8BVF7
Cytogenetics 3 F2.1
MW 29.4 kDa
Summary This gene encodes a subunit of the gamma-secretase complex, which is localized to the endoplasmic reticulum and golgi apparatus. Gamma-secretase is a multi-protein enzyme that catalyzes intramembraneous proteolysis of type I transmembrane proteins and is essential for many signaling pathways, including the Notch signaling pathway. Studies suggest that the protein encoded by this locus binds directly to substrates of the gamma-secretase complex, including the beta-amyloid precursor protein which is associated with Alzheimer disease progression. This gene is required for normal embryonic development and survival, and disruption is associated with defects in the yolk sack angiogenesis, neural tube formation, and somitogenesis. A pseudogene of this gene is located on chromosome 1. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jan 2013
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"NM_146134" in other vectors (3)
SKU Description Size Price
MG220937 Aph1a (tGFP-tagged) - Mouse anterior pharynx defective 1a homolog (C. elegans) (Aph1a) transcript variant 2, (10ug) 10 ug
$500.00
MR220937L3 Lenti ORF clone of Aph1a (Myc-DDK-tagged) - Mouse anterior pharynx defective 1a homolog (C. elegans) (Aph1a), transcript variant 2 10 ug
$600.00
MR220937L4 Lenti ORF clone of Aph1a (mGFP-tagged) - Mouse anterior pharynx defective 1a homolog (C. elegans) (Aph1a), transcript variant 2 10 ug
$600.00

Plasmid Map for MR220937

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220937
Circular map for MR220937

file-alt Citations

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