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Mical3 (NM_153396) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220854
Mical3 (Myc-DDK-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 3 (Mical3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $792.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Mical3 or NM_153396"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mical3
Synonyms C130040D16Rik; Mical-3; mKIAA1364
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220854 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGAGGAAGCAGGAGACAACGAACCAAGCTCACGTCCTCTTTGACCGCTTTGTCCAGGCCACCA
CCTGCAAGGGGACCCTCAGGGCCTTCCAAGAGCTCTGTGACCACCTGGAGCTGAAGCCCAAGGACTACCG
GTCCTTCTACCACAAGCTCAAGTCCAAGCTCAACTACTGGAAGGCCAAAGCCCTCTGGGCCAAATTGGAC
AAGCGGGGCAGTCACAAAGACTACAAAAAGGGCAAAGCCTGCACCAACACAAAGTGTCTCATCATTGGAG
CTGGCCCCTGTGGGCTGCGCACAGCCATCGACTTGTCCTTGTTGGGAGCCAAGGTGGTTGTTATTGAAAA
GCGAGATGCCTTCTCCCGCAACAATGTCTTACATCTCTGGCCCTTCACCATACACGATCTCCGAGGCCTG
GGTGCCAAGAAATTCTATGGCAAGTTCTGTGCCGGAGCCATCGACCATATCAGTATCCGTCAGCTCCAGC
TAATCCTCTTGAAGGTAGCCTTGATCTTGGGCATTGAGATCCATGTCAACGTGGAATTCCAAGGACTTGT
TCAGCCTCCTGAGGACCAAGAGAATGAACGGATAGGATGGCGAGCATTGGTGCACCCCAAAACTCATCCT
GTATCAGAATATGAATTTGAAGTTATCATCGGTGGAGATGGGCGTAGGAACACCTTGGAAGGATTTCGTC
GAAAAGAATTTCGTGGCAAGCTAGCCATTGCCATTACAGCAAATTTTATCAACCGCAACACAACCGCAGA
GGCCAAAGTGGAGGAGATTAGTGGCGTGGCTTTTATATTCAACCAAAAATTTTTCCAGGAGCTGAGAGAA
GCCACAGGTATTGACTTAGAGAACATCGTCTACTACAAAGATGACACACACTACTTTGTCATGACGGCCA
AAAAGCAGAGTTTGCTGGACAAGGGGGTGATCCTGCATGACTACACAGACACGGAGCTGTTGCTTTCCCG
GGAGAACGTGGACCAGGAGGCCCTGCTGAACTATGCCCGGGAGGCCGCAGACTTCTCTACCCAGCAGCAG
CTGCCTTCTCTGGATTTCGCCATCAATCACTACGGGCAGCCAGACGTGGCCATGTTTGACTTTACCTGCA
TGTACGCCTCTGAAAATGCCGCCTTGGTCCGGGAACAGAACGGACACCAGTTACTAGTAGCTTTGGTTGG
AGACAGCCTCCTGGAGCCTTTCTGGCCAATGGGGACAGGAATAGCCCGGGGCTTTCTGGCTGCTATGGAC
TCTGCCTGGATGGTACGAAGCTGGTCTCTGGGCACCAGCCCCTTGGAAGTCCTGGCAGAAAGGGAAAGCA
TTTATAGGTTGCTGCCTCAGACCACCCCTGAGAACGTGAGCAAAAACTTCAGCCAATATAGCATCGACCC
TGTCACTAGGTATCCCAATATCAACATCAACTTCCTCAGGCCAAGCCAGGTGCGCCATTTATACGATAGC
GGAGAAACAAAAGATATCCACCTGGAAATGGAGAACATGGTGAATCCCCGAACCACTCCCAAGCTGACTC
GCAACGAGTCTGTAGCTCGTTCAAGCAAACTGCTGGGGTGGTGCCAAAGGCAGACAGAAGGCTATTCAGG
AGTCAATGTGACAGATCTCACTATGTCTTGGAAAAGTGGCCTGGCCCTGTGTGCGATCATCCACAGATAC
CGCCCCGATCTAATAGACTTTGATTCTTTGGATGAGCAAAACGTGGAGAAGAATAATCAGCTGGCCTTTG
ACATCGCTGAGAAGGAACTGGGTATCTCTCCCATCATGACGGGCAAGGAGATGGCTTCGGTCGGGGAGCC
AGACAAGCTGTCCATGGTGATGTACCTCACGCAGTTCTACGAGATGTTCAAGGACTCGCTCTCCTCCAGC
GACACCCTCGACCTGAATGCAGAGGAGAAAGCCGTCCTGATAGCCAGCACCAAATCCCCCATCTCCTTCC
TGAGCAAACTCGGACAGACCATCTCTCGGAAGCGTTCGCCCAAGGATAAAAAAGAAAAGGACTCAGATGG
GGCTGGAAAGAGGAGAAAAACCAGTCAGTCAGAGGAGGAGGAGCCTCCCCGGAGCTACAAAGGAGAAAGA
CCGACCCTGGTGAGCACCCTGACGGACAGACGGATGGATGCTGCCGTTGGGAACCAGAACAAAGTGAAGT
ACATGGCGACCCAGCTGCTGGCCAAGTTTGAAGAGAACGCACCTGCTCAGTCCACTGGTGTGAGGAGACA
GGGCTCCATAAAGAAAGAGTTCCCGCAGAACTTGGGGGGCAGCGACACGTGCTATTTCTGCCAAAAGCGG
GTCTACGTGATGGAGAGGCTGAGCGCCGAGGGCAAGTTCTTCCATCGAAGCTGCTTCAAGTGTGAGTACT
GTGCCACCACGCTGCGCCTATCAGCCTACGCCTACGACATCGAGGATGAGTTCTCTCCAAACTTCTGGAC
ATCAGCCAGCTACCATGTTCCAGTTGCCCTTCCTGCTACGGTCATGCCTATGTGCCTTCTGTACCATCCA
TCTCAAGTGCTCGTCTGCCTCGAGGGAGGCCCAGCATTCATGTCACCAGTGTTATTCAATGACACCAACA
GT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220854 protein sequence
Red=Cloning site Green=Tags(s)

MEERKQETTNQAHVLFDRFVQATTCKGTLRAFQELCDHLELKPKDYRSFYHKLKSKLNYWKAKALWAKLD
KRGSHKDYKKGKACTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGL
GAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFQGLVQPPEDQENERIGWRALVHPKTHP
VSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELRE
ATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYTDTELLLSRENVDQEALLNYAREAADFSTQQQ
LPSLDFAINHYGQPDVAMFDFTCMYASENAALVREQNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMD
SAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNININFLRPSQVRHLYDS
GETKDIHLEMENMVNPRTTPKLTRNESVARSSKLLGWCQRQTEGYSGVNVTDLTMSWKSGLALCAIIHRY
RPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGKEMASVGEPDKLSMVMYLTQFYEMFKDSLSSS
DTLDLNAEEKAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDSDGAGKRRKTSQSEEEEPPRSYKGER
PTLVSTLTDRRMDAAVGNQNKVKYMATQLLAKFEENAPAQSTGVRRQGSIKKEFPQNLGGSDTCYFCQKR
VYVMERLSAEGKFFHRSCFKCEYCATTLRLSAYAYDIEDEFSPNFWTSASYHVPVALPATVMPMCLLYHP
SQVLVCLEGGPAFMSPVLFNDTNS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153396
ORF Size 2592 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153396.1, NM_153396.2, NM_153396.3, NP_700445.2
RefSeq Size 2929 bp
RefSeq ORF 2595 bp
Locus ID 194401
UniProt ID Q8CJ19
Cytogenetics 6 F1
MW 97.9 kDa
Summary Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization. In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2). Seems to act as Rab effector protein and play a role in vesicle trafficking. Involved in exocytic vesicles tethering and fusion: the monooxygenase activity is required for this process and implicates RAB8A associated with exocytotic vesicles. Required for cytokinesis. Contributes to stabilization and/or maturation of the intercellular bridge independently of its monooxygenase activity. Promotes recruitment of Rab8 and ERC1 to the intercellular bridge, and together these proteins are proposed to function in timely abscission.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_153396" in other vectors (4)
SKU Description Size Price
MC222284 Mical3 (untagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 3 (Mical3), (10ug) 10 ug
$871.00
MG220854 Mical3 (tGFP-tagged) - Mouse microtubule associated monoxygenase calponin and LIM domain containing 3 (Mical3), (10ug) 10 ug
$992.00
MR220854L3 Lenti ORF clone of Mical3 (Myc-DDK-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 3 (Mical3) 10 ug
$1,092.00
MR220854L4 Lenti ORF clone of Mical3 (mGFP-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 3 (Mical3) 10 ug
$1,092.00

Plasmid Map for MR220854

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220854
Circular map for MR220854

file-alt Citations

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