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Mical3 (NM_153396) Mouse Untagged Clone

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SKU
MC222284
Mical3 (untagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 3 (Mical3), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $871.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Mical3
Synonyms C130040D16Rik; Mical-3; mKIAA1364
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC222284 representing NM_153396
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGAGGAAGCAGGAGACAACGAACCAAGCTCACGTCCTCTTTGACCGCTTTGTCCAGGCCACCA
CCTGCAAGGGGACCCTCAGGGCCTTCCAAGAGCTCTGTGACCACCTGGAGCTGAAGCCCAAGGACTACCG
GTCCTTCTACCACAAGCTCAAGTCCAAGCTCAACTACTGGAAGGCCAAAGCCCTCTGGGCCAAATTGGAC
AAGCGGGGCAGTCACAAAGACTACAAAAAGGGCAAAGCCTGCACCAACACAAAGTGTCTCATCATTGGAG
CTGGCCCCTGTGGGCTGCGCACAGCCATCGACTTGTCCTTGTTGGGAGCCAAGGTGGTTGTTATTGAAAA
GCGAGATGCCTTCTCCCGCAACAATGTCTTACATCTCTGGCCCTTCACCATACACGATCTCCGAGGCCTG
GGTGCCAAGAAATTCTACGGCAAGTTCTGTGCCGGAGCCATCGACCATATCAGTATCCGTCAGCTCCAGC
TAATCCTCTTGAAGGTAGCCTTGATCTTGGGCATTGAGATCCATGTCAATGTGGAATTCCAAGGACTTGT
TCAGCCTCCCGAGGACCAAGAGAATGAACGGATAGGATGGCGAGCATTGGTGCACCCCAAAACTCATCCT
GTATCAGAATATGAATTTGAAGTTATCATCGGTGGAGATGGGCGTAGGAACACCTTGGAAGGATTTCGTC
GAAAAGAATTTCGTGGCAAGCTAGCCATTGCCATTACAGCAAATTTTATCAACCGCAACACAACCGCAGA
GGCCAAAGTGGAGGAGATTAGTGGCGTGGCTTTTATATTCAACCAAAAATTTTTCCAGGAGCTGAGAGAA
GCCACAGGTATTGACTTAGAGAACATCGTCTACTACAAAGATGACACACACTACTTTGTCATGACGGCCA
AAAAGCAGAGTTTGCTGGACAAGGGGGTGATCCTGCATGACTACACAGACACGGAGCTGTTGCTTTCCCG
GGAGAACGTGGACCAGGAGGCCCTGCTGAACTATGCCCGGGAGGCCGCAGACTTCTCTACCCAGCAGCAG
CTGCCTTCTCTGGATTTCGCCATCAATCACTACGGGCAGCCAGACGTGGCCATGTTTGACTTTACCTGCA
TGTACGCCTCTGAAAATGCCGCCTTGGTCCGGGAACAGAACGGACACCAGTTACTAGTAGCTTTGGTTGG
AGACAGCCTCCTGGAGCCTTTCTGGCCAATGGGGACAGGAATAGCCCGGGGCTTTCTGGCTGCTATGGAC
TCTGCCTGGATGGTACGAAGCTGGTCTCTGGGCACCAGCCCCTTGGAAGTCCTGGCAGAAAGGGAAAGCA
TTTATAGGTTGCTGCCTCAGACCACCCCTGAGAACGTGAGCAAAAACTTCAGCCAATATAGCATCGACCC
TGTCACTAGGTATCCCAATATCAACATCAACTTCCTCAGGCCAAGCCAGGTGCGCCATTTATACGATAGC
GGAGAAACAAAAGATATCCACCTGGAAATGGAGAACATGGTGAATCCCCGAACCACTCCCAAGCTGACTC
GCAACGAGTCTGTAGCTCGTTCAAGCAAACTGCTGGGGTGGTGCCAAAGGCAGACAGAAGGCTATTCAGG
AGTCAATGTGACAGATCTCACTATGTCTTGGAAAAGTGGCCTGGCCCTGTGTGCGATCATCCACAGATAC
CGCCCCGATCTAATAGACTTTGATTCTTTGGATGAGCAAAACGTGGAGAAGAATAATCAGCTGGCCTTTG
ACATCGCTGAGAAGGAACTGGGTATCTCTCCCATCATGACGGGCAAGGAGATGGCTTCGGTCGGGGAGCC
AGACAAGCTGTCCATGGTGATGTACCTCACGCAGTTCTACGAGATGTTCAAGGACTCGCTCTCCTCCAGC
GACACCCTCGACCTGAATGCAGAGGAGAAAGCCGTCCTGATAGCCAGCACCAAATCCCCCATCTCCTTCC
TGAGCAAACTCGGACAGACCATCTCTCGGAAGCGTTCGCCCAAGGATAAAAAAGAAAAGGACTCAGATGG
GGCTGGAAAGAGGAGAAAAACCAGTCAGTCAGAGGAGGAGGAGCCTCCCCGGAGCTACAAAGGAGAAAGA
CCGACCCTGGTGAGCACCCTGACGGACAGACGGATGGATGCTGCCGTTGGGAACCAGAACAAAGTGAAGT
ACATGGCGACCCAGCTGCTGGCCAAGTTTGAAGAGAACGCACCTGCTCAGTCCACTGGTGTGAGGAGACA
GGGCTCCATAAAGAAAGAGTTCCCGCAGAACTTGGGGGGCAGCGACACGTGCTATTTCTGCCAAAAGCGG
GTCTACGTGATGGAGAGGCTGAGCGCCGAGGGCAAGTTCTTCCATCGAAGCTGCTTCAAGTGTGAGTACT
GTGCCACCACGCTGCGCCTATCAGCCTACGCCTACGACATCGAGGATGAGTTCTCTCCAAACTTCTGGAC
ATCAGCCAGCTACCATGTTCCAGTTGCCCTTCCTGCTACGGTCATGCCTATGTGCCTTCTGTACCATCCA
TCTCAAGTGCTCGTCTGCCTCGAGGGAGGCCCAGCATTCATGTCACCAGTGTTATTCAATGACACCAACA
GTTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_153396
Insert Size 2595 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153396.3, NP_700445.2
RefSeq Size 2929 bp
RefSeq ORF 2595 bp
Locus ID 194401
UniProt ID Q8CJ19
Cytogenetics 6 F1
Summary Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization. In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2). Seems to act as Rab effector protein and play a role in vesicle trafficking. Involved in exocytic vesicles tethering and fusion: the monooxygenase activity is required for this process and implicates RAB8A associated with exocytotic vesicles. Required for cytokinesis. Contributes to stabilization and/or maturation of the intercellular bridge independently of its monooxygenase activity. Promotes recruitment of Rab8 and ERC1 to the intercellular bridge, and together these proteins are proposed to function in timely abscission.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) differs in the 5' UTR, lacks several 3' exons and includes an alternate 3' exon. The encoded isoform (2) has a distinct and shorter C-terminus compared to isoform 1.
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Documents
Resources
"NM_153396" in other vectors (4)
SKU Description Size Price
MG220854 Mical3 (tGFP-tagged) - Mouse microtubule associated monoxygenase calponin and LIM domain containing 3 (Mical3), (10ug) 10 ug
$992.00
MR220854 Mical3 (Myc-DDK-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 3 (Mical3) 10 ug
$589.00 $792.00
MR220854L3 Lenti ORF clone of Mical3 (Myc-DDK-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 3 (Mical3) 10 ug
$1,092.00
MR220854L4 Lenti ORF clone of Mical3 (mGFP-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 3 (Mical3) 10 ug
$1,092.00

file-alt Citations

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