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Dcst1 (NM_029974) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220776
Dcst1 (Myc-DDK-tagged) - Mouse DC-STAMP domain containing 1 (Dcst1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $670.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dcst1
Synonyms A330106H01Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220776 representing NM_029974
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTTCCTCTCATCAACACTTCACAGCCTAGGGATCTTTGAAAAGATTTCCAGGATAAAAGAGGTTC
TCAAGAATAGGCTGCTGGACCTTACAAAGCGTCGGGACCAAGCACGGGAGCAACAAAGGAAACGGCCCCA
CACCATCATACAGGGGCTGCTCCTCTGGAGTCTTCCAGTTTCCTGGATCCGGTTCCTGTGGCGACAGCCA
GGGGAGTTTCCAGTAACTGCTTTCCTGCTAGGAGCAGGCACTGGTGGGCTCCTGGCCATAGGTCTCTTTC
AGCTCTTGGTGAATCCCATGAACATTTATGAGGAGCAGAAGGTGGTGGCGTTATACTGCTTGGCAAGCTT
GGGGGCCATCGGCTGGGGCACCTCCCCTCACATCCGCTGTGCCAGTCTCCTGTTGGTACCCAAGATGCTG
GGCAAAGAAGGCAGACTCTTTGTCATGGGATATGCTTTGGCTGCTATCTACAGTGGGCCGGCGGCCAATC
TAAGGTCCAACATCAACGAGGTGATAGCATCCCTGGGCTGCACTGTGGAGCTGCAGATCAACAACACCCG
ATCCGCCTGGCGAGTCTCCACAGCCCCTCTGCGGGCAGTGTTCAAGGGCATGGTGGGCAGCAAAGATTCA
TTGAATAAAGAGATTCAGAACGTCTCTACCTCCTTTGAAGAGATGGATGAACAGGTGAAGAGTGATGCTG
GCTACTCATCTGAGGACTGGGACAAGAACAGAGAAAGCACAGAGATGTTCGGAACCTCCCGCGTCCGACC
CTACCTGTCCACACAGATGGTGTATGAGCTGAGGACCAGGCTACGCTGCATCCATGTAGTAAATAAGGCC
ATACTCAGCTGCTACCGCTGGTTTGATAAAAAGCATAAAAACTGCATGCGACGCATCCACCTCCCACTCT
TCAACAACATGATCTGTGTGCCAATGAAGTTCAAGTTCCTCTGTAACATTGCCAAGGTGATAGAGATCTG
GTGTTACAAACGCATCCCCGTGGAAGGCAACTTTGGGCAAACCTATGACTCGGTCAACCAGTCTATCCAT
GGACTGTCTGGAGAATTTTCAGCCAATATTAACCTTAAGGAAGAGAAGCAGTCTTCAATGGTGGGTCTCA
ACACCACGAACTGGGAGCACATGGGCACCGAGGTGCGGGACTATGTTCGCCAGCAGGAGACCTATCTGCA
GTGGGCTATGGGGCTGCTGCACGTGCTTCTGTCCTGCACGTTCCTGCTGGTCTTCCATTCAGCCTTCTCC
TACATGGACCACTACAACTGGGACATCCGCTTTGACAACATCTACATCAGCACCTACTTCTGTCAGATTG
ATGCACGCAGGAAGAAGCTGGGCAAACAGAGTCTGTTGCCACTCCGCAAGGCAGAAAGGAAAACGGTTAT
CTTCCCTTTCAAAGCTACTATCCAGGCCTGGGAAATGCGCTATGTGATAAGAGAGCTCCTAGAGACTCTG
CCTATTGTGCTGCTGCTCCTGGTGCTGTGCGCGATCGACTGGGCACTCTACTCTGTCTTTGACACCATCC
GCCAGCACTCTTTCGTGCAGTATTCCTTTCGAAGCAGTCACAAGCTGGAGATAAAAGTCGAGGGAGATTC
CATACTCGCCAAGCTTCTTCGTAAAACCATCGGCGCTCTGAACACCTCCTCGAGTACAGATGTGGAAACA
AACAACATGCCCTGCCTGCCCCAACCCATTAGCCTGAATGCCAGGGATTACTTCAAGGCCTCACTGCCAA
CCCTCCTGCTTGTGTGTCTCTGCCTAGCTCAGGCCTTTGGCTACCGACTCCGGAGAGTCATCGCAGCCTT
CTATTTCCCCAAGCGAGAGAAGAAGCGGGCTCTGTTCTTCTACAATGAGTTCCTGAAGAAGAGATCAGCT
TTCACTAAACTGAGGAGGGCCGCCATTGTTCGGAGGGCAAATCAGCAGAAGGCTCCACCCCACTACCTGG
TGGAGGCTCTGTATCGCCGGTGCCCTCTCCTGCACCGCTTCATGCGTCAGCGCTGTGTGGTGTGCCAGGC
AATGGAGACGCCGGATTCCTACGTGTGCCCCACACCAGACTGCAAGGCCCTGTACTGCCGGTCGTGCTGG
GACGACATGCAACGGCTCTGCCCGGTGTGCACGCCCCGCGAAGAGCTCTCGTCATCCGCCCACAGCGACA
GCAACGACGACGCTGTGTATGGCGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220776 representing NM_029974
Red=Cloning site Green=Tags(s)

MAFLSSTLHSLGIFEKISRIKEVLKNRLLDLTKRRDQAREQQRKRPHTIIQGLLLWSLPVSWIRFLWRQP
GEFPVTAFLLGAGTGGLLAIGLFQLLVNPMNIYEEQKVVALYCLASLGAIGWGTSPHIRCASLLLVPKML
GKEGRLFVMGYALAAIYSGPAANLRSNINEVIASLGCTVELQINNTRSAWRVSTAPLRAVFKGMVGSKDS
LNKEIQNVSTSFEEMDEQVKSDAGYSSEDWDKNRESTEMFGTSRVRPYLSTQMVYELRTRLRCIHVVNKA
ILSCYRWFDKKHKNCMRRIHLPLFNNMICVPMKFKFLCNIAKVIEIWCYKRIPVEGNFGQTYDSVNQSIH
GLSGEFSANINLKEEKQSSMVGLNTTNWEHMGTEVRDYVRQQETYLQWAMGLLHVLLSCTFLLVFHSAFS
YMDHYNWDIRFDNIYISTYFCQIDARRKKLGKQSLLPLRKAERKTVIFPFKATIQAWEMRYVIRELLETL
PIVLLLLVLCAIDWALYSVFDTIRQHSFVQYSFRSSHKLEIKVEGDSILAKLLRKTIGALNTSSSTDVET
NNMPCLPQPISLNARDYFKASLPTLLLVCLCLAQAFGYRLRRVIAAFYFPKREKKRALFFYNEFLKKRSA
FTKLRRAAIVRRANQQKAPPHYLVEALYRRCPLLHRFMRQRCVVCQAMETPDSYVCPTPDCKALYCRSCW
DDMQRLCPVCTPREELSSSAHSDSNDDAVYGD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029974
ORF Size 2196 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029974.2, NP_084250.1
RefSeq Size 2312 bp
RefSeq ORF 2199 bp
Locus ID 77772
UniProt ID Q059Y8
Cytogenetics 3 F1
MW 84.8 kDa
Summary This gene encodes a transmembrane protein. A similar gene in human has been associated with dendritic cells. provided by RefSeq, May 2015
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"NM_029974" in other vectors (4)
SKU Description Size Price
MC221185 Dcst1 (untagged) - Mouse DC-STAMP domain containing 1 (Dcst1), (10ug) 10 ug
$738.00
MG220776 Dcst1 (tGFP-tagged) - Mouse DC-STAMP domain containing 1 (Dcst1), (10ug) 10 ug
$870.00
MR220776L3 Lenti ORF clone of Dcst1 (Myc-DDK-tagged) - Mouse DC-STAMP domain containing 1 (Dcst1) 10 ug
$970.00
MR220776L4 Lenti ORF clone of Dcst1 (mGFP-tagged) - Mouse DC-STAMP domain containing 1 (Dcst1) 10 ug
$970.00

Plasmid Map for MR220776

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220776
Circular map for MR220776

file-alt Citations

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