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Dsg3 (NM_030596) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220716
Dsg3 (Myc-DDK-tagged) - Mouse desmoglein 3 (Dsg3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $909.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dsg3
Synonyms bal
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220716 representing NM_030596
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCTGCCTCTTCCCTAGAGCCTTGGGGTCTCTGGCCCTCCTGATGGTGGTGCTGCTGGTACAGGGGG
AACTGCATGTGAAGCCGGGAGGACAGCACAGAGAAGATGGGACAGCATTGCAGCTGGCCAAAAGAAGGTA
CAAACGTGAATGGGTGAAATTTGCAAAACCCTGCAGAGAAAGAGAAGACAACTCAAGACGAAACCCAATT
GCCAAGATCACTTCAGATTTCCAAAAAAACCAGAAAATTACCTACCGCATTTCTGGAGTGGGAATTGATC
AGCCTCCCTTTGGGATCTTTGTTGTTGACCCAAACAATGGTGATATCAACATAACAGCCATAGTTGATCG
TGAGGAAACCCCAAGCTTCCTGATCACATGCCGTGCACTAAATGCCCTGGGACAGGATGTAGAGAGACCA
CTTATATTAACAGTGAAAATTTTAGACGTCAATGATAACCCTCCAATATTTTCACAAACCATATTCAAGG
GTGAAATTGAAGAAAATAGTGCTTCAAATTCCCTGGTAATGATCCTAAATGCCACGGATGCAGATGAACC
AAACCATATGAACTCTAAAATTGCCTTCAAAATTGTCTCCCAGGAGCCCGCAGGCATGTCCATGTTCCTC
ATCAGCAGAAACACCGGAGAAGTCCGCACTTTGACCAGTTCTCTTGATCGAGAGCAAATTAGTAGCTACC
ATCTGGTTGTGAGCGGTGCAGACAACGATGGAACAGGACTGTCAACTCAGTGTGAATGTAGTATTAAAAT
TAAAGATGTCAATGACAACTTCCCAGTGCTCAGAGAGTCTCAGTATTCAGCACGTATTGAAGAAAATACT
TTGAATGCAGAGCTACTCCGATTTCAAGTGACAGACTGGGATGAAGAATACACAGATAATTGGTTGGCTG
TGTATTTCTTCACCTCTGGGAATGAGGGGAATTGGTTTGAAATTGAAACAGATCCTAGAACCAATGAAGG
CATCCTGAAGGTGGTTAAGGCTCTGGATTATGAACAAGTGCAGAGTATGCAGTTTAGTATTGCTGTCAGA
AACAAAGCTGAGTTCCACCAGTCAGTGATCTCTCAGTACCGAGTGCAGTCAACGCCAGTCACAATTCAAG
TCATTGATGTCCGAGAGGGAATCTCATTCCGTCCTCCTTCCAAGACATTTACTGTGCAAAGGGGTGTAAG
CACTAACAAATTAGTTGGTTACATCCTGGGGACATACCAAGCTACTGATGAGGACACGGGTAAAGCTGCC
TCCTCTGTCAGATATGTCCTGGGTCGTAATGATGGTGGTTTGCTTGTTATTGATTCAAAGACTGCTCAGA
TCAAATTTGTCAAAAACATCGACCGAGATTCTACTTTCATAGTTAACAAGACAATTTCTGCTGAGGTTCT
GGCCATAGACGAAAACACTGGTAAGACCTCCACAGGCACAATATATGTGGAAGTGCCCAGTTTTAATGAA
AACTGCCCGTCAGTTGTCCTCGAGAAGAAAGACATCTGTACTTCCTCACCTTCTGTGACCCTCTCAGTGA
GAACCCTGGACAGGGGTAAATACACTGGCCCCTACACAGTGTCCCTGGAGGAGCAGCCACTGAAGCTGCC
AGTCATGTGGACCATCACGACGCTGAATGCTACATCAGCTCTTCTACAAGCCCAGCAGCAAGTGTCTCCA
GGAGTGTACAATGTCCCAGTCATCGTGAAGGACAATCAGGATGGGCTGTGTGACACACCAGAGAGCTTGA
CCCTGACAGTGTGTCAATGTGATGACCGGAGCATGTGCAGAGCCCCTATCCCGAGCAGAGAGCCTAACAC
ATATGGGGAGTCATCCTGGAGGCTGGGGCCTGCTGCCATTGGCTTGATCCTGCTAGGACTCCTGATGTTG
CTATTGGCCCCACTTCTGCTTCTGACCTGTGACTGTGGGTCAGGTCCCATTGGAGGAGCAGCGACAGGTG
GCTTTATCCCAGTGCCCGATGGTTCGGAAGGTACAATTCATCAGTGGGGAATCGAAGGAGCTCAGCCCGA
AGACAAGGAAATCACAAATATTTGTGTTCCTCCAGTTACAACCAATGGAGCCGATTTTATGGAAAGCTCT
GAAGTTTGCACAAATACATATGCTGGTGGGACCATGGTAGAAGGTGCTTCAGGAATGGAAATGATTACCA
AGCTGGGAGGAGCTACTGGAGCTACAGCGGCACTTGGTCCCTGTTCCTTGGGTTATTCAGGAACAATGAG
AACAAGGCATTCCACTGGAGGGACCTTGAAGGACTATGCTGCGCCAGTCAACATGACTTTCCTAGGCTCC
TATTTCTCTCAGAAATCACTCGCCTATGCCGAGGAAGAGGATGAGCGCGAAGTGAACGACTGTTTGCTGA
TCTACGATGATGAAGGCGAGGATGCTGCCCCCCACTCCCCCACCCTCAGCTCCTGCAGCATTTTTGCCGA
TGACCTAGACGACAACTTCTTGGATTCCCTTGGCCCCAAATTTAAAAAGCTTGCAGAGATCTGTCTCGGT
ATTGATGACGAAGCCAAGCAAGCAAAGCCAGGCCCTAAAGACAGTGGTTCTGGGGCTGATACCTGCGCTC
GCTCTATGGAAGTCCCTCAGTCAGGTTCTAACAGGTACCAGACTTTGCCTGGCAGTCTGGAAGTCACCCA
AACGGGTTCTAAAATATGCCACACGCTGTCAGGCAACCAAGAAACTTCTGTTATGTCCACTTCTGGGTCT
GTCCACCCTGCTGTTGCCATCCCTGACCCTCTACAGCTTGGGAACTACTTGCTCACAGAAACTTACTCAA
CTTCAGGTTCCTTTGCACAACCCACCACTGTTACCTTTGACCCCCATGTCACACAAAATGTCACTGTAAC
AGAAAGGGTCATTTGTCCCCTTCCTAGTGCCTCCAGCAGTATAGTGGCTCCCACAGAACTGCGAGGCTCA
TACAATATGCTCTATACAAAAGAAACCTGTTCCCATCTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220716 representing NM_030596
Red=Cloning site Green=Tags(s)

MTCLFPRALGSLALLMVVLLVQGELHVKPGGQHREDGTALQLAKRRYKREWVKFAKPCREREDNSRRNPI
AKITSDFQKNQKITYRISGVGIDQPPFGIFVVDPNNGDINITAIVDREETPSFLITCRALNALGQDVERP
LILTVKILDVNDNPPIFSQTIFKGEIEENSASNSLVMILNATDADEPNHMNSKIAFKIVSQEPAGMSMFL
ISRNTGEVRTLTSSLDREQISSYHLVVSGADNDGTGLSTQCECSIKIKDVNDNFPVLRESQYSARIEENT
LNAELLRFQVTDWDEEYTDNWLAVYFFTSGNEGNWFEIETDPRTNEGILKVVKALDYEQVQSMQFSIAVR
NKAEFHQSVISQYRVQSTPVTIQVIDVREGISFRPPSKTFTVQRGVSTNKLVGYILGTYQATDEDTGKAA
SSVRYVLGRNDGGLLVIDSKTAQIKFVKNIDRDSTFIVNKTISAEVLAIDENTGKTSTGTIYVEVPSFNE
NCPSVVLEKKDICTSSPSVTLSVRTLDRGKYTGPYTVSLEEQPLKLPVMWTITTLNATSALLQAQQQVSP
GVYNVPVIVKDNQDGLCDTPESLTLTVCQCDDRSMCRAPIPSREPNTYGESSWRLGPAAIGLILLGLLML
LLAPLLLLTCDCGSGPIGGAATGGFIPVPDGSEGTIHQWGIEGAQPEDKEITNICVPPVTTNGADFMESS
EVCTNTYAGGTMVEGASGMEMITKLGGATGATAALGPCSLGYSGTMRTRHSTGGTLKDYAAPVNMTFLGS
YFSQKSLAYAEEEDEREVNDCLLIYDDEGEDAAPHSPTLSSCSIFADDLDDNFLDSLGPKFKKLAEICLG
IDDEAKQAKPGPKDSGSGADTCARSMEVPQSGSNRYQTLPGSLEVTQTGSKICHTLSGNQETSVMSTSGS
VHPAVAIPDPLQLGNYLLTETYSTSGSFAQPTTVTFDPHVTQNVTVTERVICPLPSASSSIVAPTELRGS
YNMLYTKETCSHL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030596
ORF Size 2979 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030596.4, NP_085099.2
RefSeq Size 4108 bp
RefSeq ORF 2982 bp
Locus ID 13512
UniProt ID O35902
Cytogenetics 18 11.39 cM
MW 107.9 kDa
Summary This gene encodes a member of the cadherin family of proteins that forms an integral transmembrane component of desmosomes, the multiprotein complexes involved in cell adhesion, organization of the cytoskeleton, cell sorting and cell signaling. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. Mice lacking the encoded protein exhibit loss of keratinocyte cell adhesion resulting in a phenotype that resembles that of patients with pemphigus vulgaris. This gene is located in a cluster of desmosomal cadherin genes on chromosome 18. provided by RefSeq, Feb 2016
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Documents
Resources
"NM_030596" in other vectors (4)
SKU Description Size Price
MC222988 Dsg3 (untagged) - Mouse desmoglein 3 (Dsg3), (10ug) 10 ug
$1,001.00
MG220716 Dsg3 (tGFP-tagged) - Mouse desmoglein 3 (Dsg3), (10ug) 10 ug
$1,109.00
MR220716L3 Lenti ORF clone of Dsg3 (Myc-DDK-tagged) - Mouse desmoglein 3 (Dsg3) 10 ug
$1,209.00
MR220716L4 Lenti ORF clone of Dsg3 (mGFP-tagged) - Mouse desmoglein 3 (Dsg3) 10 ug
$1,209.00

Plasmid Map for MR220716

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220716
Circular map for MR220716

file-alt Citations

Regulatory T-cell dysfunction induces autoantibodies to bullous pemphigoid antigens in mice and human subjects
Muramatsu, Ujiie, Kobayashi et al
J Allergy Clin Immunol (2018) 142 (6), 1818-1830.e6 DOI: 10.1016/j.jaci.2018.03.014
Cited by 89
AI Summary: In this study, OriGene products—specifically, the anti-BP230 antibody (Cat# TA324964) and anti-COL17 antibody (Cat# TA327025)—were used to identify and analyze autoantibodies targeting BP230 and COL17 in mouse and human samples, helping to elucidate the role of regulatory T-cell dysfunction in autoimmune skin disease.
制御性 T 細胞の機能不全はマウスとヒトにおいて類天疱瘡抗原への自己抗体産生を誘導する
村松憲
Thesis (2019)
Snippet: マウス COL17 全長リコンビナントタンパク，マウス COL17NC14A ドメ インは過去に当 究室で作成したものを使用した (Nishie et al., 2015;Toyonaga et al., 2017)．マウス desmoglein3(DSG3)リコンビナントタンパクは マウス Desmoglein3 ベクター(MR220716，Origene
Showing 1-2 of 2 papers.
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