Mrgprh (NM_030726) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR220655
Mrgprh (Myc-DDK-tagged) - Mouse MAS-related GPR, member H (Mrgprh)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mrgprh
Synonyms Gpr90; Mgrb; MrgH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220655 representing NM_030726
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCATTGGCAATGACCTTGTATCCTCTGGAATCCACACAACCCACCAGAAACAAAACCCCAAATG
AAACCACCTGGTCTTCAGAGCATACGGATGACCACACTTACTTCTTAGTCTCCCTGGTCATTTGTTCCCT
GGGACTGGCTGGGAATGGCCTTTTGATATGGTTCCTAATCTTCTGTATCAAGAGGAAGCCATTCACCATC
TACATCCTCCATCTCGCCATTGCAGACTTCATGGTCCTCCTCTGTTCGTCCATCATGAAGCTAGTGAACA
CTTTTCACATCTACAATATGACCTTAGAGAGCTACGCCATCCTCTTCATGATCTTTGGCTACAACACAGG
GCTCCACCTCCTCACGGCCATCAGCGTAGAGCGGTGCCTCTCCGTGCTTTATCCAATCTGGTACCAGTGC
CAACGCCCAAAACACCAGTCCGCTGTGGCCTGTATGCTGCTGTGGGCCCTCTCTGTTCTCGTGTCTGGTT
TGGAAAACTTCTTCTGCATTCTGGAAGTGAAGCCCCAATTCCCAGAATGCCGATACGTGTACATATTCTC
CTGTATCTTGACTTTCCTGGTCTTCGTTCCTCTCATGATCTTCTCTAATTTGATCCTCTTCATCCAAGTC
TGCTGTAACCTGAAGCCACGTCAACCGACCAAGCTCTATGTGATCATCATGACCACGGTCATCCTGTTTC
TTGTCTTCGCCATGCCCATGAAGGTGTTGCTTATCATCGGCTACTATTCTAGTTCTCTTGATGATTCTGT
ATGGGACTCCCTCCCCTACCTGAACATGCTGTCCACTATAAACTGCAGCATCAACCCAATTGTCTACTTT
GTGGTAGGCAGCCTGAGGAGGAAGAGGAGTAGGAAGTCCCTAAAAGAAGCACTGCAGAAAGTCTTCGAGG
AAAAGCCAGTGGTGGCCTCGAGGGAGAATGTGACACAGTTCTCGCTGCCTTCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR220655 representing NM_030726
Red=Cloning site Green=Tags(s)

MEPLAMTLYPLESTQPTRNKTPNETTWSSEHTDDHTYFLVSLVICSLGLAGNGLLIWFLIFCIKRKPFTI
YILHLAIADFMVLLCSSIMKLVNTFHIYNMTLESYAILFMIFGYNTGLHLLTAISVERCLSVLYPIWYQC
QRPKHQSAVACMLLWALSVLVSGLENFFCILEVKPQFPECRYVYIFSCILTFLVFVPLMIFSNLILFIQV
CCNLKPRQPTKLYVIIMTTVILFLVFAMPMKVLLIIGYYSSSLDDSVWDSLPYLNMLSTINCSINPIVYF
VVGSLRRKRSRKSLKEALQKVFEEKPVVASRENVTQFSLPS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030726
ORF Size 963 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030726.1, NP_109651.1
RefSeq Size 1809 bp
RefSeq ORF 966 bp
Locus ID 80978
UniProt ID Q99MT8
Cytogenetics 17 A1
MW 36.9 kDa
Summary Orphan receptor. May regulate nociceptor function and/or development, including the sensation or modulation of pain (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_030726" in other vectors (4)
SKU Description Size Price
MC211939 Mrgprh (untagged) - Mouse MAS-related GPR, member H (Mrgprh), (10ug) 10 ug
$330.00
MG220655 Mrgprh (tGFP-tagged) - Mouse MAS-related GPR member H (Mrgprh), (10ug) 10 ug
$500.00
MR220655L3 Lenti ORF clone of Mrgprh (Myc-DDK-tagged) - Mouse MAS-related GPR, member H (Mrgprh) 10 ug
$600.00
MR220655L4 Lenti ORF clone of Mrgprh (mGFP-tagged) - Mouse MAS-related GPR, member H (Mrgprh) 10 ug
$600.00

Plasmid Map for MR220655

Download sequence for

Cloning scheme for MR220655

Circular map for MR220655

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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