Il22ra2 (NM_178258) Mouse Tagged ORF Clone
$450.00
2 Weeks*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Il22ra2 |
| Synonyms | CRF2-10; CRF2-s1; CRF2X; Il-22bp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_178258 |
| ORF Size | 690 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_178258.5, NP_839989.2 |
| RefSeq Size | 2468 bp |
| RefSeq ORF | 693 bp |
| Locus ID | 237310 |
| UniProt ID | Q80XF5 |
| Cytogenetics | 10 A3 |
| MW | 27.1 kDa |
| Summary | Receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_178258" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC213036 | Il22ra2 (untagged) - Mouse interleukin 22 receptor, alpha 2 (Il22ra2), (10ug) | 10 ug |
$450.00
|
|
| MG220602 | Il22ra2 (tGFP-tagged) - Mouse interleukin 22 receptor alpha 2 (Il22ra2), (10ug) | 10 ug |
$650.00
|
|
| MR220602L3 | Lenti ORF clone of Il22ra2 (Myc-DDK-tagged) - Mouse interleukin 22 receptor, alpha 2 (Il22ra2) | 10 ug |
$750.00
|
|
| MR220602L4 | Lenti ORF clone of Il22ra2 (mGFP-tagged) - Mouse interleukin 22 receptor, alpha 2 (Il22ra2) | 10 ug |
$750.00
|
|
Citations
Delivery of interleukin-22 binding protein (IL-22BP) gene by cationic micelle for colon cancer gene therapy
Men, Huang, Zhang et al
RSC Adv. (2018) 8 (30), 16537-16548 DOI: 10.1039/c8ra02580k
Men, Huang, Zhang et al
RSC Adv. (2018) 8 (30), 16537-16548 DOI: 10.1039/c8ra02580k
AI Summary: OriGene’s murine IL-22BP cDNA was used as the gene source for amplification and subsequent delivery in the study, enabling evaluation of IL-22BP gene therapy for colon cancer.
Snippet: Murine IL-22BP cDNA (Origene, USA) was amplified (forward: GGG AAA GCT TAT GAT GCC TAA GCA TTG CCT TCT AGG, reverse: GGG ATC TAG ATC ATG GAA TGT GCA CAC ATC TCT CC) and digested with Hind III and XbaI. pVAX1 plasmid was used as the empty
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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