Pitrm1 (NM_145131) Mouse Tagged ORF Clone

SKU MR220510

Pitrm1 (Myc-DDK-tagged) - Mouse pitrilysin metallepetidase 1 (Pitrm1), nuclear gene encoding mitochondrial protein

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Pitrm1
• Synonyms: 2310012C15Rik; AA410010; mKIAA1104; MP-1; MP1; Ntup1; PreP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220510 representing NM_145131; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTGGCGCTTCAGCGGTCGGCGGGGACTCTGCGCTGTACAGCGGCTGAGCTGCGGCAGGGTACACCACA; GAGTATGGAGGGAGAAGAGTGACCAAGCCTGTGAACGAGCTCTACAGTATAAAGTGGGAGAGAAAATCCA; CGGGTTCACTGTAAACCAGGTCACTCCTGTCCCCGAGCTGTTCCTGACAGCCGTGAAGCTCAGCCATGAC; AACACGGGAGCCAGATACCTGCACCTGGCAAGGGAAGACAAGAACAACTTATTCAGTGTGCAGTTCCGCA; CAACCCCAATGGATAGCACTGGGGTCCCACATGTTCTCGAGCATACGGTCCTGTGCGGCTCTCAGAAGTA; CCCGTGCAGAGATCCTTTCTTCAAAATGCTCAACAGGTCACTGTCCACATTTATGAATGCCATGACAGCC; AGCGATTACACGATATATCCGTTTTCCACTCAAAATCCCAAAGATTTTCAGAACCTCCTCTCCGTGTATT; TGGATGCAACTTTCTTCCCCTGCTTGAGGGAACTGGACTTCTGGCAGGAAGGATGGCGTCTGGAGCATGA; GAATCCCCGAGACCCTCAGACGCCCTTGATCTTTAAGGGGGTCGTCTTCAACGAGATGAAAGGGGCATTT; ACAGACAATGAGAGGATATTCTCCCAGCACCTGCAGAACAAGCTGCTTCCTGACCACACCTACTCCGTGG; TTTCTGGAGGGGACCCACTGTGCATCCCGGAGCTCACGTGGGAACAGCTGAAACAGTTCCACGCTACTCA; TTATCACCCAAGCAATGCCAGGTTCTTCACTTATGGCAATTTTCAGCTGGAAGGACACCTGAAACAAATT; CACGAAGAAGCCCTGAGTAAATTCCAGAGATTGGAGCAGAGTACAGCAGTGCCTGCCCAGCCGCACTGGG; ATAAGCCTAGGGAATTCCATATAACATGTGGCCCAGATTCACTAGCTACGGAGACTGCCAAGCAGACAAC; TGTCAGCGTTAGCTTCCTCTTACCGGATATCACTGACACATTTGAAGCCTTCACCTTGAGCCTTCTGTCC; TCCCTCCTGATTGCTGGACCCAACTCCCCCTTCTACAAAGCTTTGATCGAGTCTGGACTCGGCACAGACT; TTTCTCCTGATGTTGGATATAATGGCTATACACGGGAGGCTTACTTCAGTGTCGGGCTCCAAGGGATCGC; AGAGAAAGATGTCAAGACGGTCAGAGAGCTCGTAGACAGGACAATCGAAGAAGTTATAGAGAAAGGATTT; GAAGATGATCGGATTGAAGCTCTGCTTCATAAAATCGAAATTCAAACGAAGCATCAGTCAGCCAGCTTTG; GCCTGACCCTGACGTCATATATAGCTTCTTGCTGGAACCATGATGGGGACCCTGTGGAGCTCCTGCAGAT; TGGAAGTCAGCTGACTAGATTTAGGAAGTGCCTTAAGGAAAATCCAAAATTTTTACAAGAAAAAGTAGAA; CAATATTTTAAGAACAATCAGCACAAGCTGACTTTATCCATGAAGCCAGACGACAAGTATTATGAAAAGC; AAACTCAGATGGAGACAGAAAAGCTGGAGCAAAAGGTGAATTCTCTCTCCCCGGCGGACAAGCAGCAGAT; CTACGAGAAAGGTTTAGAACTACAGACGCAGCAAAGTAAACATCAAGACGCCTCCTGCCTCCCAGCATTG; AAAGTCTCGGACATTGAGCCCTCCATGCCTTTCACCAAGCTTGACATCGGCCTTGCAGCTGGAGACATCC; CTGTGCAGTACTGCCCACAGCCCACCAACGGCATGGTGTATTTCCGAGCCTTTTCCAGTTTAAACACGCT; GCCGGAGGACCTGAGGCCCATTGTGCCTCTCTTTTGCAGCGTGCTGACCAAGCTGGGTTGTGGCATCCTT; AACTACAGAGAGCAAGCCCAACAGATTGAGCTCAAGACAGGAGGCATGAGTGTCACGCCCCATGTGCTCC; CTGACGACTCACAGCTGGATACCTACGAGCAGGGTGTGTTATTTTCATCTCTCTGCCTGGAGCGGAACCT; GCCAGACATGATGCATCTGTGGAGCGAAATATTTAACAATCCATGCTTTGAAGAAGAAGAACACTTCAAA; GTGTTGGTGAAGATGACCGCTCAGGAGCTCTCCAATGGAATTTCAGACTCGGGGCATCTCTATGCAGCCC; TCAGAGCAAGCAAGACACTGACACCTTCAGGGGACTTGCAGGAGACCTTCAGTGGGATGGATCAGGTGAA; GGTGATGAAAAGAATTGCAGAGATGACAGACATCAAGCCCATCCTGAGAAAACTGCCCCGGATCAAGAAG; TATCTACTAAACTGTGACAACATGAGATGCTCAGTGAATGCCACCCCTCAGCAGATGCCTCAGGCAGAAA; AAGAGGTGGAAAACTTCCTTAGAAATGTTGGCCGAAGCAAAAAGGAACGGAAGCCTGTCCGCCCGCATAT; TGTCGAGAAACCCACACCCAGTGGCCCCAGTGGAGCTGCACATGTCAGTGGGTCCCAGATCGTCAGAAAA; TTGGTGACAGACCCCACCTTCAAACCCTGCCAGATGAAGACACATTTTGTGCTGCCCTTCCCTGTGAATT; ACATTGGCGAGTGTGTCAGGACTGTCCCGTATGCTGATCCAGACCATGCCAGCCTTAAGATCCTTGCCCG; TCTAATGACAGCTAAATTCTTGCATACGGAAATTCGAGAGAAGGGGGGTGCTTATGGTGGCGGTGCTAAA; CTCACCCACAGTGGGATTTTCACGCTTTACTCTTACAGGGATCCCAATTCCATAGAAACACTCCAGTCTT; TTGGGAAAGCTGTAGACTGGGCTAAGTCTGGAAAGTTCACACAGCAGGACATTGATGAAGCCAAGCTGTC; TGTTTTCTCTACTGTGGATTCTCCTGTTGCTCCATCCGATAAAGGAATGGACCACTTCTTGTATGGCCTC; TCCGATGAGATGAAGCAGGCATACCGAGAACAGCTCTTTGCTGTCAACCACGACAAACTGACCTCTGTGA; GCCATAAATACCTTGGCATCGGGAAGAGCACACACGGCCTGGCTATCCTCGGACCAGAGAACTCAAAAAT; TGCCAAAGACCCATCATGGATCATAAAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220510 representing NM_145131; Red=Cloning site Green=Tags(s); ; MWRFSGRRGLCAVQRLSCGRVHHRVWREKSDQACERALQYKVGEKIHGFTVNQVTPVPELFLTAVKLSHD; NTGARYLHLAREDKNNLFSVQFRTTPMDSTGVPHVLEHTVLCGSQKYPCRDPFFKMLNRSLSTFMNAMTA; SDYTIYPFSTQNPKDFQNLLSVYLDATFFPCLRELDFWQEGWRLEHENPRDPQTPLIFKGVVFNEMKGAF; TDNERIFSQHLQNKLLPDHTYSVVSGGDPLCIPELTWEQLKQFHATHYHPSNARFFTYGNFQLEGHLKQI; HEEALSKFQRLEQSTAVPAQPHWDKPREFHITCGPDSLATETAKQTTVSVSFLLPDITDTFEAFTLSLLS; SLLIAGPNSPFYKALIESGLGTDFSPDVGYNGYTREAYFSVGLQGIAEKDVKTVRELVDRTIEEVIEKGF; EDDRIEALLHKIEIQTKHQSASFGLTLTSYIASCWNHDGDPVELLQIGSQLTRFRKCLKENPKFLQEKVE; QYFKNNQHKLTLSMKPDDKYYEKQTQMETEKLEQKVNSLSPADKQQIYEKGLELQTQQSKHQDASCLPAL; KVSDIEPSMPFTKLDIGLAAGDIPVQYCPQPTNGMVYFRAFSSLNTLPEDLRPIVPLFCSVLTKLGCGIL; NYREQAQQIELKTGGMSVTPHVLPDDSQLDTYEQGVLFSSLCLERNLPDMMHLWSEIFNNPCFEEEEHFK; VLVKMTAQELSNGISDSGHLYAALRASKTLTPSGDLQETFSGMDQVKVMKRIAEMTDIKPILRKLPRIKK; YLLNCDNMRCSVNATPQQMPQAEKEVENFLRNVGRSKKERKPVRPHIVEKPTPSGPSGAAHVSGSQIVRK; LVTDPTFKPCQMKTHFVLPFPVNYIGECVRTVPYADPDHASLKILARLMTAKFLHTEIREKGGAYGGGAK; LTHSGIFTLYSYRDPNSIETLQSFGKAVDWAKSGKFTQQDIDEAKLSVFSTVDSPVAPSDKGMDHFLYGL; SDEMKQAYREQLFAVNHDKLTSVSHKYLGIGKSTHGLAILGPENSKIAKDPSWIIK; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145131
• ORF Size: 3108 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145131.1, NP_660113.1
• RefSeq Size: 3547 bp
• RefSeq ORF: 3111 bp
• Locus ID: 69617
• UniProt ID: Q8K411
• Cytogenetics: 13 A1
• MW: 117.4 kDa
• Summary: Metalloendopeptidase of the mitochondrial matrix that functions in peptide cleavage and degradation rather than in protein processing. Has an ATP-independent activity. Specifically cleaves peptides in the range of 5 to 65 residues. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. Degrades the transit peptides of mitochondrial proteins after their cleavage. Also degrades other unstructured peptides. It is also able to degrade amyloid-beta protein 40, one of the peptides produced by APP processing, when it accumulates in mitochondrion. It is a highly efficient protease, at least toward amyloid-beta protein 40. Cleaves that peptide at a specific position and is probably not processive, releasing digested peptides intermediates that can be further cleaved subsequently.[UniProtKB/Swiss-Prot Function]