OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Gpr75 (NM_175490) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR220478
Gpr75 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 75 (Gpr75)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $504.00
5 Days*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Gpr75 or NM_175490"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpr75
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220478 representing NM_175490
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATT
CGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC

ATGAACACAAGTGCCCCGCTTCAGAATGTCCCCAATGCCACCTTGCTAAACATGCCTCCC
CTGCACGGGGGAAATAGCACTTCTCTCCAGGAAGGTCTTCGAGATTTTATCCACACAGCC
ACCTTGGTGACCTGCACTTTTCTGCTTGCCATCATCTTCTGTCTAGGCTCTTATGGAAAT
TTTATTGTCTTCTTGTCTTTCTTTGACCCATCCTTCAGGAAGTTCAGAACCAACTTTGAT
TTCATGATCTTGAACCTGTCTTTCTGTGATCTGTTCATCTGTGGGGTCACAGCCCCCATG
TTCACCTTCGTGCTGTTCTTCAGCTCAGCCAGTAGCATCCCAGATAGCTTCTGCTTCACC
TTCCACCTTACCAGCTCAGGCTTCGTCATCATGTCCCTCAAGATGGTGGCTGTGATTGCT
CTGCACCGGCTCCGGATGGTGATGGGGAAGCAGCCTAATTGTACAGCCTCCTTTTCCTGC
ATCTTGCTCCTTACCCTTCTTCTCTGGGCGACCAGCTTTACACTTGCCACCTTGGCTACA
CTGAGAACCAATAAGTCCCACCTGTGTCTCCCCATGTCCAGTCTTATGGATGGGGAAGGG
AAAGCCATTCTGTCTCTGTATGTTGTTGACTTTACCTTCTGTGTTGCTGTGGTGTCTGTC
TCTTATATTATGATTGCTCAAACCCTTCGGAAAAATGCTCAAGTAAAAAAGTGCCCCCCG
GTGATCACAGTTGATGCTTCCAGACCACAGCCATTCATGGGGGCCTCTGTGAAGGGAAAT
GGAGATCCCATCCAGTGCACCATGCCAGCTCTGTATAGGAACCAGAATTATAACAAACTG
CAGCACAGTCAAACTCATGGATACACTAAGAATATCAACCAGATGCCAATCCCCTCAGCC
AGTCGACTCCAGCTGGTATCAGCCATCAACTTCTCTACTGCCAAGGATTCCAAAGCCGTG
GTCACCTGTGTGGTCATCGTGTTGTCAGTCCTGGTGTGCTGTCTTCCTCTTGGGATTTCC
CTGGTGCAAATGGTTCTGTCTGACAATGGCAGTTTTATCCTTTACCAGTTTGAACTGTTT
GGATTTACTCTGATATTTTTCAAGTCAGGATTAAATCCTTTTATATATTCTCGGAACAGT
GCTGGGCTGAGAAGGAAAGTACTCTGGTGCCTGAGATACACTGGCCTGGGCTTTCTCTGC
TGCAAACAGAAAACTCGACTTCGGGCCATGGGAAAAGGGAACCTTGAAATCAATAGAAAC
AAATCTTCTCATCATGAGACAAACTCTGCCTACATGCTGTCTCCAAAACCACAGAGAAAA
TTTGTGGACCAGGCTTGTGGCCCAAGTCACTCAAAGGAAAGTGCAGCGAGTCCCAAAGTT
TCTGCTGGACATCAACCCTGTGGTCAAAGCAGTTCCACACCCATCAACACTAGGATTGAA
CCTTACTATAGCATCTATAACAGCAGCCCTTCCCAGCAGGAGAGCGGTCCGGCAAACTTG
CCTCCAGTGAACTCTTTTGGGTTTGCCAGTTCCTACATCGCCATGCACTATTACACCACC
AATGATTTGATGCAGGAATATGACAGCACGTCAGCAAAACAAATTCCTATCCCCTCTGTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGAT
GACGACGATAAGGTTTAAA
Protein Sequence
>MR220478 protein sequence
Blue=ORF Red=Cloning site Green=Tag(s)

MNTSAPLQNVPNATLLNMPPLHGGNSTSLQEGLRDFIHTATLVTCTFLLAIIFCLGSYGN
FIVFLSFFDPSFRKFRTNFDFMILNLSFCDLFICGVTAPMFTFVLFFSSASSIPDSFCFT
FHLTSSGFVIMSLKMVAVIALHRLRMVMGKQPNCTASFSCILLLTLLLWATSFTLATLAT
LRTNKSHLCLPMSSLMDGEGKAILSLYVVDFTFCVAVVSVSYIMIAQTLRKNAQVKKCPP
VITVDASRPQPFMGASVKGNGDPIQCTMPALYRNQNYNKLQHSQTHGYTKNINQMPIPSA
SRLQLVSAINFSTAKDSKAVVTCVVIVLSVLVCCLPLGISLVQMVLSDNGSFILYQFELF
GFTLIFFKSGLNPFIYSRNSAGLRRKVLWCLRYTGLGFLCCKQKTRLRAMGKGNLEINRN
KSSHHETNSAYMLSPKPQRKFVDQACGPSHSKESAASPKVSAGHQPCGQSSSTPINTRIE
PYYSIYNSSPSQQESGPANLPPVNSFGFASSYIAMHYYTTNDLMQEYDSTSAKQIPIPSV


TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175490
ORF Size 1620 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175490.3, NM_175490.4, NP_780699.2
RefSeq Size 1623 bp
RefSeq ORF 1623 bp
Locus ID 237716
UniProt ID Q6X632
Cytogenetics 11 A4
MW 60 kDa
Summary G protein-coupled receptor that is activated by the chemokine CCL5/RANTES. Probably coupled to heterotrimeric Gq proteins, it stimulates inositol trisphosphate production and calcium mobilization upon activation. Together with CCL5/RANTES, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. CCL5/RANTES may also regulate insulin secretion by pancreatic islet cells through activation of this receptor.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_175490" in other vectors (4)
SKU Description Size Price
MC218627 Gpr75 (untagged) - Mouse G protein-coupled receptor 75 (Gpr75), (10ug) 10 ug
$554.00
MG220478 Gpr75 (tGFP-tagged) - Mouse G protein-coupled receptor 75 (Gpr75), (10ug) 10 ug
$489.00 $704.00
MR220478L3 Lenti ORF clone of Gpr75 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 75 (Gpr75) 10 ug
$804.00
MR220478L4 Lenti ORF clone of Gpr75 (mGFP-tagged) - Mouse G protein-coupled receptor 75 (Gpr75) 10 ug
$804.00

Plasmid Map for MR220478

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220478
Circular map for MR220478

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products