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Defa21 (NM_183253) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220465
Defa21 (Myc-DDK-tagged) - Mouse defensin, alpha, 21 (Defa21)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Defa21 or NM_183253"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Defa21
Synonyms 2010016B13Rik; Defcr21
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220465 representing NM_183253
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGACACTTGTCCTCCTCTCTGCCCTCATCCTGCTGGCCTACCAGGTCCAGACTGATCCTATCCAAA
ACACAGATGAAGAGACTAATACTGAGGAGCAGCCAGGGGAAGATGACCAGGCTGTGTCTGTCTCCTTTGG
AGGCCAAGAAGGATCTGCTCTTCATGAAAAATTGTCGAGAGATCTGATCTGCCTTTGTAGAAATCGTCGC
TGCAATAGAGGAGAACTATTTTATGGGACCTGCGCAGGACCTTTTTTGCGCTGCTGCCGCCGCCGCCGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220465 representing NM_183253
Red=Cloning site Green=Tags(s)

MKTLVLLSALILLAYQVQTDPIQNTDEETNTEEQPGEDDQAVSVSFGGQEGSALHEKLSRDLICLCRNRR
CNRGELFYGTCAGPFLRCCRRRR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_183253
ORF Size 279 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_183253.3, NP_899076.1
RefSeq Size 419 bp
RefSeq ORF 282 bp
Locus ID 66298
UniProt ID Q8C1P2
Cytogenetics 8 A2
MW 11 kDa
Summary May have microbicidal activities.UniProtKB/Swiss-Prot Function
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"NM_183253" in other vectors (4)
SKU Description Size Price
MC210322 Defa21 (untagged) - Mouse defensin, alpha, 21 (Defa21), (10ug) 10 ug
$165.00
MG220465 Defa21 (tGFP-tagged) - Mouse defensin alpha 21 (Defa21), (10ug) 10 ug
$350.00
MR220465L3 Lenti ORF clone of Defa21 (Myc-DDK-tagged) - Mouse defensin, alpha, 21 (Defa21) 10 ug
$450.00
MR220465L4 Lenti ORF clone of Defa21 (mGFP-tagged) - Mouse defensin, alpha, 21 (Defa21) 10 ug
$450.00

Plasmid Map for MR220465

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220465
Circular map for MR220465

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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