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Ccdc114 (NM_001033243) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220438
Ccdc114 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 114 (Ccdc114)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $612.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ccdc114
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220438 representing NM_001033243
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGAGTTGGAAAGGCGGGCATACAGCAAGGAAGTTCATCAGCGCCTCAGGAAGCAAGTCGAGGAGA
TTCGGCAGCTGGAGATGCTTCGAGCCAAGCTCCAGACGCAGATTAACGTAGCGCAGAGCCAGGTCAAGCG
ACTGGGAGACAAGAAGCATTTGGCGGAAATGGAATGTCTTCTCAAGTCTCGGGCCCAGGTGCAGGTGGAA
ATCGAGGCGCTGCAAGAGCAGAACCGGGCCCTGGACAAGCAGATCCAGGACTGGGAGACCCATGTCCTCA
CGCAGAGTAAGGAGGCCTCGGCTCCCGATCTCATCATGTACCAGAAGATGAAAATCCAGAGAAGGATCAG
GATCCTTGAAGACCAGCTGGACAGGGTCACCTGTCATTTTGACATCCATCTGGTGAGGAATGCAGCCCTG
CGGGAGGAGCTGGAGCTGCTGCGCATCGAGAGGGGCCGCTACCTGAATATGGACCGCAAGCTGAAGAAGG
AGATCCACCTACTCCGGGAGATGGTTGGAGCTCTCAGCACATCCTCTACTTCTGCTTATACTGCCAGGGA
AGAGGCCAAGACCAAGATGGGCATGCTTCAGGAGCGCGCAGAGAAGGAGCTGGCTCAGAGTGATACGGAG
GCACAGATCTTGCTGCGACAGATATCGCATCTGGAGCAGCTGCATCGATTCCTTAAGCTCAAGAACGATG
AACGACAACCGGATCCCCGTGTGGTGCAAAAGGCAGAACAGCGAGACTGGGAAGTGTCAGAAGGACTCCG
AAAGACCTCCCAAGAGAAGCTAGTGCTGCGTTATGAGGACACTCTGGGCAAACTGGCCCAGCTGACCGGC
GAGAGCGACCCTGACCTTTTGGTGGAGAAATACCTGGAGTTGGAGGAACGCAACTTTGCTGAGTTTAACT
TCATCAATGAGCAGAACTCCGAGATACACCACCTTCAGGAGGAGATCAAAGAGATGCAGGAAGCCTTAGT
CAGTGAGCATGCCAGTCAGGATAAACAGCGCATGCAGCAAGAGCAGCAGTGCAAAATGTTGCAGCAGGAT
GTGGACAAGATGTGTTCTGAGTCAGAGCAGCTGGAGGGTCGCTTCCAGGTGCTCCGAGGTCAGCTGGAAA
AAATCAAGACTGATATCCAGGTGCTCTTCGACAAGGCTAAGTGTGACAGCAGTGTCATCAAGGATCTCCT
TGGGGTCAAGACCTACATGCGGGACAGGGACATAGGCCTCTTCCTGAGTACCATTGAGAGGCGGCTGGTG
CAGCTCCTCACTGTGCAGGCCTTCCTCCAGGTCCAGAACCTTGCCCCTTTGGCTGATGCTGCACTTCTAG
CACTGGGCCAGAGCCTACAGGAACCCTCCAAGAAGACGACTCCACTCAAGCCTCCTGATACCATGGAGGA
CTCCTCAGGGGCTGTAATCAAAGAGGATTACCCTATGAGCAAGGAGGAGTTGCTAAGCCAAGTCATGAAG
TCGCTGCAGCTCCAGGACGAGGAGGAGTCCGCCAAGAAGCTGGACAGCAGCCCGAGCTTGACTCTCTCCA
GTCCACAGATCTCCCTGGTCACTGTCCCCAAGCATTCCAAGAAGACCAGTGTAGTCCCCGAATCCATCCT
GAGCCACAAGACGAACCGAGGCCGTGGCACTGGCTCCGTGAGCCATGTCACCTTTGGTGACTCTGCCTCT
GCTGCAGGCCCTGTGGCCATGGCTTCTGCCAGTGCCAGTGGAGCACCTGTGTCCAGTCGGAGCTCCCAGG
GGGGCCGTGGGGGCTTCAAACCCACGAGCTCCAGCAGCTACCTGGGGTCTACAGGCTACCTAGAGACCAG
CAGAGGCCGCGAGAGCACAGCAGGCGGCGTTCACAGCCAGAGCATGGGATCTGAGTTGAGTAGAGGCCTC
AGTTCCAGCAGTGGCCATGCATCCAGTCCTGCCCCTCCCTCCAGGCCCAGCTCCTCTACTAGCAAGGACT
CCAGGGGCTACAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220438 representing NM_001033243
Red=Cloning site Green=Tags(s)

MMELERRAYSKEVHQRLRKQVEEIRQLEMLRAKLQTQINVAQSQVKRLGDKKHLAEMECLLKSRAQVQVE
IEALQEQNRALDKQIQDWETHVLTQSKEASAPDLIMYQKMKIQRRIRILEDQLDRVTCHFDIHLVRNAAL
REELELLRIERGRYLNMDRKLKKEIHLLREMVGALSTSSTSAYTAREEAKTKMGMLQERAEKELAQSDTE
AQILLRQISHLEQLHRFLKLKNDERQPDPRVVQKAEQRDWEVSEGLRKTSQEKLVLRYEDTLGKLAQLTG
ESDPDLLVEKYLELEERNFAEFNFINEQNSEIHHLQEEIKEMQEALVSEHASQDKQRMQQEQQCKMLQQD
VDKMCSESEQLEGRFQVLRGQLEKIKTDIQVLFDKAKCDSSVIKDLLGVKTYMRDRDIGLFLSTIERRLV
QLLTVQAFLQVQNLAPLADAALLALGQSLQEPSKKTTPLKPPDTMEDSSGAVIKEDYPMSKEELLSQVMK
SLQLQDEEESAKKLDSSPSLTLSSPQISLVTVPKHSKKTSVVPESILSHKTNRGRGTGSVSHVTFGDSAS
AAGPVAMASASASGAPVSSRSSQGGRGGFKPTSSSSYLGSTGYLETSRGRESTAGGVHSQSMGSELSRGL
SSSSGHASSPAPPSRPSSSTSKDSRGYN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033243
ORF Size 1974 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033243.2, NP_001028415.1
RefSeq Size 2453 bp
RefSeq ORF 1977 bp
Locus ID 211535
UniProt ID Q3UX62
Cytogenetics 7 B3
MW 74.1 kDa
Summary Probable component of the outer dynein arm complex required along the entire axoneme for tethering of outer dynein arms.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001033243" in other vectors (4)
SKU Description Size Price
MC220199 Ccdc114 (untagged) - Mouse coiled-coil domain containing 114 (Ccdc114), (10ug) 10 ug
$675.00
MG220438 Ccdc114 (tGFP-tagged) - Mouse coiled-coil domain containing 114 (Ccdc114), (10ug) 10 ug
$812.00
MR220438L3 Lenti ORF clone of Ccdc114 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 114 (Ccdc114) 10 ug
$912.00
MR220438L4 Lenti ORF clone of Ccdc114 (mGFP-tagged) - Mouse coiled-coil domain containing 114 (Ccdc114) 10 ug
$912.00

Plasmid Map for MR220438

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220438
Circular map for MR220438

file-alt Citations

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