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Gpr87 (NM_032399) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220429
Gpr87 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 87 (Gpr87)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpr87
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220429 representing NM_032399
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGTCCCTAATGTGAATGTTTCTACTTTTGCAGGTAATGAGCTGTACAGCCAAGCAAGTCACACTG
CAAACAGCACAAGTGAGGGACATGGGAAGAACTCTACCCTGCACAACAAATTTGACACCATCATCCTGCC
AGTGCTTTACCTAGTTATCTTTGTGGCAAGCATCCTGCTGAACGGTCTGGCCGTGTGGATCTTCTTCCAC
ATTCGGAATAAAACCAGCTTCATATTTTATCTCAAAAATATAGTGGTAGCTGACCTCATCATGACCCTGA
CATTCCCATTCCGAATAGTCCGTGATGCAGGATTCGGACCTTGGTACTTCGAGTTTATCCTCTGCAGATA
CACCTCAGTTTTGTTCTATGCAAACATGTATACATCTATCGTGTTTCTTGGGCTGATCAGTGTTGATCGG
TATCTAAAGGTGGTAAAGCCCTTTGGTGACTCTCGCATGTACAGCATAACCTTCACCAAAGTTTTATCAG
TTTGTGTTTGGGTGATCATGGCTATTCTGTCCTTGCCAAACATCATACTGACTAATGGGCAGCCAACTAA
GGAAAATATTCATGACTGCATGAAACTCAAAAGTCCGCTGGGAGCCAAGTGGCATATGGCTGTCACCTAT
GTGGACAGCTGTTTGTTTGTGGCCGTGCTGGTGATCCTGATTGGATGCTACATAGCCATCTCCAGATACA
TCCACAAATCCAGCAGGCAATTCATAAGCCAATCGAGCCGGAAGCGAAAGCACAACCAGAGCATCCGCGT
GGTCGTGGCTGTGTTTTTTACCTGCTTCCTCCCGTATCACTTGTGCAGAATCCCCTTTACCTTCAGTAAC
TTAGACAGGCTTCTGGATGAATCAGCACATAAAATCCTCTACTATTGCAAAGAAATGACACTTTTCTTGT
CTGCGTGCAACGTGTGCCTGGATCCGATAATTTATTTTTTCATGTGTAAGTCATTTTCAAGAAGGTTATT
CAAGAAATCAAACATAAGAACCAGGAGCGAAAGCATCAGGTCGCTGCAAAGCGTCCGAAGATCAGAAGTG
CGCATCTATTATGACTACACTGATGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220429 representing NM_032399
Red=Cloning site Green=Tags(s)

MAVPNVNVSTFAGNELYSQASHTANSTSEGHGKNSTLHNKFDTIILPVLYLVIFVASILLNGLAVWIFFH
IRNKTSFIFYLKNIVVADLIMTLTFPFRIVRDAGFGPWYFEFILCRYTSVLFYANMYTSIVFLGLISVDR
YLKVVKPFGDSRMYSITFTKVLSVCVWVIMAILSLPNIILTNGQPTKENIHDCMKLKSPLGAKWHMAVTY
VDSCLFVAVLVILIGCYIAISRYIHKSSRQFISQSSRKRKHNQSIRVVVAVFFTCFLPYHLCRIPFTFSN
LDRLLDESAHKILYYCKEMTLFLSACNVCLDPIIYFFMCKSFSRRLFKKSNIRTRSESIRSLQSVRRSEV
RIYYDYTDV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032399
ORF Size 1077 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032399.1, NP_115775.1
RefSeq Size 1163 bp
RefSeq ORF 1080 bp
Locus ID 84111
Cytogenetics 3 D
MW 41.9 kDa
Summary Receptor for lysophosphatidic acid (LPA). Necessary for p53/TP53-dependent survival in response to DNA damage (By similarity).UniProtKB/Swiss-Prot Function
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"NM_032399" in other vectors (4)
SKU Description Size Price
MC211972 Gpr87 (untagged) - Mouse G protein-coupled receptor 87 (Gpr87), (10ug) 10 ug
$457.00
MG220429 Gpr87 (tGFP-tagged) - Mouse G protein-coupled receptor 87 (Gpr87), (10ug) 10 ug
$657.00
MR220429L3 Lenti ORF clone of Gpr87 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 87 (Gpr87) 10 ug
$757.00
MR220429L4 Lenti ORF clone of Gpr87 (mGFP-tagged) - Mouse G protein-coupled receptor 87 (Gpr87) 10 ug
$757.00

Plasmid Map for MR220429

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220429
Circular map for MR220429

file-alt Citations

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