Raet1b (NM_009017) Mouse Tagged ORF Clone
$289.00
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Raet1b |
| Synonyms | RAE-1beta |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_009017 |
| ORF Size | 759 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_009017.1, NP_033043.1 |
| RefSeq Size | 1242 bp |
| RefSeq ORF | 762 bp |
| Locus ID | 19369 |
| UniProt ID | O08603 |
| MW | 28.6 kDa |
| Summary | Acts as a ligand for KLRK1.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_009017" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC209288 | Raet1b (untagged) - Mouse retinoic acid early transcript beta (Raet1b), (10ug) | 10 ug |
$330.00
|
|
| MG220341 | Raet1b (tGFP-tagged) - Mouse retinoic acid early transcript beta (Raet1b), (10ug) | 10 ug |
$500.00
|
|
| MR220341L3 | Lenti ORF clone of Raet1b (Myc-DDK-tagged) - Mouse retinoic acid early transcript beta (Raet1b) | 10 ug |
$600.00
|
|
| MR220341L4 | Lenti ORF clone of Raet1b (mGFP-tagged) - Mouse retinoic acid early transcript beta (Raet1b) | 10 ug |
$600.00
|
|
Citations
Tumor-induced natural killer cell dysfunction is a rapid and reversible process uncoupled from the expression of immune checkpoints
Pouxvielh, Marotel, Drouillard et al
Sci Adv (2024) 10 (35), eadn0164 DOI: 10.1126/sciadv.adn0164
Pouxvielh, Marotel, Drouillard et al
Sci Adv (2024) 10 (35), eadn0164 DOI: 10.1126/sciadv.adn0164
Cited by 21
AI Summary: In this study, the Rae1-β gene was obtained from the OriGene MR220341 plasmid to generate lentiviral constructs for transducing cells, enabling investigation of NK cell dysfunction mechanisms in tumor models.
Snippet: n (GFP) expression 24 hours after transfection. Cells were sorted for GFP expression 48 hours after transfection with the FACSAria II instrument. Rae1-β gene was subcloned from the MR220341 plasmid (Origene) into the pRRLSIN-MND-IRES2-ZsGreen-WPRE lentiviral vector (p297, Vect’UB vectorology platform, Bordeaux). After amplification, RMA or MHC-I–deficient RMA cells were transduced with the em
Combinatorial Expression of NK Cell Receptors Governs Cell Subset Reactivity and Effector Functions but Not Tumor Specificity
Rocca, Pouxvielh, Marotel et al
J Immunol (2022) 208 (7), 1802-1812 DOI: 10.4049/jimmunol.2100874
Rocca, Pouxvielh, Marotel et al
J Immunol (2022) 208 (7), 1802-1812 DOI: 10.4049/jimmunol.2100874
Cited by 6
AI Summary: In this study, the Rae1β gene obtained from the OriGene plasmid MR220341 was subcloned and used to generate lentiviral vectors for functional analysis of NK cell responses.
Snippet: 48 h after transfection with an FACSAria II (BD Biosciences). The Rae1β gene was subcloned from Origene plasmid MR220341 into the pRRLSIN-MND-IRES2-ZsGreen-WPRE lentiviral vector (p297). After amplification,
Combinatorial Expression of NK Cell Receptors Governs Cell Subset Reactivity and Effector Functions But Not Tumor Specificity
Walzer, Rocca, Pouxvielh et al
SSRN Journal (2021)
Walzer, Rocca, Pouxvielh et al
SSRN Journal (2021)
Snippet: Electronic copy available at: https://ssrn.com/abstract=3872045 Page 15. from Origene plasmid MR220341 into the pRRLSIN-MND-IRES2-ZsGreen-WPRE lentiviral vector (p297). After amplification, MHC Class I deficient RMA cells were transduced with the
Showing 1-3 of 3 papers.
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