Npas4 (NM_153553) Mouse Tagged ORF Clone

SKU MR220190

Npas4 (Myc-DDK-tagged) - Mouse neuronal PAS domain protein 4 (Npas4)

Product Data

• Target Symbol: Npas4
• Synonyms: LE-PAS; Nxf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220190 representing NM_153553; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTACCGATCCACCAAGGGCGCCTCCAAGGCGCGCCGCGACCAGATCAACGCCGAGATTCGGAACCTCA; AGGAGCTGCTGCCGTTGGCTGAAGCGGACAAGGTCCGGCTGTCCTACCTGCACATCATGAGTCTTGCCTG; CATCTACACTCGCAAGGGTGTCTTCTTTGCTGGAGGCACTCCTTTGGCTGGCCCCACCGGGCTTCTCTCT; GCTCAAGAGCTTGAAGACATTGTGGCAGCACTACCTGGATTTCTCCTTGTATTCACAGCTGAGGGGAAGT; TGCTATACCTGTCGGAGAGTGTGAGCGAGCATCTGGGCCACTCTATGGTGGACCTGGTTGCCCAGGGCGA; CAGTATCTACGATATCATTGACCCTGCTGACCATCTCACTGTGCGCCAGCAGCTCACCATGCCCTCTGCT; CTGGATGCTGATCGCCTTTTCCGTTGTCGATTCAACACCTCCAAGTCCCTCCGGCGCCAGAGTTCAGGAA; ACAAACTGGTGCTTATTCGAGGTCGATTCCATGCTCACCCACCTGGGGCCTACTGGGCAGGAAACCCTGT; GTTCACCGCTTTCTGCGCCCCACTGGAGCCAAGACCCCGCCCTGGCCCCGGCCCTGGCCCTGGCCCTGGT; CCTGCTTCTCTCTTCCTGGCCATGTTCCAGAGCCGGCATGCTAAGGACCTAGCCCTACTGGACGTTTCTG; AAAGTGTCCTAATCTACCTGGGCTTTGAGCGCAGCGAACTGCTCTGTAAATCATGGTATGGACTGCTACA; CCCCGAGGACCTGGCCCAAGCTTCTTCTCAACACTACCGCCTGTTGGCTGAAAGTGGAGATATTCAGGCT; GAAATGGTGGTGAGACTTCAAGCCAAGCATGGAGGCTGGACATGGATTTACTGCATGCTATACTCAGAAG; GTCCAGAAGGCCCTATTACTGCCAATAACTACCCTATCAGTGACACGGAAGCCTGGAGCCTCCGCCAGCA; GCTAAACTCTGAAGACACCCAGGCAGCCTATGTCCTAGGAACCCCAGCTGTGCTACCCTCATTCTCTGAG; AATGTCTTCTCCCAGGAGCAATGCTCTAATCCACTCTTTACACCATCCCTGGGGACTCCTAGAAGTGCCA; GCTTCCCCAGGGCTCCTGAACTAGGTGTGATCTCAACACCAGAAGAGCTTCCCCAACCCTCCAAAGAGCT; GGACTTCAGTTACCTGCCATTCCCTGCTAGGCCTGAGCCTTCCCTCCAAGCAGACCTGAGCAAGGATTTG; GTGTGTACTCCACCTTACACACCCCACCAGCCAGGAGGCTGTGCCTTCCTCTTCAGCCTCCATGAACCCT; TCCAGACTCACTTGCCCCCTCCGTCCAGCTCTCTCCAAGAACAGCTGACACCAAGTACAGTGACTTTCTC; TGAACAGTTGACACCCAGCAGTGCTACCTTCCCAGACCCACTAACCAGTTCACTACAAGGACAGTTGACA; GAAAGCTCAGCCAGAAGCTTTGAAGACCAGTTGACTCCATGCACCTCTTCCTTCCCTGACCAGCTACTTC; CCAGCACTGCCACATTCCCAGAGCCTCTGGGCAGCCCCGCCCATGAGCAGCTGACTCCTCCCAGCACAGC; ATTCCAGGCTCATCTGAACAGCCCCAGCCAAACCTTCCCAGAGCAACTGAGCCCCAATCCTACCAAGACT; TACTTCGCCCAGGAGGGATGCAGTTTTCTCTATGAGAAGTTGCCCCCAAGTCCTAGCAGCCCTGGTAATG; GGGACTGTACACTCCTGGCCCTAGCTCAGCTCCGGGGCCCCCTCTCTGTGGATGTCCCCCTGGTGCCCGA; AGGCCTGCTCACACCTGAGGCCTCTCCAGTCAAGCAAAGTTTCTTCCACTACACAGAGAAAGAGCAAAAT; GAGATAGATCGTCTCATTCAGCAGATCAGCCAGTTGGCTCAGGGCGTGGACAGGCCCTTCTCAGCTGAGG; CTGGCACTGGGGGGCTGGAGCCACTTGGAGGGCTGGAGCCCCTGAACCCTAACCTGTCCCTGTCAGGGGC; TGGACCCCCTGTGCTTAGCCTGGATCTTAAACCCTGGAAATGCCAGGAGCTGGACTTCCTGGTTGACCCT; GATAATTTATTCCTGGAAGAGACGCCAGTGGAAGACATCTTCATGGATCTTTCTACTCCAGACCCCAATG; GGGAATGGGGTTCAGGGGATCCTGAGGCAGAGGTCCCAGGAGGGACCCTGTCACCTTGCAACAACCTGTC; CCCAGAAGATCACAGCTTCCTGGAGGACTTGGCCACCTATGAAACCGCCTTTGAGACAGGTGTCTCAACA; TTCCCCTACGAAGGGTTTGCTGATGAGTTGCATCAACTCCAGAGCCAAGTTCAAGACAGCTTCCATGAAG; ATGGAAGTGGAGGGGAACCAACGTTT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220190 representing NM_153553; Red=Cloning site Green=Tags(s); ; MYRSTKGASKARRDQINAEIRNLKELLPLAEADKVRLSYLHIMSLACIYTRKGVFFAGGTPLAGPTGLLS; AQELEDIVAALPGFLLVFTAEGKLLYLSESVSEHLGHSMVDLVAQGDSIYDIIDPADHLTVRQQLTMPSA; LDADRLFRCRFNTSKSLRRQSSGNKLVLIRGRFHAHPPGAYWAGNPVFTAFCAPLEPRPRPGPGPGPGPG; PASLFLAMFQSRHAKDLALLDVSESVLIYLGFERSELLCKSWYGLLHPEDLAQASSQHYRLLAESGDIQA; EMVVRLQAKHGGWTWIYCMLYSEGPEGPITANNYPISDTEAWSLRQQLNSEDTQAAYVLGTPAVLPSFSE; NVFSQEQCSNPLFTPSLGTPRSASFPRAPELGVISTPEELPQPSKELDFSYLPFPARPEPSLQADLSKDL; VCTPPYTPHQPGGCAFLFSLHEPFQTHLPPPSSSLQEQLTPSTVTFSEQLTPSSATFPDPLTSSLQGQLT; ESSARSFEDQLTPCTSSFPDQLLPSTATFPEPLGSPAHEQLTPPSTAFQAHLNSPSQTFPEQLSPNPTKT; YFAQEGCSFLYEKLPPSPSSPGNGDCTLLALAQLRGPLSVDVPLVPEGLLTPEASPVKQSFFHYTEKEQN; EIDRLIQQISQLAQGVDRPFSAEAGTGGLEPLGGLEPLNPNLSLSGAGPPVLSLDLKPWKCQELDFLVDP; DNLFLEETPVEDIFMDLSTPDPNGEWGSGDPEAEVPGGTLSPCNNLSPEDHSFLEDLATYETAFETGVST; FPYEGFADELHQLQSQVQDSFHEDGSGGEPTF; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153553
• ORF Size: 2406 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_153553.1, NM_153553.2, NM_153553.3, NM_153553.4, NM_153553.5, NP_705781.1
• RefSeq Size: 3292 bp
• RefSeq ORF: 2409 bp
• Locus ID: 225872
• UniProt ID: Q8BGD7
• Cytogenetics: 19 A
• MW: 87.3 kDa
• Summary: Transcription factor expressed in neurons of the brain that regulates the excitatory-inhibitory balance within neural circuits and is required for contextual memory in the hyppocampus (PubMed:18815592, PubMed:22194569, PubMed:23029555, PubMed:24201284, PubMed:24855953). Plays a key role in the structural and functional plasticity of neurons (PubMed:23172225). Acts as an early-response transcription factor in both excitatory and inhibitory neurons, where it induces distinct but overlapping sets of late-response genes in these two types of neurons, allowing the synapses that form on inhibitory and excitatory neurons to be modified by neuronal activity in a manner specific to their function within a circuit, thereby facilitating appropriate circuit responses to sensory experience (PubMed:24201284, PubMed:24855953). In excitatory neurons, activates transcription of BDNF, which in turn controls the number of GABA-releasing synapses that form on excitatory neurons, thereby promoting an increased number of inhibitory synapses on excitatory neurons (PubMed:18815592, PubMed:22194569, PubMed:24201284). In inhibitory neurons, regulates a distinct set of target genes that serve to increase excitatory input onto somatostatin neurons, probably resulting in enhanced feedback inhibition within cortical circuits (PubMed:24855953). The excitatory and inhibitory balance in neurons affects a number of processes, such as short-term and long-term memory, acquisition of experience, fear memory, response to stress and social behavior (PubMed:18815592, PubMed:22194569, PubMed:23029555, PubMed:24201284, PubMed:27238022). Acts as a regulator of dendritic spine development in olfactory bulb granule cells in a sensory-experience-dependent manner by regulating expression of MDM2 (PubMed:25088421). Efficient DNA binding requires dimerization with another bHLH protein, such as ARNT, ARNT2 or BMAL1 (PubMed:14701734, PubMed:15363889, PubMed:19284974). Can activate the CME (CNS midline enhancer) element (PubMed:14701734, PubMed:15363889).[UniProtKB/Swiss-Prot Function]