Klrd1 (NM_010654) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR220142
Klrd1 (Myc-DDK-tagged) - Mouse killer cell lectin-like receptor, subfamily D, member 1 (Klrd1)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Klrd1
Synonyms CD94
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220142 representing NM_010654
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGTTTCTAGGATCACTCGGTGGAGACTGATGTCTGTCATCTTTGGAATAAAATGTCTTTTCTTGA
TGGTTACTTTGGGAGTTTTGCTGATAAATTCATTTACTATACAGAACATTCAATCAACACCTTCTCCAAC
CACCACTGTAGAATTCCAGGAAGTTTCTGAATGCTGTGTTTGCCTGGACAAGTGGGTTGGGCATCAGTGC
AATTGTTACTTTATTTCCAAAGAAGAAAAGTCTTGGAAAAGAAGCAGAGATTTCTGTGCTTCGCAGAATT
CCAGCCTTCTTCAGCCCCAATCCAGAAATGAATTGAGTTTTATGAACTTCAGTCAAACCTTTTTCTGGAT
TGGAATGCATTATAGTGAAAAAAGAAATGCCTGGCTATGGGAGGATGGCACAGTTCCCTCAAAAGATTTA
TTTCCAGAATTCTCAGTTATCAGGCCAGAGCACTGCATTGTTTACAGTCCAAGCAAAAGCGTTTCTGCTG
AATCCTGTGAAAATAAAAACCGTTACATCTGTAAGAAACTGCCTATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR220142 representing NM_010654
Red=Cloning site Green=Tags(s)

MAVSRITRWRLMSVIFGIKCLFLMVTLGVLLINSFTIQNIQSTPSPTTTVEFQEVSECCVCLDKWVGHQC
NCYFISKEEKSWKRSRDFCASQNSSLLQPQSRNELSFMNFSQTFFWIGMHYSEKRNAWLWEDGTVPSKDL
FPEFSVIRPEHCIVYSPSKSVSAESCENKNRYICKKLPI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010654
ORF Size 537 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010654.4
RefSeq Size 724 bp
RefSeq ORF 540 bp
Locus ID 16643
UniProt ID O54707
Cytogenetics 6 63.42 cM
MW 21.3 kDa
Summary Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010654" in other vectors (4)
SKU Description Size Price
MC208847 Klrd1 (untagged) - Mouse killer cell lectin-like receptor, subfamily D, member 1 (Klrd1), (10ug) 10 ug
$330.00
MG220142 Klrd1 (tGFP-tagged) - Mouse killer cell lectin-like receptor subfamily D member 1 (Klrd1), (10ug) 10 ug
$500.00
MR220142L3 Lenti ORF clone of Klrd1 (Myc-DDK-tagged) - Mouse killer cell lectin-like receptor, subfamily D, member 1 (Klrd1) 10 ug
$600.00
MR220142L4 Lenti ORF clone of Klrd1 (mGFP-tagged) - Mouse killer cell lectin-like receptor, subfamily D, member 1 (Klrd1) 10 ug
$600.00

Plasmid Map for MR220142

Download sequence for

Cloning scheme for MR220142

Circular map for MR220142

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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